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Neutrophil Elastase (NE)

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ELANE; HLE; HNE; ELA2; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; Bone Marrow Serine Protease; Leukocyte Elastase; PMN Elastase

Neutrophil Elastase (NE)
CCC A181
HGNC 3309
MGI 2679229
PubMed 20179351
RGD 1307968
UniProt P08246
Wiki ELA2

Neutrophil elastase is a serine protease in the same family as chymotrypsin and has broad substrate specificity. Secreted by neutrophils during inflammation, it destroys bacteria and host tissue. As with other serine proteases it contains a charge relay system composed of the catalytic triad of histidine, aspartate, and serine residues that are dispersed throughout the primary sequence of the polypeptide but that are brought together in the three dimension conformation of the folded protein. The gene encoding neutrophil elastase, ELA2, consists of five exons.  The neutrophil form of elastase is 218 amino acids long, with two asparagine-linked carbohydrate chains. It is present in azurophil granules in the neutrophil cytoplasm. There appear to be two forms of neutrophil elastase, termed IIa and IIb.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA181Hu01 Recombinant Neutrophil Elastase (NE) Positive Control; Immunogen; SDS-PAGE; WB.
APA181Hu01 Active Neutrophil Elastase (NE) Cell culture; Activity Assays.
RPA181Hu02 Recombinant Neutrophil Elastase (NE) Positive Control; Immunogen; SDS-PAGE; WB.
RPA181Hu03 Recombinant Neutrophil Elastase (NE) Positive Control; Immunogen; SDS-PAGE; WB.
RPA181Hu04 Recombinant Neutrophil Elastase (NE) Positive Control; Immunogen; SDS-PAGE; WB.
APA181Hu03 Active Neutrophil Elastase (NE) Cell culture; Activity Assays.
Antibodies PAA181Hu01 Polyclonal Antibody to Neutrophil Elastase (NE) WB; IHC; ICC; IP.
MAA181Hu22 Monoclonal Antibody to Neutrophil Elastase (NE) WB; IHC; ICC; IP.
Assay Kits SEA181Hu ELISA Kit for Neutrophil Elastase (NE) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA181Mu01 Recombinant Neutrophil Elastase (NE) Positive Control; Immunogen; SDS-PAGE; WB.
RPA181Mu02 Recombinant Neutrophil Elastase (NE) Positive Control; Immunogen; SDS-PAGE; WB.
EPA181Mu61 Eukaryotic Neutrophil Elastase (NE) Positive Control; Immunogen; SDS-PAGE; WB.
APA181Mu61 Active Neutrophil Elastase (NE) Cell culture; Activity Assays.
Antibodies PAA181Mu01 Polyclonal Antibody to Neutrophil Elastase (NE) WB; IHC; ICC; IP.
PAA181Mu02 Polyclonal Antibody to Neutrophil Elastase (NE) WB; IHC; ICC; IP.
Assay Kits SEA181Mu ELISA Kit for Neutrophil Elastase (NE) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA181Ra01 Recombinant Neutrophil Elastase (NE) Positive Control; Immunogen; SDS-PAGE; WB.
APA181Ra01 Active Neutrophil Elastase (NE) Cell culture; Activity Assays.
Antibodies PAA181Ra01 Polyclonal Antibody to Neutrophil Elastase (NE) WB; IHC; ICC; IP.
MAA181Ra21 Monoclonal Antibody to Neutrophil Elastase (NE) WB; IHC; ICC; IP.
Assay Kits SEA181Ra ELISA Kit for Neutrophil Elastase (NE) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Canis familiaris; Canine (Dog)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA181Ca01 Recombinant Neutrophil Elastase (NE) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA181Ca01 Polyclonal Antibody to Neutrophil Elastase (NE) WB; IHC
MAA181Ca21 Monoclonal Antibody to Neutrophil Elastase (NE) WB; IHC; ICC; IP.
Assay Kits SEA181Ca ELISA Kit for Neutrophil Elastase (NE) Enzyme-linked immunosorbent assay for Antigen Detection.
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  8. , (In) Protein degradation in health and disease, Ciba Foundation Symposium, pp.75:51-68, Excerpta Medica, Amsterdam and Oxford (1980)
