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Dickkopf Related Protein 1 (DKK1)

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SK; Dickkopf Homolog 1(Xenopus Laevis)

Dickkopf Related Protein 1 (DKK1)
CCC A741
HGNC 2891
MGI 1329040
PubMed 14695408
RGD 1307313
UniProt O94907
Wiki DKK1

Dickkopf-related protein 1 is a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Sequence analysis predicted that the 266-amino acid DKK1 protein contains a 31-amino acid N-terminal signal peptide, 2 clusters of cysteine residues, and a potential C-terminal N-glycosylation site. SDS-PAGE and Western blot analysis demonstrated that DKK1 is expressed as a 35-kD doublet protein, which is larger than the deduced molecular mass of 26 kD.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA741Hu01 Recombinant Dickkopf Related Protein 1 (DKK1) Positive Control; Immunogen; SDS-PAGE; WB.
APA741Hu01 Active Dickkopf Related Protein 1 (DKK1) Cell culture; Activity Assays.
EPA741Hu61 Eukaryotic Dickkopf Related Protein 1 (DKK1) Positive Control; Immunogen; SDS-PAGE; WB.
APA741Hu61 Active Dickkopf Related Protein 1 (DKK1) Cell culture; Activity Assays.
Antibodies PAA741Hu01 Polyclonal Antibody to Dickkopf Related Protein 1 (DKK1) WB; IHC; ICC; IP.
MAA741Hu22 Monoclonal Antibody to Dickkopf Related Protein 1 (DKK1) WB
MAA741Hu21 Monoclonal Antibody to Dickkopf Related Protein 1 (DKK1) WB; IHC; ICC; IP.
MAA741Hu23 Monoclonal Antibody to Dickkopf Related Protein 1 (DKK1) WB; IHC; ICC; IP.
MAA741Hu26 Monoclonal Antibody to Dickkopf Related Protein 1 (DKK1) WB; IHC; ICC; IP.
MAA741Hu29 Monoclonal Antibody to Dickkopf Related Protein 1 (DKK1) WB; IHC; ICC; IP.
MAA741Hu24 Monoclonal Antibody to Dickkopf Related Protein 1 (DKK1) WB; IHC; ICC; IP.
MAA741Hu25 Monoclonal Antibody to Dickkopf Related Protein 1 (DKK1) WB; IHC; ICC; IP.
MAA741Hu27 Monoclonal Antibody to Dickkopf Related Protein 1 (DKK1) WB; IHC; ICC; IP.
Assay Kits SEA741Hu ELISA Kit for Dickkopf Related Protein 1 (DKK1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA741Mu01 Recombinant Dickkopf Related Protein 1 (DKK1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA741Mu02 Recombinant Dickkopf Related Protein 1 (DKK1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA741Mu01 Polyclonal Antibody to Dickkopf Related Protein 1 (DKK1) WB; IHC; ICC; IP.
PAA741Mu02 Polyclonal Antibody to Dickkopf Related Protein 1 (DKK1) WB; IHC; ICC; IP.
Assay Kits SEA741Mu ELISA Kit for Dickkopf Related Protein 1 (DKK1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA741Ra01 Recombinant Dickkopf Related Protein 1 (DKK1) Positive Control; Immunogen; SDS-PAGE; WB.
APA741Ra01 Active Dickkopf Related Protein 1 (DKK1) Cell culture; Activity Assays.
Antibodies PAA741Ra01 Polyclonal Antibody to Dickkopf Related Protein 1 (DKK1) WB; IHC; ICC; IP.
LAA741Ra81 FITC-Linked Polyclonal Antibody to Dickkopf Related Protein 1 (DKK1) WB; IHC; ICC; IF.
LAA741Ra71 Biotin-Linked Polyclonal Antibody to Dickkopf Related Protein 1 (DKK1) WB; IHC; ICC.
MAA741Ra21 Monoclonal Antibody to Dickkopf Related Protein 1 (DKK1) WB; IHC; ICC; IP.
Assay Kits SEA741Ra ELISA Kit for Dickkopf Related Protein 1 (DKK1) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "Isolation and biochemical characterization of the human Dkk-1 homologue, a novel inhibitor of mammalian Wnt signaling."J. Biol. Chem. 274:19465-19472(1999) [PubMed] [Europe PMC] [Abstract]
  2. "Functional and structural diversity of the human Dickkopf gene family."Gene 238:301-313(1999) [PubMed] [Europe PMC] [Abstract]
  3. "The genomic structure, chromosome location, and analysis of the human DKK1 head inducer gene as a candidate for holoprosencephaly."Cytogenet. Cell Genet. 89:220-224(2000) [PubMed] [Europe PMC] [Abstract]
  4. "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
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  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Signal peptide prediction based on analysis of experimentally verified cleavage sites."Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
  8. "Head inducer Dickkopf-1 is a ligand for Wnt coreceptor LRP6."Curr. Biol. 11:951-961(2001) [PubMed] [Europe PMC] [Abstract]
  9. "Function and biological roles of the Dickkopf family of Wnt modulators."Oncogene 25:7469-7481(2006) [PubMed] [Europe PMC] [Abstract]
  10. "A cell-based Dkk1 binding assay reveals roles for extracellular domains of LRP5 in Dkk1 interaction and highlights differences between wild-type and the high bone mass mutant LRP5(G171V)."J. Cell. Biochem. 108:1066-1075(2009) [PubMed] [Europe PMC] [Abstract]
  11. "Reconstitution of a frizzled8.Wnt3a.LRP6 signaling complex reveals multiple Wnt and Dkk1 binding sites on LRP6."J. Biol. Chem. 285:9172-9179(2010) [PubMed] [Europe PMC] [Abstract]
  12. "Human Dickkopf-1 (huDKK1) protein: characterization of glycosylation and determination of disulfide linkages in the two cysteine-rich domains."Protein Sci. 20:1802-1813(2011) [PubMed] [Europe PMC] [Abstract]
  13. "Structural basis of Wnt signaling inhibition by Dickkopf binding to LRP5/6."Dev. Cell 21:862-873(2011) [PubMed] [Europe PMC] [Abstract]