Heat Shock Protein 90a Multi-Species ELISA Test Kit
Heat shock proteins (heat shock protein, HSP) are a group of proteins rapidly induced by heat shock. An important feature in the biological world is that they are highly conserved in evolution. In recent years, HSP90a is the first one to be discovered and defined as the tumor marker by Chinese among more than 30 tumor biomarkers for tumor diagnosis worldwide. The others were defined by scientists in other countries. Cloud-Clone Corp. has concerned about this biological hotspot. It is well known that the HSPs are highly conserved. By aligning the sequences of the full length HSP90a from different species, such as human, rat, mouse, Guinea pig, pig, sheep, house, etc, the R&D Center of USCN found that the identities among those proteins are all above 99%. Based on the characteristics of these proteins and the feature of antibody preparation, Cloud-Clone Corp. developed a new type of ELISA detection kit- Multi-Species Kit, which recognizes highly homologous proteins. Researchers can only purchase just one kit to detect the target protein for samples from multiple species.
Because protein is homologous, the kit can detect the same protein in different samples from different species. But as there exists some differences in the protein’s structure or sample properties of different species, so further experiments are also needed to identify the proper detection range to be suitable for quantitative measurement the samples from muli-species. As to the multi-species kit for HSP90a, the catalog number is SED523Mi，Enzyme-linked Immunosorbent Assay Kit For Heat Shock Protein 90kDa Alpha A1 (HSP90aA1), the “Mi” means the kit can detect samples from multiple species. Also, the manual will specify the species which can be detected by the kit, which including: Hu/Mu/Ra/Gu/Po/Ov/Eq.
In order to meet the research demands, Cloud-Clone Corp. began to develop the HSP90a polyclonal antibody(PAD523Mi01). It is widely applicable for the samples from multiple species origin. In addition, Cloud-Clone Corp. labeled antibodies with biotin and FITC to obtain antibodies (PAD523Mi71 and PAD523Mi81). These conjugated antibody not only can recognize HSP90a specifically, but also can be more convenient for an in vivo detection of HSP90a. Take PAD523Mi01 as an example, the IHC test and WB test verified the specificity of PAD523Mi01. The images are shown in Fig. 1 and Fig. 2, respectively. Therefore, the quality is guaranteed. Please rest assured purchase.
In addition, according to the report in 2009 "American Academy of Sciences" (PNAS), the HSP90a blood levels is positively correlated with the degree of malignancy, the HSP90a level in tumor cells is 2-7 fold higher than that in healthy ones, which showed that this protein has a great potential to become a tumor biomarker. Cloud-Clone Corp. further tests the PAD523Mi01 by IHC-P with tissues from human breast cancer and thyroid cancer, respectively. The results are shown in Fig. 3.
According to the molecular weights, heat shock proteins can be divided into five categories, including HSP100, HSP90, HSP70, HSP60 and small molecules. Cloud-Clone Corp. has developed a series of respective recombinant proteins, antibodies and quantitative detection kits and other related products. The stories of human breast cancer human thyroid cancer HSPs are to be updated in the next topics. Your attenton is invited
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