mini kit- less sample, higher sensitivity
Elisa is a rapid, sensitive and accurate quantitative analysis method. It will need 100ul of sample for detection, while sometimes it’s just hard to get enough samples, especially when the concentration of target protein in the samples is low (such as: all kinds of cytokines). Then the normal ELISA kit will not be suitable. Now Cloud-Clone Corp. has developed Mini ELISA kits which require less samples (25ul~50ul), with the same procedure and experimental time, and its result is the same or even better than conventional kits. Now let’s take SEA079Mu (ELISA Kit for Interleukin 6 (IL6)) and MEA079Mu (Mini ELISA Kit for Interleukin 6 (IL6)) as an example to compare the whole process. The procedure is as follows.
Table 1. Plate layout of two kits
| 1 | 2 | |
| A | Blank | tissue 1 |
| B | STD1(1.56pg/ml) | tissue 2 |
| C | STD2(3.12pg/ml) | tissue 3 |
| D | STD3(6.25pg/ml) | tissue 4 |
| E | STD4(12.5pg/ml) | cell lysate 1 |
| F | STD5(25pg/ml) | cell lysate 2 |
| G | STD6(50pg/ml) | cell lysate 3 |
| H | STD7(100pg/ml) | cell lysate 4 |
At the same time, in order to compare those two kits, the researcher in our lab has designed an experiment. In the experiment, the experimental condition, samples, operator and operating mode of those two kits are totally same, the operation is synchronized. Plate layout of two kits are shown in table1, the raw data of the two kits is shown as follows.
Table 2. The result of MEA079Mu
| 1 | 2 | |
| A | 0.049 | 0.611 |
| B | 0.086 | 0.443 |
| C | 0.174 | 0.945 |
| D | 0.315 | 0.378 |
| E | 0.532 | 0.518 |
| F | 0.971 | 0.689 |
| G | 1.679 | 2.238 |
| H | 2.753 | 0.201 |
Table 3. The result of SEA079Mu
| 1 | 2 | |
| A | 0.053 | 0.662 |
| B | 0.094 | 0.504 |
| C | 0.215 | 1.004 |
| D | 0.322 | 0.412 |
| E | 0.593 | 0.563 |
| F | 1.094 | 0.745 |
| G | 1.686 | 2.287 |
| H | 2.774 | 0.237 |
We had analyzed the raw data, the r^2 of MEA079Mu is 0.9999, the r^2 of SEA079Mu is 0.9991, their OD data of standard curve is close. After the calculation, the difference between the concentration of the same sample is less than 10%.
Above all, mini kits could detect samples as same as conventional kits while cost less samples and reagents. Then for customers, if their samples are very precious, and the volume are limited, then the mini kit would be one of their best choices.
For more information, please enter http://www.cloud-clone.com/