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Cyclin Dependent Kinase 9 (CDK9)

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C-2k; CDC2L4; PITALRE; TAK; CDC2-Related Kinase; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; Tat-associated kinase complex catalytic subunit

Cyclin Dependent Kinase 9 (CDK9)
CCC A496
HGNC 1780
MGI 1328368
PubMed 10958691
RGD 1359638
UniProt P50750
Wiki CDK9

CDK9 is a cyclin-dependent kinase associated with P-TEFb. The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA496Hu01 Recombinant Cyclin Dependent Kinase 9 (CDK9) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA496Hu01 Polyclonal Antibody to Cyclin Dependent Kinase 9 (CDK9) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Cyclin Dependent Kinase 9 (CDK9) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cyclin Dependent Kinase 9 (CDK9) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cyclin Dependent Kinase 9 (CDK9) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cyclin Dependent Kinase 9 (CDK9) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cyclin Dependent Kinase 9 (CDK9) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Cyclin Dependent Kinase 9 (CDK9) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cyclin Dependent Kinase 9 (CDK9) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cyclin Dependent Kinase 9 (CDK9) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cyclin Dependent Kinase 9 (CDK9) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cyclin Dependent Kinase 9 (CDK9) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Cyclin Dependent Kinase 9 (CDK9) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cyclin Dependent Kinase 9 (CDK9) ELISA Kit Customized Service Offer
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  11. "FACT relieves DSIF/NELF-mediated inhibition of transcriptional elongation and reveals functional differences between P-TEFb and TFIIH."Mol. Cell 5:1067-1072(2000) [PubMed] [Europe PMC] [Abstract]
  12. "Domains in the SPT5 protein that modulate its transcriptional regulatory properties."Mol. Cell. Biol. 20:2970-2983(2000) [PubMed] [Europe PMC] [Abstract]
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  22. "Methylation of SPT5 regulates its interaction with RNA polymerase II and transcriptional elongation properties."Mol. Cell 11:1055-1066(2003) [PubMed] [Europe PMC] [Abstract]
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  25. "Analysis of the large inactive P-TEFb complex indicates that it contains one 7SK molecule, a dimer of HEXIM1 or HEXIM2, and two P-TEFb molecules containing Cdk9 phosphorylated at threonine 186."J. Biol. Chem. 280:28819-28826(2005) [PubMed] [Europe PMC] [Abstract]
  26. "The bromodomain protein Brd4 is a positive regulatory component of P-TEFb and stimulates RNA polymerase II-dependent transcription."Mol. Cell 19:523-534(2005) [PubMed] [Europe PMC] [Abstract]
  27. "Recruitment of P-TEFb for stimulation of transcriptional elongation by the bromodomain protein Brd4."Mol. Cell 19:535-545(2005) [PubMed] [Europe PMC] [Abstract]
  28. "The functional role of an interleukin 6-inducible CDK9.STAT3 complex in human gamma-fibrinogen gene expression."J. Biol. Chem. 282:37091-37102(2007) [PubMed] [Europe PMC] [Abstract]
  29. "Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme."Mol. Cell 27:262-274(2007) [PubMed] [Europe PMC] [Abstract]
  30. "Regulation of P-TEFb elongation complex activity by CDK9 acetylation."Mol. Cell. Biol. 27:4641-4651(2007) [PubMed] [Europe PMC] [Abstract]
  31. "PP2B and PP1alpha cooperatively disrupt 7SK snRNP to release P-TEFb for transcription in response to Ca2+ signaling."Genes Dev. 22:1356-1368(2008) [PubMed] [Europe PMC] [Abstract]
  32. "Phosphatase PPM1A regulates phosphorylation of Thr-186 in the Cdk9 T-loop."J. Biol. Chem. 283:33578-33584(2008) [PubMed] [Europe PMC] [Abstract]
  33. "A La-related protein modulates 7SK snRNP integrity to suppress P-TEFb-dependent transcriptional elongation and tumorigenesis."Mol. Cell 29:588-599(2008) [PubMed] [Europe PMC] [Abstract]
  34. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  35. "Acetylation of conserved lysines in the catalytic core of cyclin-dependent kinase 9 inhibits kinase activity and regulates transcription."Mol. Cell. Biol. 28:2201-2212(2008) [PubMed] [Europe PMC] [Abstract]
  36. "RelA Ser276 phosphorylation is required for activation of a subset of NF-kappaB-dependent genes by recruiting cyclin-dependent kinase 9/cyclin T1 complexes."Mol. Cell. Biol. 28:3623-3638(2008) [PubMed] [Europe PMC] [Abstract]
