The Detection and Application of Complement C3 in Serum and Plamsa

Complements are mainly distributed inbody fluids and cell surface of human or animal. After activation, they couldmediate immune and inflammatory responses, they are also known as thecomplement system.

Complement system consists of nearly 40kinds of ingredients, most of them are glycoproteins.There are 13 inherentcomponents, over 10 regulatory proteins, and more than 10 kinds of complementreceptors.

Complementcan’t be saved in hot temperature,when it reaches 56 degrees, most of the complementswill lose the activity after 30 minutes. Complement is difficult to preserve,in freeze and dry environment, they can bestored in relatively long time.

ComplementComponent 3(C3) is a core component.The molecular weight is 195000, and it’scoded by C3 gene on chromosome 19.C3 is the largest complement component ofblood level, it’s mainly synthesized in macrophages and liver.It has anindispensable role in complement classical and bypass activation pathways. Itcan be cleaved into two fragments like C3a and C3b.

For C3, the Cloud-Clone, Corp.developed recombinant C3 RPA861Hu01, it can be used in SDS-PAGE; WB; ELISA; IP etc.Polyclonal antibodies PAA861Hu01, Biotin-labeled polyclonal antibody PAA861Hu71and Elisa Kit SEA861Hu.

The imunogene of SEA861Hu is Ser23~Glu266, the detectionrange of the kit is 7.813-500ng/ml, the sensitivity is 2.8ng/ml.During thedevelopment, different kind of samples were detected. According to our results,the blood samples need to be diluted 20,000 times,the concentration of C3 is0.618~6.067mg/ml,a few are much lower, while the samples’ background is unknown.

Serumsamples is preferred when detecting C3 in the blood. Because after anticoagulant,some fibrin in serum samples may interfere the detection.

Thereare significant clinical significance in detecting C3 in blood. The early stageof some acute inflammatory or infectious diseases, such as myocarditis, myocardialinfarction and arthritis. And in other diseases like:cirrhosis,early or lateacute glomerulonephritis,the content of C3 will have significantfluctuations.So,the dynamic changes of Complementwill be more and more important in clinical monitoring.

Simultaneously, there are a clinical significance ofdetecting C3 in urine,too.C3 doesn’t exist in urine conventionally.But when inglomerular disease,due to the capillary wallthickening,deforming,fracturing,structural damage and permeability raising,C3will appear in urine.Therefore, when we detected kidney related ailments,content of C3 in urine can be used as diagnostic reference.

Insummary,detecting the content of C3 in blood and urine has important roles inmany diseases,such as hepatitis,Systemic Lupus Erythematosus and kidneydisease.

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