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Protein Kinase C Iota (PKCi)

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PKC-I; PRKCI; PRKC-I; NPKC-Iota; Atypical protein kinase C-lambda/iota

Protein Kinase C Iota (PKCi)
CCC A495
HGNC 9404
MGI 99260
PubMed 15994303
RGD 620961
UniProt P41743
Wiki PRKCI

Protein Kinase C Iota is a protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol.

This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA495Hu01 Recombinant Protein Kinase C Iota (PKCi) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA495Hu01 Polyclonal Antibody to Protein Kinase C Iota (PKCi) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Protein Kinase C Iota (PKCi) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein Kinase C Iota (PKCi) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Protein Kinase C Iota (PKCi) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Protein Kinase C Iota (PKCi) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Protein Kinase C Iota (PKCi) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Protein Kinase C Iota (PKCi) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein Kinase C Iota (PKCi) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Protein Kinase C Iota (PKCi) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Protein Kinase C Iota (PKCi) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Protein Kinase C Iota (PKCi) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Protein Kinase C Iota (PKCi) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein Kinase C Iota (PKCi) ELISA Kit Customized Service Offer
  1. "Molecular cloning and characterization of PKC iota, an atypical isoform of protein kinase C derived from insulin-secreting cells."J. Biol. Chem. 268:24296-24302(1993) [PubMed] [Europe PMC] [Abstract]
  2. "Human protein kinase C iota gene (PRKCI) is closely linked to the BTK gene in Xq21.3."Genomics 26:629-631(1995) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Lambda-interacting protein, a novel protein that specifically interacts with the zinc finger domain of the atypical protein kinase C isotype lambda/iota and stimulates its kinase activity in vitro and in vivo."Mol. Cell. Biol. 16:105-114(1996) [PubMed] [Europe PMC] [Abstract]
  5. "Atypical protein kinase C iota protects human leukemia cells against drug-induced apoptosis."J. Biol. Chem. 272:27521-27524(1997) [PubMed] [Europe PMC] [Abstract]
  6. "Localization of atypical protein kinase C isoforms into lysosome-targeted endosomes through interaction with p62."Mol. Cell. Biol. 18:3069-3080(1998) [PubMed] [Europe PMC] [Abstract]
  7. "Overexpression of atypical PKC in PC12 cells enhances NGF-responsiveness and survival through an NF-kappaB dependent pathway."Cell Death Differ. 6:753-764(1999) [PubMed] [Europe PMC] [Abstract]
  8. "The interaction of p62 with RIP links the atypical PKCs to NF-kappaB activation."EMBO J. 18:3044-3053(1999) [PubMed] [Europe PMC] [Abstract]
  9. "Unique structural and functional properties of the ATP-binding domain of atypical protein kinase C-iota."J. Biol. Chem. 275:33289-33296(2000) [PubMed] [Europe PMC] [Abstract]
  10. "Protein kinase C iota protects neural cells against apoptosis induced by amyloid beta-peptide."Brain Res. Mol. Brain Res. 82:107-113(2000) [PubMed] [Europe PMC] [Abstract]
  11. "Human homologues of the Caenorhabditis elegans cell polarity protein PAR6 as an adaptor that links the small GTPases Rac and Cdc42 to atypical protein kinase C."Genes Cells 6:107-119(2001) [PubMed] [Europe PMC] [Abstract]
  12. "Atypical protein kinase C is involved in the evolutionarily conserved par protein complex and plays a critical role in establishing epithelia-specific junctional structures."J. Cell Biol. 152:1183-1196(2001) [PubMed] [Europe PMC] [Abstract]
  13. "Glyceraldehyde-3-phosphate dehydrogenase is phosphorylated by protein kinase Ciota /lambda and plays a role in microtubule dynamics in the early secretory pathway."J. Biol. Chem. 277:3334-3341(2002) [PubMed] [Europe PMC] [Abstract]