  37. "Insights into the function of the human P-TEFb component CDK9 in the regulation of chromatin modifications and co-transcriptional mRNA processing."Cell Cycle 8:3636-3642(2009) [PubMed] [Europe PMC] [Abstract]
  38. "CDK9 directs H2B monoubiquitination and controls replication-dependent histone mRNA 3'-end processing."EMBO Rep. 10:894-900(2009) [PubMed] [Europe PMC] [Abstract]
  39. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  40. "55K isoform of CDK9 associates with Ku70 and is involved in DNA repair."Biochem. Biophys. Res. Commun. 397:245-250(2010) [PubMed] [Europe PMC] [Abstract]
  41. "Cyclin-dependent kinase 9-cyclin K functions in the replication stress response."EMBO Rep. 11:876-882(2010) [PubMed] [Europe PMC] [Abstract]
  42. "Cyclin-dependent kinase-9 is a component of the p300/GATA4 complex required for phenylephrine-induced hypertrophy in cardiomyocytes."J. Biol. Chem. 285:9556-9568(2010) [PubMed] [Europe PMC] [Abstract]
  43. "HIV-1 Tat and host AFF4 recruit two transcription elongation factors into a bifunctional complex for coordinated activation of HIV-1 transcription."Mol. Cell 38:428-438(2010) [PubMed] [Europe PMC] [Abstract]
  44. "AFF4, a component of the ELL/P-TEFb elongation complex and a shared subunit of MLL chimeras, can link transcription elongation to leukemia."Mol. Cell 37:429-437(2010) [PubMed] [Europe PMC] [Abstract]
  45. "CDK9 regulates AR promoter selectivity and cell growth through serine 81 phosphorylation."Mol. Endocrinol. 24:2267-2280(2010) [PubMed] [Europe PMC] [Abstract]
  46. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  47. "The little elongation complex regulates small nuclear RNA transcription."Mol. Cell 44:954-965(2011) [PubMed] [Europe PMC] [Abstract]
  48. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  49. "CDKI-71, a novel CDK9 inhibitor, is preferentially cytotoxic to cancer cells compared to flavopiridol."Int. J. Cancer 130:1216-1226(2012) [PubMed] [Europe PMC] [Abstract]
  50. "Transcription factor IIS cooperates with the E3 ligase UBR5 to ubiquitinate the CDK9 subunit of the positive transcription elongation factor B."J. Biol. Chem. 286:5012-5022(2011) [PubMed] [Europe PMC] [Abstract]
  51. "Cdk9 T-loop phosphorylation is regulated by the calcium signaling pathway."J. Cell. Physiol. 227:609-617(2012) [PubMed] [Europe PMC] [Abstract]
  52. "Protein phosphatase-1 activates CDK9 by dephosphorylating Ser175."PLoS ONE 6:E18985-E18985(2011) [PubMed] [Europe PMC] [Abstract]
  53. "Controlling the elongation phase of transcription with P-TEFb."Mol. Cell 23:297-305(2006) [PubMed] [Europe PMC] [Abstract]
  54. "Expanding role of cyclin dependent kinases in cytokine inducible gene expression."Cell Cycle 7:2661-2666(2008) [PubMed] [Europe PMC] [Abstract]
  55. "Role of the cyclin-dependent kinase 9-related pathway in mammalian gene expression and human diseases."Cell Cycle 7:3664-3668(2008) [PubMed] [Europe PMC] [Abstract]
  56. "Cyclin-dependent kinase 9: a key transcriptional regulator and potential drug target in oncology, virology and cardiology."Trends Pharmacol. Sci. 29:302-313(2008) [PubMed] [Europe PMC] [Abstract]
  57. "Cell cycle, CDKs and cancer: a changing paradigm."Nat. Rev. Cancer 9:153-166(2009) [PubMed] [Europe PMC] [Abstract]
  58. "Pharmacological targeting of CDK9 in cardiac hypertrophy."Med. Res. Rev. 30:646-666(2010) [PubMed] [Europe PMC] [Abstract]
  59. "A role for cdk9-cyclin k in maintaining genome integrity."Cell Cycle 10:28-32(2011) [PubMed] [Europe PMC] [Abstract]
  60. "Herpes simplex virus 1 ICP22 inhibits the transcription of viral gene promoters by binding to and blocking the recruitment of P-TEFb."PLoS ONE 7:E45749-E45749(2012) [PubMed] [Europe PMC] [Abstract]
  61. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  62. "Crystal structure of HIV-1 Tat complexed with human P-TEFb."Nature 465:747-751(2010) [PubMed] [Europe PMC] [Abstract]
  63. "The structure of P-TEFb (CDK9/cyclin T1), its complex with flavopiridol and regulation by phosphorylation."EMBO J. 27:1907-1918(2008) [PubMed] [Europe PMC] [Abstract]
  64. "Halogen bonds form the basis for selective P-TEFb inhibition by DRB."Chem. Biol. 17:931-936(2010) [PubMed] [Europe PMC] [Abstract]
  65. "CDK inhibitors roscovitine and CR8 trigger Mcl-1 down-regulation and apoptotic cell death in neuroblastoma cells."Genes Cancer 1:369-380(2010) [PubMed] [Europe PMC] [Abstract]
  66. "Patterns of somatic mutation in human cancer genomes." Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]