DAB Chromogen Kit

Instruction manual

First Edition (Revised on April, 2016)

INTENDED USE

DAB Chromogen Kit is a widely used chromogen for immunohistochemical staining and immunoblotting.

PRINCIPLE

DAB (3,3N-Diaminobenzidine Tertrahydrochloride) is one of the most important chromogens for peroxidase. In the presence of peroxidase enzyme, a dark brown precipitate will result.

REAGENTS

Reagents	Amounts	Ingredients
Chromogen A (1×)	5mL*2	Peroxide
Chromogen B (50×)	200μL	DAB

STORAGE OF THE KIT

It is recommended to store chromogen A and chromogen B at 4oC. The expiration date of the kit is about 6 months.

ASSAY PROCEDURES

1. Preparation: Mix 1mL chromogen A with 20μL chromogen B thoroughly, and then add this solution to the sample. Observe under a microscope to verify the ending time. Generally, 10 sec to 15 min is enough.
2. Fully soak sample with distilled water to end the reaction.
3. Chromogen B becoming darker has no effect when use.
4. DAB is a suspected carcinogen. Please take appropriate care when using it.