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Transcription Factor P65 (NFKB3)

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Nuclear factor NF-kappa-B p65 subunit; NFKBP65; RELA; p65; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3

Transcription Factor P65 (NFKB3)
CCC A616
HGNC 9955
MGI 103290
PubMed 16135789
RGD 727889
UniProt Q04206
Wiki RELA

The transcriptional coactivator CBP/p300 associates with through 2 sites, an N-terminal domain that interacts with the C-terminal region of unphosphorylated p65, and a second domain that only interacts with p65 phosphorylated on serine-276. Phosphorylation by PKA both weakens the interaction between the N- and C-terminal regions of p65 and creates an additional site for interaction with CBP/p300. Therefore, PKA regulates the transcriptional activity of NF-kappa-B by modulating its interaction with CBP/p300.

The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB ), which inactivate NFKB by trapping it in the cytoplasm.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA616Hu01 Recombinant Transcription Factor P65 (NFKB3) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA616Hu01 Polyclonal Antibody to Transcription Factor P65 (NFKB3) WB; IHC; ICC; IP.
MAA616Hu22 Monoclonal Antibody to Transcription Factor P65 (NFKB3) WB; IHC; ICC; IP.
Assay Kits SEA616Hu ELISA Kit for Transcription Factor P65 (NFKB3) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA616Mu01 Recombinant Transcription Factor P65 (NFKB3) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA616Mu01 Polyclonal Antibody to Transcription Factor P65 (NFKB3) WB; IHC; ICC; IP.
LAA616Mu71 Biotin-Linked Polyclonal Antibody to Transcription Factor P65 (NFKB3) WB; IHC; ICC.
LAA616Mu81 FITC-Linked Polyclonal Antibody to Transcription Factor P65 (NFKB3) WB; IHC; ICC; IF.
Assay Kits SEA616Mu ELISA Kit for Transcription Factor P65 (NFKB3) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA616Ra01 Recombinant Transcription Factor P65 (NFKB3) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA616Ra01 Polyclonal Antibody to Transcription Factor P65 (NFKB3) WB; IHC; ICC; IP.
MAA616Ra21 Monoclonal Antibody to Transcription Factor P65 (NFKB3) WB; IHC; ICC; IP.
Assay Kits SEA616Ra ELISA Kit for Transcription Factor P65 (NFKB3) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Danio rerio (Zebrafish)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA616Fi01 Recombinant Transcription Factor P65 (NFKB3) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies n/a Monoclonal Antibody to Transcription Factor P65 (NFKB3) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Transcription Factor P65 (NFKB3) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Transcription Factor P65 (NFKB3) CLIA Kit Customized Service Offer
n/a ELISA Kit for Transcription Factor P65 (NFKB3) ELISA Kit Customized Service Offer
  1. "Isolation of a rel-related human cDNA that potentially encodes the 65-kD subunit of NF-kappa B."Science 251:1490-1493(1991) [PubMed] [Europe PMC] [Abstract]
  2. "Genomic organization of the gene encoding the p65 subunit of NF-kappa B: multiple variants of the p65 protein may be generated by alternative splicing."Hum. Mol. Genet. 2:1895-1900(1993) [PubMed] [Europe PMC] [Abstract]
  3. "An alternatively spliced transcript, p65 delta 2, of the gene encoding the p65 subunit of the transcription factor NF-kappa B."Gene 138:265-266(1994) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Functional characterization of the NF-kappa B p65 transcriptional activator and an alternatively spliced derivative."Mol. Cell. Biol. 12:444-454(1992) [PubMed] [Europe PMC] [Abstract]
  6. "A novel complex between the p65 subunit of NF-kappa B and c-Rel binds to a DNA element involved in the phorbol ester induction of the human urokinase gene."EMBO J. 11:205-213(1992) [PubMed] [Europe PMC] [Abstract]
  7. "I kappa B/MAD-3 masks the nuclear localization signal of NF-kappa B p65 and requires the transactivation domain to inhibit NF-kappa B p65 DNA binding."Mol. Biol. Cell 3:1339-1352(1992) [PubMed] [Europe PMC] [Abstract]
  8. "Activation of multiple NF-kappa B/Rel DNA-binding complexes by tumor necrosis factor."Oncogene 9:1487-1492(1994) [PubMed] [Europe PMC] [Abstract]