  14. "Nerve growth factor stimulates multisite tyrosine phosphorylation and activation of the atypical protein kinase C's via a src kinase pathway."Mol. Cell. Biol. 21:8414-8427(2001) [PubMed] [Europe PMC] [Abstract]
  15. "Phosphorylation of tyrosine 256 facilitates nuclear import of atypical protein kinase C."J. Cell. Biochem. 85:42-53(2002) [PubMed] [Europe PMC] [Abstract]
  16. "Centaurin-alpha(1) associates with and is phosphorylated by isoforms of protein kinase C."Biochem. Biophys. Res. Commun. 307:459-465(2003) [PubMed] [Europe PMC] [Abstract]
  17. "Mammalian Lgl forms a protein complex with PAR-6 and aPKC independently of PAR-3 to regulate epithelial cell polarity."Curr. Biol. 13:734-743(2003) [PubMed] [Europe PMC] [Abstract]
  18. "Atypical protein kinase C plays a critical role in protein transport from pre-Golgi intermediates."J. Biol. Chem. 278:38015-38021(2003) [PubMed] [Europe PMC] [Abstract]
  19. "Rab2 interacts directly with atypical protein kinase C (aPKC) iota/lambda and inhibits aPKCiota/lambda-dependent glyceraldehyde-3-phosphate dehydrogenase phosphorylation."J. Biol. Chem. 278:52524-52530(2003) [PubMed] [Europe PMC] [Abstract]
  20. "Regulation of interleukin receptor-associated kinase (IRAK) phosphorylation and signaling by iota protein kinase C."J. Biol. Chem. 279:4161-4165(2004) [PubMed] [Europe PMC] [Abstract]
  21. "Atypical protein kinase Ciota plays a critical role in human lung cancer cell growth and tumorigenicity."J. Biol. Chem. 280:31109-31115(2005) [PubMed] [Europe PMC] [Abstract]
  22. "Cyclin-dependent kinase activating kinase/Cdk7 co-localizes with PKC-iota in human glioma cells."Tissue Cell 37:53-58(2005) [PubMed] [Europe PMC] [Abstract]
  23. "Src-dependent protein kinase C iota/lambda (aPKCiota/lambda) tyrosine phosphorylation is required for aPKCiota/lambda association with Rab2 and glyceraldehyde-3-phosphate dehydrogenase on pre-Golgi intermediates."J. Biol. Chem. 281:8436-8442(2006) [PubMed] [Europe PMC] [Abstract]
  24. "Atypical protein kinase C (iota) activates ezrin in the apical domain of intestinal epithelial cells."J. Cell Sci. 121:644-654(2008) [PubMed] [Europe PMC] [Abstract]
  25. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
  26. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  27. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  28. "Protein kinase C iota mediates lipid-induced apoptosis of human coronary artery endothelial cells."Microvasc. Res. 78:40-44(2009) [PubMed] [Europe PMC] [Abstract]
  29. "Ect2 links the PKCiota-Par6alpha complex to Rac1 activation and cellular transformation."Oncogene 28:3597-3607(2009) [PubMed] [Europe PMC] [Abstract]
  30. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  31. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  32. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  33. "PKC-? promotes glioblastoma cell survival by phosphorylating and inhibiting BAD through a phosphatidylinositol 3-kinase pathway."Biochim. Biophys. Acta 1813:1190-1197(2011) [PubMed] [Europe PMC] [Abstract]
  34. "Oncogenic activity of Ect2 is regulated through protein kinase C iota-mediated phosphorylation."J. Biol. Chem. 286:8149-8157(2011) [PubMed] [Europe PMC] [Abstract]
  35. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  36. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  37. "Solution structure of atypical protein kinase C PB1 domain and its mode of interaction with ZIP/p62 and MEK5."J. Biol. Chem. 279:31883-31890(2004) [PubMed] [Europe PMC] [Abstract]
  38. "Structure of a cell polarity regulator, a complex between atypical PKC and Par6 PB1 domains."J. Biol. Chem. 280:9653-9661(2005) [PubMed] [Europe PMC] [Abstract]
  39. "Crystal structure of the catalytic domain of human atypical protein kinase C-iota reveals interaction mode of phosphorylation site in turn motif."J. Mol. Biol. 352:918-931(2005) [PubMed] [Europe PMC] [Abstract]
  40. "Patterns of somatic mutation in human cancer genomes." Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]