The high efficient technology on gene seamless-splicing

WithPCR technology develops, The door to the study of the essence of life hasopened.The PCR technology has become one of the most important technologies inthe last century.With the PCR ,we can amplify genes in vitro. Owning the targetgene fragment is the beginning for genetic modification,we need an efficienttool for gene processing----Gene-splicing techniques.In experiments,Gene-splicing techniques are play an impoertent role in them,eg: tranforminggene,tranforming plasmid, and tranforming species.

  In the 1989 ,Horton, etal. proposed the SEO technology.Geneswere spliced by over lap extension. In the 1991, Lebedenko et al. devised the SDLtechnology. Genes were spliced by directed ligation. Recently,some commonlygene-splicing technology are based on these two techniques.They are able toachieve the desired goals,but there are some mistakes about them,as follows:

   1.The introduction of exogenous fragment;

   2.Frameshift;

   3.Complicated operation and the low successrate;

   4.A splicing reaction can together. with twogene fragments.

  The CLOUD-CLONE CROP has researched thenuclease and reaction system more than seven years.We will supply a new productwhich is used for splicing the target gene fragment.It has broken thelimitations of traditional techniques,as follows:

   1.A splicing reaction could completed 3-5different fragments.

   2.It suits for kind of fragments whichdistribute in the range of 200-8000bp.

   3.Higher splicing rate, it is more than 85%.

   4.It doesn’t bring exogenous fragment.

  A new nuclease paly an importent role in thistechnique.It could non-specifically distinguish the linear DNA molecule’s 5’-3’terminals,and digest 16 base from 5’ to 3’terminal becoming the cohesiveends.If the target gene and linear target vector own the same cohesiveends,they cloud link together and become a complete plasmid by DNArenaturation.

  The CLOUD-CLONE CROP provides gene splicing,vector construction, library construction and other molecular biologycustomized service.Welcome to consult us!

  More products, please visit: www.cloud-clone.us.