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Centromere Protein E (CENPE)

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CENP-E; KIF10; Centromere-Associated Protein E

Centromere Protein E (CENPE)
CCC A276
HGNC 1856
MGI 1098230
PubMed 22110139
RGD 1307115
UniProt Q02224
Wiki CENPE

CENP-E is a large protein of ∼312 kDa consisting of an NH2-terminal kinesin motor domain tethered to a globular COOH-terminal domain via an extended “rod” domain predicted to form a coiled coil. Kinesin motors hydrolyze ATP to produce directed mechanical force along microtubules. In the case of CENP-E, the motor activity is involved in establishing and maintaining stable connections between mitotic chromosomes and the microtubules of the mitotic spindle. CENP-E exhibits a periodic accumulation and loss, with maximal levels found during late G2 and M-phases of the cell cycle, and minimum levels in early G1. CENP-E is thus appropriately spatially and temporally positioned to integrate the regulation of the mitotic checkpoint with the mechanics of chromosome-microtubule interactions.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA276Hu01 Recombinant Centromere Protein E (CENPE) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA276Hu01 Polyclonal Antibody to Centromere Protein E (CENPE) WB; IHC; ICC; IP.
MAA276Hu21 Monoclonal Antibody to Centromere Protein E (CENPE) WB; IHC; ICC; IP.
Assay Kits SEA276Hu ELISA Kit for Centromere Protein E (CENPE) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Centromere Protein E (CENPE) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Centromere Protein E (CENPE) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Centromere Protein E (CENPE) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Centromere Protein E (CENPE) CLIA Kit Customized Service Offer
n/a ELISA Kit for Centromere Protein E (CENPE) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA276Ra01 Recombinant Centromere Protein E (CENPE) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA276Ra01 Polyclonal Antibody to Centromere Protein E (CENPE) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Centromere Protein E (CENPE) CLIA Kit Customized Service Offer
n/a ELISA Kit for Centromere Protein E (CENPE) ELISA Kit Customized Service Offer
  1. "CENP-E is a putative kinetochore motor that accumulates just before mitosis."Nature 359:536-539(1992) [PubMed] [Europe PMC] [Abstract]
  2. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Mitotic HeLa cells contain a CENP-E-associated minus end-directed microtubule motor."EMBO J. 14:918-926(1995) [PubMed] [Europe PMC] [Abstract]
  5. "Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1."J. Cell Biol. 143:49-63(1998) [PubMed] [Europe PMC] [Abstract]
  6. "Farnesyl transferase inhibitors block the farnesylation of CENP-E and CENP-F and alter the association of CENP-E with the microtubules."J. Biol. Chem. 275:30451-30457(2000) [PubMed] [Europe PMC] [Abstract]
  7. "Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation."EMBO J. 23:3237-3248(2004) [PubMed] [Europe PMC] [Abstract]
  8. "Human NUF2 interacts with centromere-associated protein E and is essential for a stable spindle microtubule-kinetochore attachment."J. Biol. Chem. 282:21415-21424(2007) [PubMed] [Europe PMC] [Abstract]
  9. "Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry."Proteomics 7:868-874(2007) [PubMed] [Europe PMC] [Abstract]
  10. "Septin 7 interacts with centromere-associated protein E and is required for its kinetochore localization."J. Biol. Chem. 283:18916-18925(2008) [PubMed] [Europe PMC] [Abstract]
  11. "SUMO-2/3 modification and binding regulate the association of CENP-E with kinetochores and progression through mitosis."Mol. Cell 29:729-741(2008) [PubMed] [Europe PMC] [Abstract]
  12. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  13. "Defects in chromosome congression and mitotic progression in KIF18A-deficient cells are partly mediated through impaired functions of CENP-E."Cell Cycle 8:2643-2649(2009) [PubMed] [Europe PMC] [Abstract]
  14. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  15. "CENP-E kinesin interacts with SKAP protein to orchestrate accurate chromosome segregation in mitosis."J. Biol. Chem. 287:1500-1509(2012) [PubMed] [Europe PMC] [Abstract]
  16. "Mutations in CENPE define a novel kinetochore-centromeric mechanism for microcephalic primordial dwarfism."Hum. Genet. 133:1023-1039(2014) [PubMed] [Europe PMC] [Abstract]
  17. "Crystal structure of the motor domain of the human kinetochore protein CENP-E."J. Mol. Biol. 340:1107-1116(2004) [PubMed] [Europe PMC] [Abstract]