The preparation of phosphorylate polyclonal and monoclonal antibodies

Recently,researches on the protein post-translational modification have becomea hot spotin area of the proteomics.The protein post-translational modification referringsome amino acids in the protein have been modified. There are kinds of proteinmodification,eg, Phosphorylation, glycosylation, methylation,acetylation, etc.Theprotein post-translational modification is one of the most important proteinmodifications.The protein post-translational modification consists of proteinkinases and phosphatases,which take part in a reversible process.It has foundthat the protein post-translational modification join in variety ofphysiological and pathological processes in vivo, such as glucose metabolism,photosynthesis, cell growth, gene expression, neurotransmitter synthesis andrelease, and even cancer, etc.The protein post-translational modification playan important role in the signaling processes in cells.It is the center of cellactivity.The phosphorylated antibodies could specifically recognize the proteinphosphorylation sites.It acts as an important detection tool for detecting theprotein whether has been phosphorylated.This process don’t use radioactivesubstances and could analysis protein phosphorylation.

   Phosphorylatedand non-phosphorylated antibody has been used in the different areas.There aresome differents form them.As follows:

   1.The phosphorylation antibody is used fordetecting the protein whether has been phosphorylated.

   2.The non-phosphorylated antibody is usedfor detecting the target protein tissue specific,cell status,an so on.It isalso used for internal control in the phosphorylation testing.

   3.Synthesis of non-phosphorylatedantibodies:

Proteinor Conjugated polypeptide->Immunized animals->Harvest Antibody(Cellfusion->Preparation of ascites->Monoclonal Antibody Purification)

   4.Synthesis of  phosphorylation antibody:

Syntheticpeptides containing phosphorus acidification site->Artificial in vitrophosphorylation->Polypeptide conjugated carrier->Immunizedanimals->Harvest Antibody(Cell fusion->Preparation ofascites->Monoclonal Antibody Purification).                                                                                  The CLOUD-CLONE CROP has committed to research the modifiedantibodies.We supply phosphorylation, acetylation, ubiquitination, and othermodified antibodies custome services.We guarantee the purified antibody ELISAtiter ≥1: 64,000 and cross-reacting withnon-modified polypeptide <10%.

   More products, please visit: www.cloud-clone.us.