Analysis of the BCA Protein Assay kit of Cloud-Clone Corp.

Currently,theworld’s most commonly used kit to detect the protein concentration is BCA protein assay kit and Bradford protein assay kit.And BCA method alwayswas knows as simple,stable and sensitive.

  Thecompatibility of protein concentration were determined by BCA method is alsovery good.It is not affected by most of other components in the sample.For lessthan 5% of SDS,Triton X-100,Tween 20,Tween 80, it has good compatibility.Thereis a good linear relationship in the 50~1000μg/ml concentrationrange,and the minimum detection limit was 25μg/ml.

  Certainly,Cloud-Clone Corp. alsodeveloped its own BCA Protein Assay Kit.Now, The Cloud-Clone Crop. kits werecompared with Thermo kit by standard curve, standard OD value(450nm),Sample ODvalue(450nm),internal control recoveries and coefficient of variation.

1. Comparison of the original OD(450nm)

  Thecomparison table of Cloud-Clone Crop. kits and Thermo kits original OD is asfollow:

2. Comparison of the tandard curve and standardOD value

  After thecomparison of Cloud-Clone Crop. kits and Thermo kits,and the analysis ofexperimental results,we can known that thestandard curve of Cloud-Clone Crop.kits and Thermo kits is good.The R2 is 0.9995.

3. Comparison of the sample OD value and internal control recoveries

  Using theCloud-Clone Crop. BSA standards as internal control of Thermo kit,the highvalue (1000ug / ml) recovery was 95.5%,the low value(125ug / ml) recovery was110.7%; And using the Thermo kit BSA standards as internal control of Cloud-CloneCrop. kit,the high value (1000ug / ml) recovery was 97.57%,thelow value(125ug / ml) recovery was 105.98%.(Internalcontrol recoveries generally should be controlled at 80% to 120%)

4. Comparison of the coefficient of variation

  Using Cloud-CloneCrop. kit and Thermo kit to determining B970,ZBP1-1, A503, SLIT3-3 and PE4,we can obtain the coefficient of variation lessthan 10%.

Through the comparison of standard curve,standard OD value(450nm),Sample OD value(450nm),internalcontrol recoveries and coefficient of variation from Thermo kits to Cloud-CloneCrop kits,we can find out that,the BCA kit which from Cloud-Clone Crop. has agood linear relationship,The R2 can achieve 0.9995,the internal controlrecoveries can be controled between 95%-111%,and the coefficient of variationof less than 10%.These data demonstrated BCA protein assay kit from Cloud-CloneCrop, had a high stability and high sensitivity.

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