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Protein Kinase N1 (PKN1)

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PAK1; DBK; PRK1; PRKCL1; Protein Kinase C-Like 1; Protein kinase PKN-alpha; Protein-kinase C-related kinase 1; Serine-threonine protein kinase N

Protein Kinase N1 (PKN1)
CCC A501
HGNC 9405
MGI 108022
PubMed 24114839
RGD 69308
UniProt Q16512
Wiki PKN1

Serine/threonine-protein kinase N1 encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis.

The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA501Hu01 Recombinant Protein Kinase N1 (PKN1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA501Hu01 Polyclonal Antibody to Protein Kinase N1 (PKN1) WB; ICC; IHC-P; IHC-F; ELISA.
MAA501Hu21 Monoclonal Antibody to Protein Kinase N1 (PKN1) WB; IHC; ICC; IP.
Assay Kits SEA501Hu ELISA Kit for Protein Kinase N1 (PKN1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Protein Kinase N1 (PKN1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Protein Kinase N1 (PKN1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Protein Kinase N1 (PKN1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Protein Kinase N1 (PKN1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein Kinase N1 (PKN1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Protein Kinase N1 (PKN1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Protein Kinase N1 (PKN1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Protein Kinase N1 (PKN1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Protein Kinase N1 (PKN1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein Kinase N1 (PKN1) ELISA Kit Customized Service Offer
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  19. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
  20. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  21. "Phosphorylation of histone H3 at threonine 11 establishes a novel chromatin mark for transcriptional regulation."Nat. Cell Biol. 10:53-60(2008) [PubMed] [Europe PMC] [Abstract]
  22. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  23. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  24. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  25. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  26. "Protein kinase C-related kinase targets nuclear localization signals in a subset of class IIa histone deacetylases."FEBS Lett. 584:1103-1110(2010) [PubMed] [Europe PMC] [Abstract]
  27. "Phosphorylation of histone H3T6 by PKCbeta(I) controls demethylation at histone H3K4."Nature 464:792-796(2010) [PubMed] [Europe PMC] [Abstract]
  28. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  29. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  30. "Regulatory domain selectivity in the cell-type specific PKN-dependence of cell migration."PLoS ONE 6:E21732-E21732(2011) [PubMed] [Europe PMC] [Abstract]
  31. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  32. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  33. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  34. "The structural basis of Rho effector recognition revealed by the crystal structure of human RhoA complexed with the effector domain of PKN/PRK1."Mol. Cell 4:793-803(1999) [PubMed] [Europe PMC] [Abstract]
  35. "Biochemical and crystallographic characterization of a Rho effector domain of the protein serine/threonine kinase N in a complex with RhoA."J. Struct. Biol. 126:166-170(1999) [PubMed] [Europe PMC] [Abstract]
  36. "Molecular dissection of the interaction between the small G proteins Rac1 and RhoA and protein kinase C-related kinase 1 (PRK1)."J. Biol. Chem. 278:50578-50587(2003) [PubMed] [Europe PMC] [Abstract]
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  38. "Structure of an SspH1-PKN1 complex reveals the basis for host substrate recognition and mechanism of activation for a bacterial E3 ubiquitin ligase."Mol. Cell. Biol. 34:362-373(2014) [PubMed] [Europe PMC] [Abstract]
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