Editing Protein Phosphatase 1, Regulatory Subunit 32(PPP1R32)

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Summary	Subcellular Localization of a Novel Alternative Splicing of IIIG9 and Colocalization with PPP1gamma Isoforms. In different cell types can find different regulatory subunits that may constitute targets for therapeutics of diseases such as male infertility, cancer and Alzheimer's disease. A novel alternative splicing isoform of IIIG9 in testis, a known regulator of PPP1, IIIG9sT, and the aim of this study was its further characterization. A specific antibody for IIIG9sT in order to characterize its localization in bovine sperm cells. IIIG9sT-GFP construct in mouse spermatogonia cells (GC-1 cells) and used specific antibodies for each PPP1 isoform for the colocalization studies. Under a fluorescent microscope and a LSM and quantified a high co-localization with PPP1gamma1 and 2 isoforms.
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