Total Protein/DNA/RNA Extract Customized Service

Instruction manual

First Edition (Revised on April, 2016)

Information

DNA, RNA and protein are three kinds of important biological macromolecules, which are the molecular basis of life phenomena. We provide the custom services to extract total protein, the total RNA and cDNA from animal tissues, primary cells and tumor cells.

Service Contents

	Sample type	Extraction method	Application	UOM	QC results	Period
Total Protein extracts	Tissues and cells	Use self-produced Extraction Kit to extract total protein	For most of the protein analysis experiments and conventional downstream applications and proteomics research	200ug	protein concentration determination by BCA method	3-5 days
Total RNA	Tissues, cells and fresh blood	Use Trizol reagent  to extract and purify the Total RNA	For gene cloning, expression profiling and molecular biology studies	50ug	Results of the RNA sample concentration, total content, OD value	3-5 days
cDNA	Tissues, cells and fresh blood	Extraction of total RNA, and RNA reverse transcription to cDNA	For gene cloning, expression profiling and molecular biology studies	50ug	Results of the cDNA concentration,  OD value	5-7 days

Sample requirements

Customers provide fresh tissue, blood and cells,and sent to our lab ASAP by dry ice.
Note: Need to provide the number and the status of cells  (for the assess the amount of lysate buffer).

Service Results

Provide the desired extract to the customer;
Test data;