The in-vitro Detection and Extraction of Various Histones

Histone is a kind of strongly basic proteinexists in chromatin of eukaryotic cell nucleus[A1] , which is the basic structure protein ofeukaryotic chromosome. The DNA is compressed by Histones and together withHistones to form nucleosomes. If there is no histone[A2] , the chromosomal DNA will be unlocked and will be scattered longDNA chains.[A3] 

It has greatsignificances to have researches on Histones. The abnormal level of Histoneswill lead to diseases, such as chronic kidney disease, etc. Also Histone modificationis related to immune regulation, autoimmune diseases, cancer, neurologicaldiseases and endocrine diseases.

There are six types ofHistones: H1, H2A, H2B, H3, H4 and archaeal Histones, which are rich of basicpositive charged amino acids. While the DNA are negatively charged. In this situationthey will interact, the DNA is winding with Histones, forming DNA- histonecomplexes. H2A, H2B, H3 and H4 are called core Histones, and H1 and H5 areknown as linker Histones. Core Histones exist in dimeric form.

H2A, H2B, H3, H4 arenon-specific proteins in human, rat and mice. The Cloud-Clone. Corp. developedmultispecies kits for the four core histones.

SEA282Mi          ELISA Kit for Histone H2A (H2A)

SEA356Mi          ELISA Kit for Histone H2B (H2B)

SEA285Mi          ELISA Kit for Histone H3 (H3)

SEA289Mi          ELISA Kit for Histone H4 (H4)

As Histones are a kindof nuclear proteins, so in experiments such as Elisa, the samples preparationis particularly important. Inappropriate sample processing has significantimpact on experimental results.

For extraction ofHistones, we provide the composition of extraction reagents for researchers’reference.

 50 mM Tris (pH 7.4), 300 mM NaCl, 10% (w/v)glycerol, 3 mM EDTA, 1 mM MgCl2, 20 mM glycerophosphate, 25 mM NaF, 1% TritonX-100, 25 ug/mL Leupeptin, 25 ug/mL Pepstatin, and 3 ug/mL Aprotinin.

For moreinformation, please enter http://www.cloud-clone.us/.