  9. "Regulation of intercellular adhesion molecule-1 gene by tumor necrosis factor-alpha is mediated by the nuclear factor-kappaB heterodimers p65/p65 and p65/c-Rel in the absence of p50."Cell Growth Differ. 8:335-342(1997) [PubMed] [Europe PMC] [Abstract]
  10. "A new member of the IkappaB protein family, IkappaB epsilon, inhibits RelA (p65)-mediated NF-kappaB transcription."Mol. Cell. Biol. 17:6184-6190(1997) [PubMed] [Europe PMC] [Abstract]
  11. "IKAP is a scaffold protein of the IkappaB kinase complex."Nature 395:292-296(1998) [PubMed] [Europe PMC] [Abstract]
  12. "IkappaB kinases phosphorylate NF-kappaB p65 subunit on serine 536 in the transactivation domain."J. Biol. Chem. 274:30353-30356(1999) [PubMed] [Europe PMC] [Abstract]
  13. "NF-kappaB subunit p65 binds to 53BP2 and inhibits cell death induced by 53BP2."Oncogene 18:5177-5186(1999) [PubMed] [Europe PMC] [Abstract]
  14. "Yersinia enterocolitica invasin protein triggers IL-8 production in epithelial cells via activation of Rel p65-p65 homodimers."FASEB J. 14:1471-1484(2000) [PubMed] [Europe PMC] [Abstract]
  15. "Inhibition of nuclear factor-kappaB-mediated transcription by association with the amino-terminal enhancer of split, a Groucho-related protein lacking WD40 repeats."J. Biol. Chem. 275:4383-4390(2000) [PubMed] [Europe PMC] [Abstract]
  16. "Tumor necrosis factor alpha-induced phosphorylation of RelA/p65 on Ser529 is controlled by casein kinase II."J. Biol. Chem. 275:32592-32597(2000) [PubMed] [Europe PMC] [Abstract]
  17. "The tumor suppressor protein menin interacts with NF-kappaB proteins and inhibits NF-kappaB-mediated transactivation."Oncogene 20:4917-4925(2001) [PubMed] [Europe PMC] [Abstract]
  18. "Duration of nuclear NF-kappaB action regulated by reversible acetylation."Science 293:1653-1657(2001) [PubMed] [Europe PMC] [Abstract]
  19. "A novel protein overexpressed in hepatoma accelerates export of NF-kappa B from the nucleus and inhibits p53-dependent apoptosis."Cancer Cell 2:335-346(2002) [PubMed] [Europe PMC] [Abstract]
  20. "Acetylation of RelA at discrete sites regulates distinct nuclear functions of NF-kappaB."EMBO J. 21:6539-6548(2002) [PubMed] [Europe PMC] [Abstract]
  21. "The phosphorylation status of nuclear NF-kappa B determines its association with CBP/p300 or HDAC-1."Mol. Cell 9:625-636(2002) [PubMed] [Europe PMC] [Abstract]
  22. "Transcriptional activation of the NF-kappaB p65 subunit by mitogen- and stress-activated protein kinase-1 (MSK1)."EMBO J. 22:1313-1324(2003) [PubMed] [Europe PMC] [Abstract]
  23. "Post-activation turn-off of NF-kappa B-dependent transcription is regulated by acetylation of p65."J. Biol. Chem. 278:2758-2766(2003) [PubMed] [Europe PMC] [Abstract]
  24. "RING finger protein AO7 supports NF-kappaB-mediated transcription by interacting with the transactivation domain of the p65 subunit."J. Biol. Chem. 278:26879-26887(2003) [PubMed] [Europe PMC] [Abstract]
  25. "Glycogen synthase kinase-3 beta regulates NF-kappa B1/p105 stability."J. Biol. Chem. 278:39583-39590(2003) [PubMed] [Europe PMC] [Abstract]
  26. "Regulation of NF-kappaB signaling by Pin1-dependent prolyl isomerization and ubiquitin-mediated proteolysis of p65/RelA."Mol. Cell 12:1413-1426(2003) [PubMed] [Europe PMC] [Abstract]
  27. "Identification of a ZU5 and death domain-containing inhibitor of NF-kappaB."J. Biol. Chem. 279:17819-17825(2004) [PubMed] [Europe PMC] [Abstract]
  28. "Suppression of MEK/ERK signaling pathway enhances cisplatin-induced NF-kappaB activation by protein phosphatase 4-mediated NF-kappaB p65 Thr dephosphorylation."J. Biol. Chem. 279:26143-26148(2004) [PubMed] [Europe PMC] [Abstract]
  29. "Degradation of promoter-bound p65/RelA is essential for the prompt termination of the nuclear factor kappaB response."J. Exp. Med. 200:107-113(2004) [PubMed] [Europe PMC] [Abstract]
  30. "Identification of beta-arrestin2 as a G protein-coupled receptor-stimulated regulator of NF-kappaB pathways."Mol. Cell 14:303-317(2004) [PubMed] [Europe PMC] [Abstract]
  31. "The candidate tumour suppressor protein ING4 regulates brain tumour growth and angiogenesis."Nature 428:328-332(2004) [PubMed] [Europe PMC] [Abstract]
  32. "Regulation of NF-kappaB and p53 through activation of ATR and Chk1 by the ARF tumour suppressor."EMBO J. 24:1157-1169(2005) [PubMed] [Europe PMC] [Abstract]
  33. "IKKbeta phosphorylates p65 at S468 in transactivation domain 2."FASEB J. 19:1758-1760(2005) [PubMed] [Europe PMC] [Abstract]
