Staining Solution for Cells and Tissue

Instruction manual

First Edition (Revised on April, 2016)

Information

The staining technology is one of the important methods to study the pathology. Pathology teaching, scientific research, clinical biopsy and diagnosis can not do without pathological staining technology. In order to display and determine the tissues or cells in the normal structure or in the pathological process of abnormal substances, diseases and pathogens. Appropriate staining methods are for different tissues and different purposes.

Products and application

Product Name	Application	Section type	Format
Hematoxylin eosin (HE) staining solution	Hematoxylin stains the nucleus chromatin and cytoplasm ribosome with purple blue, eosin stains the cytoplasm and the extracellular matrix components with red.	Paraffin section Frozen section Cells	Hematoxylin solution: 100ML Eosin Solution: 100ML
Masson staining solution	Masson staining was mainly used for the differential staining of collagen fibers and muscle fibers. Collagen and cartilage was blue, muscle fiber, cellulose and red blood cell stained red, nuclei stained blue black.	Paraffin section Frozen section	Masson A: 30ML Masson B: 20ML Masson C: 20ML Masson D: 20ML Masson E: 20ML Masson F: 20ML
Alcian blue and periodic acid	AB-PAS staining is used to classify the gastric mucosal	Paraffin section	Alcian blue solution: 20ML
Schiff (AB-PAS) staining solution	epithelial metaplasia of gastric mucosa, and to observe the changes of the morphology of glycogen and mucous substance. Acid mucus was blue, neutral mucus was red, mixed mucus was purple, the nucleus was pale blue	Frozen section	Schiff reagent: 20ML Periodic acid: 20ML Mayer hematoxylin: 20ML Sulfinic acid wash buffer: 100ML
Alcian blue staining solution	Alcian blue belongs to cationic dyes, is the most specific dye for acid mucus.	Paraffin section Frozen section	Alcian blue（PH=2.5) solution: 100ML Nuclear Fast Red solution: 100ML
Triphenyl tetrazolium chloride (TTC) staining solution	Triphenyl tetrazolium chloride (TTC) is a fat soluble light sensitive compound, and normal tissue in the dehydrogenase reaction were red, and tissue ischemia is pale.	Fresh tissue（such as brain tissue）	TTC solution: 100ML
Periodate Schiff (PAS) staining solution	PAS staining can show the polysaccharide, neutral mucus and some acidic mucus substances, as well as cartilage, pituitary, fungi, basement membrane and other substances.	Paraffin section Frozen section Cells	Schiff reagent: 20ML Periodate: 20ML Mayer hematoxylin: 20ML Sulfinic acid wash solution: 100ML
Van Gieson (VG) staining solution	Van Gieson (VG) staining is used to distinguish between collagen fibers and muscle fibers. Staining solution is picric acid and acid fuchsin mixture, the collagen fibers were acid fuchsin stained pink or red, muscle fibers were dyed yellow picric acid	Paraffin section Frozen section	Van Gieson A: 10ML Van Gieson B: 90ML
Oil red O staining solution	Oil red O belongs to azo dyes, it is a strong fat solvent and dye, The fat droplets in the tissue are orange.	Frozen section Cells	Oil red O solution: 100ML
Annona Red-Fast Green staining solution	Annona Red-Fast Green staining  is the common staining of articular cartilage, cartilage and bone tissue.	Paraffin section Frozen section	Annona Red: 100ML Fast Green: 100ML 1% phosphomolybdic acid: 100ML
Nucleic acid staining solution (DAPI)	DAPI stains the double-stranded DNA (dsDNA) with blue.	Paraffin section Frozen section Cells	DAPI: 10ML

Storage and Validity

Different components are stored in the 4°C, -20°C respectively, valid for one year.

Important Note

1.Reagents should be stored according to the instructions.
2.Paraffin section/slice need complete dewaxing.
3.The time of periodate oxidation of tissue sections is within 10 minutes, the ambient temperature is not more than 20°C.