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How to Detect HMGB1 (High Mobility Group Box 1) in-vitro

Cloud-Clone Corp.

High Mobility GroupProtein 1, also known as amphotericin. It’s encoded by HMGB1 genes. It’s namedwith HMGB1 due to the high mobility in SDS-PAGE.

Now we treat humanHMGB1 as an example to have a brief introduction on High Mobility Group Protein1.

Protein:

Like Histones, HMGB1is the most important chromatin protein, it plays significant roles inmaintaining and stabilizing nucleosome DNA recombination, replication,transcription, repair etc. In addition, it also functions in infection,inflammation and immune reactions. HMGB1 is also one of the focus ofinflammation injury. Some scholars proposed HMGB1 as a DNA vaccine adjuvant andcancer therapeutic target.

According UniprotMaster HMGB1 link[A1] , http://www.uniprot.org/uniprot/P09429, weprepared three HMGB1 fragment. It’s Met1 ~ Glu215(RPA399Hu01), Pro9 ~ Arg163 (RPA399Hu02),Met1 ~ Glu215(RPA399Hu61).RPA399Hu01 and RPA399Hu01 is expressed in E.coli, and RPA399Hu61 is expressedby 293F. Thus the researchers could choose different products according totheir experimental purposes.


Antibody: Immunizingmice and rabbits with proteins above to get monoclonal and polyclonalantibodies. Then purified with affinity chromatography to get high purityantibodies. The titer of monoclonal antibody MAA399Hu22 could reach 81w.

Kit:

After obtainingmonoclonal and polyclonal antibodies, we developed Elisa kits for HMG1. Kit iscoated with mouse monoclonal antibodies, and detected with rabbit polyclonalantibodies. [A1] The content of HMG1 varies in normal and diseasemodels, the Cloud-Clone. Corp. detected many samples, from 46 apparently healthy people, the concentration isfrom 72 ~ 582ng / ml, for the customer’s reference.

In addition, only withSEA399Hu, we currently have 24 literatures which cited the product.

Reference TitleReference TitleSource MagazineSource Magazine Links
IGF-1 alleviates ox-LDL-induced inflammation via reducing HMGB1 release in HAECs Biomark Insights http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3623607/
Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with AutismBiomark Insights http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3623607/
ATF3 Protects against LPS-Induced Inflammation in Mice via Inhibiting HMGB1 ExpressionEvidence-based Complementary and Alternative Medicinehttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3770047/
High mobility group B1 levels in sepsis and disseminated intravascular coagulationACTA biochimica polonicahttp://www.actabp.pl/pdf/4_2012/561.pdf
Clinical relevance of single nucleotide polymorphisms of the high mobility group box 1 protein gene in patients with major trauma in Southwest ChinaSurgeryhttp://www.ncbi.nlm.nih.gov/pubmed/22047946
Is HMGB1 a New Indirect Marker for Revealing Fibrosis in Chronic Hepatitis and a New Therapeutic Target in Treatment?Viral Immunologyhttp://online.liebertpub.com/doi/abs/10.1089/vim.2010.0080
Diagnostic value of serum concentrations of high-mobility group-box protein 1 and soluble hemoglobin scavenger receptor in brucellosisMicrobiology and Immunologyhttp://www.ncbi.nlm.nih.gov/pubmed/23278480
Elevated serum levels of high mobility group box protein 1 (HMGB1) in patients with ankylosing spondylitis and its association with disease activity and quality of lifeRheumatology Internationalhttp://www.ncbi.nlm.nih.gov/pubmed/23143556
Correlation between serum high-mobility group box-1 levels and high-sensitivity C-reactive protein and troponin I in patients with coronary artery diseaseExp Ther Medhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3094252/‍‍
High plasma levels of high mobility group box 1 is associated with the risk of sepsis in severe blunt chest trauma patients: a prospective cohort studyJ Cardiothorac Surg.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132233/
Effects of Single or Combined Treatments with Radiation and Chemotherapy on Survival and Danger Signals Expression in Glioblastoma Cell Lines‍‍BioMed Research Internationalhttp://www.hindawi.com/journals/bmri/2014/453497/abs/
The Alarmin Concept Applied to Human Renal Transplantation: Evidence for a Differential Implication of HMGB1 and IL-33‍‍‍‍PLOS ONEhttp://www.ncbi.nlm.nih.gov/pubmed/24586382
High-mobility group box-1, promising serological biomarker for the distinction of human WNV disease severityVirus Researchhttp://www.ncbi.nlm.nih.gov/pubmed/25197038
Increased VEGF and Decreased SDF-1α in Patients with Silent Brain Infarction Are Associated with Better Prognosis after First-Ever Acute Lacunar Stroke Journal of Stroke and Cerebrovascular Diseaseshttp://www.ncbi.nlm.nih.gov/pubmed/25601176
Plasma levels of high mobility group box 1 increase in patients with posttraumatic stress disorder after severe blunt chest trauma: a prospective cohort studyJournal of Surgical Researchhttp://www.ncbi.nlm.nih.gov/pubmed/25016440
Wogonin Inhibits Tumor-derived Regulatory Molecules by Suppressing STAT3 Signaling to Promote Tumor ImmunityJournal of Immunotherapyhttp://www.ncbi.nlm.nih.gov/pubmed/25962106 
Increased VEGF and Decreased SDF-1α in Patients with Silent Brain Infarction Are Associated with Better Prognosis after First-Ever Acute Lacunar Stroke J Stroke Cerebrovasc Dishttp://www.ncbi.nlm.nih.gov/pubmed/25601176‍‍‍‍
Plasma HMGB-1 Levels in Subjects with Obesity and Type 2 Diabetes: A Cross-Sectional Study in ChinaPLOS ONEhttp://www.ncbi.nlm.nih.gov/pubmed/26317615
High mobility group box 1 gene polymorphism is associated with the risk of postoperative atrial fibrillation after coronary artery bypass surgeryjournal of cardiochoracic surgeryhttp://www.ncbi.nlm.nih.gov/pubmed/26109393
Overexpression of HMGB1 A-box reduced lipopolysaccharide-induced intestinal inflammation via HMGB1/TLR4 signaling in vitroWorld J Gastroenterolwww.ncbi.nlm.nih.gov/pubmed/26167076
Low Shear Stress Induced HMGB1 Translocation and Release via PECAM-1/PARP-1 Pathway to Induce Inflammation ResponsePLoS Onewww.ncbi.nlm.nih.gov/pubmed/25793984

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