Product: | PGA Monoclonal Antibody |
Target molecule: | Pepsinogen A (PGA) |
Host: | Mouse |
Source: | Ascites |
Purification: | Affinity Chromatography |
Isotype: | IgG2b, Kappa |
Traits: | Liquid |
Concentration: | 500µg/mL |
Buffer: | 0.01M PBS, pH7.4, containing 0.05% proclin-300, 50% glycerol. |
Pairing: | RSA165Hu21 (capture antibody) is recommended to pair with RSA165Hu22 (detection antibody) in immunoassay. |
Applications: | Recommended use: Recommended to be used in immunoassays for recognition of human PGA. Tested application: Capture antibody (1-4µg/mL) in ELISA assay. Detection antibody (0.5-10µg/mL) in WB. Detection antibody (5-40µg/mL) in IHC. |
Storage: | Aliquot and store at -20°C for 12 months. Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. |
Identification of Specificity:
Figure 1. ELISA
Purified antigen and natural samples were tested in ELISA with pairing antibodies of RSA165Hu21 and RSA165Hu22.
Figure 2. WB
Sample: | A. Recombinant Human PGA | B. Porcine Stomach |
Figure 3. IHC
Sample: | A. Human Brain Tissue | B. Human Glioma Tissue |
C. Human Thyroid Cancer Tissue | D. Human Kidney Tissue | |
E. Human Prostate Gland Tissue | F. Human Prostate Gland Cancer Tissue | |
G. Human Esophagus Cancer Tissue | H. Human Stomach Tissue | |
I. Human Pancreas Cancer Tissue | J. Human Uterine fibroids Tissue |