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Neutrophil Cytosolic Factor 4 (NCF4)

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NCF; P40PHOX; SH3PXD4; Neutrophil NADPH Oxidase Factor 4; SH3 and PX domain-containing protein 4

Neutrophil Cytosolic Factor 4 (NCF4)
CCC A328
HGNC 7662
MGI 109186
PubMed 23982929
RGD 1593157
UniProt Q15080
Wiki NCF4

Neutrophil cytosol factor 4 is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA328Hu01 Recombinant Neutrophil Cytosolic Factor 4 (NCF4) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA328Hu01 Polyclonal Antibody to Neutrophil Cytosolic Factor 4 (NCF4) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Neutrophil Cytosolic Factor 4 (NCF4) CLIA Kit Customized Service Offer
n/a ELISA Kit for Neutrophil Cytosolic Factor 4 (NCF4) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA328Mu01 Recombinant Neutrophil Cytosolic Factor 4 (NCF4) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA328Mu01 Polyclonal Antibody to Neutrophil Cytosolic Factor 4 (NCF4) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Neutrophil Cytosolic Factor 4 (NCF4) CLIA Kit Customized Service Offer
n/a ELISA Kit for Neutrophil Cytosolic Factor 4 (NCF4) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Neutrophil Cytosolic Factor 4 (NCF4) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Neutrophil Cytosolic Factor 4 (NCF4) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Neutrophil Cytosolic Factor 4 (NCF4) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Neutrophil Cytosolic Factor 4 (NCF4) CLIA Kit Customized Service Offer
n/a ELISA Kit for Neutrophil Cytosolic Factor 4 (NCF4) ELISA Kit Customized Service Offer
  1. "p40phox, a third cytosolic component of the activation complex of the NADPH oxidase to contain src homology 3 domains."Biochem. J. 296:557-561(1993) [PubMed] [Europe PMC] [Abstract]
  2. "Genomic structure, chromosomal localization, start of transcription, and tissue expression of the human p40-phox, a new component of the nicotinamide adenine dinucleotide phosphate-oxidase complex."Blood 88:2714-2721(1996) [PubMed] [Europe PMC] [Abstract]
  3. "Identification of a splice variant mRNA of p40phox, an NADPH oxidase component of phagocytes."FEBS Lett. 455:257-261(1999) [PubMed] [Europe PMC] [Abstract]
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  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  8. "p40(phox) is phosphorylated on threonine 154 and serine 315 during activation of the phagocyte NADPH oxidase. Implication of a protein kinase c-type kinase in the phosphorylation process."J. Biol. Chem. 273:30097-30103(1998) [PubMed] [Europe PMC] [Abstract]
  9. "The crystal structure of the PX domain from p40(phox) bound to phosphatidylinositol 3-phosphate."Mol. Cell 8:829-839(2001) [PubMed] [Europe PMC] [Abstract]
  10. "PB1 domain-mediated heterodimerization in NADPH oxidase and signaling complexes of atypical protein kinase C with Par6 and p62."Mol. Cell 12:39-50(2003) [PubMed] [Europe PMC] [Abstract]
  11. "Effects of p47phox C terminus phosphorylations on binding interactions with p40phox and p67phox. Structural and functional comparison of p40phox and p67phox SH3 domains."J. Biol. Chem. 280:13752-13761(2005) [PubMed] [Europe PMC] [Abstract]
  12. "Full-length p40phox structure suggests a basis for regulation mechanism of its membrane binding."EMBO J. 26:1176-1186(2007) [PubMed] [Europe PMC] [Abstract]
  13. "A new genetic subgroup of chronic granulomatous disease with autosomal recessive mutations in p40 phox and selective defects in neutrophil NADPH oxidase activity."Blood 114:3309-3315(2009) [PubMed] [Europe PMC] [Abstract]