Fluorescent Antibodies from Cloud-Clone Corp.

The remarkable 2014 Novel Prize was announce, scientists from America and German, Eric Bezig, Stefan W. Hell and W. E. Moerner had won the chemistry prize, for their enormous contributions in microscopy, they had used fluorescent molecules in microscopy to break the physical limit (0.2 micrometer) of optical microscopy filed which was defined in 1873, and the limit of this field had been brought to the level of nanometer by this work.

One main research of this prize is single-molecule fluorescent microspogy, which is researched by Eric Betzig and W. E. Moerner. This technique had been used in several research fields, including biological field. For instance, it has been applied in researches of the formation processes of neural synapses, aggregation and degradation of specific proteins in different diseases, metabolic processes of medicine in cells, etc. In these researches, there are lots of common used fluorescent markers, for instance, fluorescein isothiocyanate (FITC), cyanine dyes (Cy2, Cy3, Cy5) and so on.

Cloud-Clone Corp. had developed various of labeled antibodies based on normal used monoclonal and polyclonal antibody, for instance, biotin-labeled antibody, HRP-labeled antibody, FITC-labeled antibody, and so on. The FITC-labeled antibody could be used in Immunonological Histological Chemistry (IHC)，Immunofluorescence (IF), Fluorescence Molecular Tomography in living small animals, etc.

The application of FITC-labeled antibody in IHC is shown below by using FITC-linked monoclonal antibody to human anterior gradient protein 2 (MAC285Hu81), the tissue used in the experiment is human gastric cancer tissue (Figure 1).

The application of FITC-labeled antibody in immunofluorescence is shown below. by using FITC-linked monoclonal antibody to human RAB5A, member RAS oncogene family (MAK305Hu81) and 4',6-diamidino-2-phenylindole (DAPI), the experiments were performed in mouse bone marrow-derived macrophages (BMDM) (Figure 2 and Figure 3).

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