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  10. "Use of proteinase 3 purified by reverse phase HPLC to detect autoantibodies in systemic vasculitis."J. Immunol. Methods 180:25-33(1995) [PubMed] [Europe PMC] [Abstract]
  11. "Myelomonocytic cell lineage expression of the neutrophil elastase gene."J. Biol. Chem. 263:2543-2547(1988) [PubMed] [Europe PMC] [Abstract]
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  14. "Human leukocyte elastase and cathepsin G are specific inhibitors of C5a-dependent neutrophil enzyme release and chemotaxis."Exp. Dermatol. 13:316-325(2004) [PubMed] [Europe PMC] [Abstract]
  15. "A novel notch protein, N2N, targeted by neutrophil elastase and implicated in hereditary neutropenia."Mol. Cell. Biol. 24:58-70(2004) [PubMed] [Europe PMC] [Abstract]
  16. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
  17. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
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  19. "The refined 2.3-A crystal structure of human leukocyte elastase in a complex with a valine chloromethyl ketone inhibitor."FEBS Lett. 234:367-373(1988) [PubMed] [Europe PMC] [Abstract]
  20. "X-ray crystal structure of the complex of human leukocyte elastase (PMN elastase) and the third domain of the turkey ovomucoid inhibitor."EMBO J. 5:2453-2458(1986) [PubMed] [Europe PMC] [Abstract]
  21. "Mutations in ELA2, encoding neutrophil elastase, define a 21-day biological clock in cyclic haematopoiesis."Nat. Genet. 23:433-436(1999) [PubMed] [Europe PMC] [Abstract]
  22. "Mutations in the gene encoding neutrophil elastase in congenital and cyclic neutropenia."Blood 96:2317-2322(2000) [PubMed] [Europe PMC] [Abstract]
  23. "Mutations in the ELA2 gene encoding neutrophil elastase are present in most patients with sporadic severe congenital neutropenia but only in some patients with the familial form of the disease."Blood 98:2645-2650(2001) [PubMed] [Europe PMC] [Abstract]
  24. "Paternal mosaicism proves the pathogenic nature of mutations in neutrophil elastase in severe congenital neutropenia."Blood 100:707-709(2002) [PubMed] [Europe PMC] [Abstract]
  25. "Mutations in the ELA2 gene correlate with more severe expression of neutropenia: a study of 81 patients from the French Neutropenia Register."Blood 103:4119-4125(2004) [PubMed] [Europe PMC] [Abstract]
  26. "Double de novo mutations of ELA2 in cyclic and severe congenital neutropenia."Hum. Mutat. 28:874-881(2007) [PubMed] [Europe PMC] [Abstract]
  27. "A novel mutation Ala57Val of the ELA2 gene in a Korean boy with severe congenital neutropenia."Ann. Hematol. 88:593-595(2009) [PubMed] [Europe PMC] [Abstract]
  28. "Homozygous HAX1 mutations in severe congenital neutropenia patients with sporadic disease: a novel mutation in two unrelated British kindreds."Br. J. Haematol. 144:762-770(2009) [PubMed] [Europe PMC] [Abstract]
  29. "Ela2 mutations and clinical manifestations in familial congenital neutropenia."J. Pediatr. Hematol. Oncol. 31:319-324(2009) [PubMed] [Europe PMC] [Abstract]
  30. "Digenic mutations in severe congenital neutropenia."Haematologica 95:1207-1210(2010) [PubMed] [Europe PMC] [Abstract]
  31. "Pegfilgrastim in children with severe congenital neutropenia."Pediatr. Blood Cancer 54:465-467(2010) [PubMed] [Europe PMC] [Abstract]
  32. "Severe congenital neutropenia in a multigenerational family with a novel neutrophil elastase (ELANE) mutation."Ann. Hematol. 90:151-158(2011) [PubMed] [Europe PMC] [Abstract]
  33. "Four novel ELANE mutations in patients with congenital neutropenia."Pediatr. Blood Cancer 57:332-335(2011) [PubMed] [Europe PMC] [Abstract]
  34. "The spectrum of ELANE mutations and their implications in severe congenital and cyclic neutropenia."Hum. Mutat. 34:905-914(2013) [PubMed] [Europe PMC] [Abstract]