  34. "cis-acting, element-specific transcriptional activity of differentially phosphorylated nuclear factor-kappa B."J. Biol. Chem. 280:244-252(2005) [PubMed] [Europe PMC] [Abstract]
  35. "COMMD proteins, a novel family of structural and functional homologs of MURR1."J. Biol. Chem. 280:22222-22232(2005) [PubMed] [Europe PMC] [Abstract]
  36. "NF-kappaB RelA phosphorylation regulates RelA acetylation."Mol. Cell. Biol. 25:7966-7975(2005) [PubMed] [Europe PMC] [Abstract]
  37. "Foxp3 interacts with nuclear factor of activated T cells and NF-kappa B to repress cytokine gene expression and effector functions of T helper cells."Proc. Natl. Acad. Sci. U.S.A. 102:5138-5143(2005) [PubMed] [Europe PMC] [Abstract]
  38. "Respiratory syncytial virus M2-1 protein induces the activation of nuclear factor kappa B."Virology 331:260-268(2005) [PubMed] [Europe PMC] [Abstract]
  39. "Activation of the nuclear factor kappaB pathway by astrocyte elevated gene-1: implications for tumor progression and metastasis."Cancer Res. 66:1509-1516(2006) [PubMed] [Europe PMC] [Abstract]
  40. "Human ubiquitin specific protease 31 is a deubiquitinating enzyme implicated in activation of nuclear factor-kappaB."Cell. Signal. 18:83-92(2006) [PubMed] [Europe PMC] [Abstract]
  41. "Inducible phosphorylation of NF-kappa B p65 at serine 468 by T cell costimulation is mediated by IKK epsilon."J. Biol. Chem. 281:6175-6183(2006) [PubMed] [Europe PMC] [Abstract]
  42. "Breast cancer metastasis suppressor 1 functions as a corepressor by enhancing histone deacetylase 1-mediated deacetylation of RelA/p65 and promoting apoptosis."Mol. Cell. Biol. 26:8683-8696(2006) [PubMed] [Europe PMC] [Abstract]
  43. "Coactivator-associated arginine methyltransferase-1 enhances nuclear factor-kappaB-mediated gene transcription through methylation of histone H3 at arginine 17."Mol. Endocrinol. 20:1562-1573(2006) [PubMed] [Europe PMC] [Abstract]
  44. "IL-1 receptor-associated kinase 1 is critical for latent membrane protein 1-induced p65/RelA serine 536 phosphorylation and NF-kappaB activation."Proc. Natl. Acad. Sci. U.S.A. 103:2689-2694(2006) [PubMed] [Europe PMC] [Abstract]
  45. "Nine-amino-acid transactivation domain: establishment and prediction utilities."Genomics 89:756-768(2007) [PubMed] [Europe PMC] [Abstract]
  46. "UXT is a novel and essential cofactor in the NF-kappaB transcriptional enhanceosome."J. Cell Biol. 178:231-244(2007) [PubMed] [Europe PMC] [Abstract]
  47. "The familial Mediterranean fever protein, pyrin, is cleaved by caspase-1 and activates NF-kappaB through its N-terminal fragment."Blood 112:1794-1803(2008) [PubMed] [Europe PMC] [Abstract]
  48. "Functional characterization of the atopy-associated gene PHF11."J. Allergy Clin. Immunol. 121:1148-1154(2008) [PubMed] [Europe PMC] [Abstract]
  49. "AKIP1 enhances NF-kappaB-dependent gene expression by promoting the nuclear retention and phosphorylation of p65."J. Biol. Chem. 283:7834-7843(2008) [PubMed] [Europe PMC] [Abstract]
  50. "The DEAD-box RNA helicase DDX1 interacts with RelA and enhances nuclear factor kappaB-mediated transcription."J. Cell. Biochem. 106:296-305(2009) [PubMed] [Europe PMC] [Abstract]
  51. "Brd4 coactivates transcriptional activation of NF-kappaB via specific binding to acetylated RelA."Mol. Cell. Biol. 29:1375-1387(2009) [PubMed] [Europe PMC] [Abstract]
  52. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  53. "A novel LZAP-binding protein, NLBP, inhibits cell invasion."J. Biol. Chem. 285:12232-12240(2010) [PubMed] [Europe PMC] [Abstract]
  54. "SIRT2 regulates NF-kappaB dependent gene expression through deacetylation of p65 Lys310."J. Cell Sci. 123:4251-4258(2010) [PubMed] [Europe PMC] [Abstract]
  55. "Zinc finger protein Gfi1 controls the endotoxin-mediated Toll-like receptor inflammatory response by antagonizing NF-kappaB p65."Mol. Cell. Biol. 30:3929-3942(2010) [PubMed] [Europe PMC] [Abstract]
  56. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  57. "NF-kappaB repression by PIAS3 mediated RelA SUMOylation."PLoS ONE 7:E37636-E37636(2012) [PubMed] [Europe PMC] [Abstract]
  58. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  59. "C11orf95-RELA fusions drive oncogenic NF-kappaB signalling in ependymoma." Nature 506:451-455(2014) [PubMed] [Europe PMC] [Abstract]
  60. "Structure of an IkappaBalpha/NF-kappaB complex."Cell 95:749-758(1998) [PubMed] [Europe PMC] [Abstract]