New Gene Product ---- Taq DNA polymerase

After recombinant protein Primer Pairs, Cloud-Clone Corp. released another molecular cloning tool --Taq DNA polymerase.

Taq DNA polymerase was originally from Thermus Aquaticus (Taq) yT1 strains isolated from volcanic hot springs bacterium in Yellowstone National Forest Park in 1969. This enzyme gene contains 2496 bases, encoding 832 amino acids. It is a heat-stable DNA polymerases with molecular weight of approximately 94kDa. Its optimal operating temperature is 75-80°C. At conventional PCR extension phase (72°C), about 1000 nucleotides can be extended every 10 seconds. With thermal stability, Taq DNA polymerase will not be inactivated at temperature above 90°C, therefore that makes PCR get rid of complex processes: someone taking care of it and adding enzyme in each cycle .

The Taq DNA polymerase released by Cloud-Clone can be used in conventional gene amplification, cloning identification. It is mainly provided in the form of PCR Mix system. The system comprises Taq DNA polymerase, dNTPs, Mg2 +, reaction buffer, etc. PCR amplification reaction starts as long as system-specific primers and template are added, so that experimental procedure would be minimized, thereby operation errors and contamination would be reduced. The reagent has many advantages, say, simple, rapid, high sensitivity, good stability, etc. It is not only applicable to conventional PCR amplification, but also has more obvious advantages in genetic testing in large quantities.

In procedure of molecular cloning process, combined with PCR Mix, Primer Pairs and cDNA libraries of various species in different organizations, it is convenient to obtain gene sequence of different target protein.

For more information, please visit: www.cloud-clone.com.