Instant ELISA Kit Developed by Cloud-Clone Corp.

Cloud-Clone Corp.

Generally, It needs about 4-5 hours for researchers to accomplish the experiment while using the common used sandwich ELISA kit. So, in order to improve the efficiency of research, Cloud-Clone Corp. had done lots of work in this area. Recently, the instant ELISA kit is pushed into the market. For instance, Instant ELISA Kit for Isocitrate Dehydrogenase 1, Soluble, (IDH1), the catalog number is IEH839Hu.

Comparing to the normal ELISA kit for IDH1 (SEH839Hu), there are lots of advantages for the instant ELISA kit. For instance, the experimental time is short, sample volume needed for detection is small, reagents in the kit are ready to use, the sensitivity is high, et al. The comparison of normal kit and instant kit is shown in Table 1.

Table 1. Comparison of instant kit and normal kit

Characteristics Instant ELISA KIT Normal ELISA KIT
Detection reagent A,B Working solution, ready to use Concentrated solution, prepare to working solution before use
Experimental time 3h 4.5h
Volume of sample 50ul 100ul
Experimental step 7 8

ELISA kit for Isocitrate Dehydrogenase 1 (IDH1) (SEH839Hu) is developed based on the importance of IDH1 in tumor research in recent years. The researches show that IDH1 is an oxidordeuctase. As the receptor of NADP+, it mainly exists in cytoplasma and peroxysome. It is reported that IDH1 is closely related to several kinds of cancer, for instance, acute leukemia, colon cancer and glioma, and so on.

Regarding to the research of IDH1, Peking Union Medical College and Chinese Academy of Medical Sciences (CAMS) discovered that IDH1 could be used as biomarker for cancer diagnosis and prognosis, and the result is published in Molecular & Cellular Proteomics. Based on this, the same research team tested a large quantity of clinical samples to make further systematic investigations of the function of IDH1 in cancer diagnosis, and this result is published in Clininal Cancer Research. The research shows that the concentration of IDH1 in cancer patient are significant higher than it is in healthy people. In the meanwhile, after comparing the sensitivity and specificity of IDH1 and traditional tumor markers (CA125, Cyfra21-1 and CEA) in cancer detection, they found that IDH1 is a better biomarker in the diagnosis of certain cancer. In sum, IDH1 could be used as an efficient and potential biomarker in the screening and diagnosis of certain cancer

The IDH1 detection kit (SEH839Hu) used in these researches is from Cloud-Clone Corp., which is a highly professional company, and always focuses on the demand of research. Moreover, Cloud-Clone Corp. has developed instant ELISA kit for IDH1 (IEH839Hu) with develped technology and high-sensitivity antibodies, and it has already made its way into scholars’ view, now it has been a candidate for cancer screening kit.

For more information, please visit http://www.cloud-clone.us/.