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Apoptotic Peptidase Activating Factor 1 (APAF1)

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CED4; Apoptotic Protease Activating Factor

Apoptotic Peptidase Activating Factor 1 (APAF1)
CCC B054
HGNC 576
MGI 1306796
PubMed 15105372
RGD 620575
UniProt O14727
Wiki Apaf-1
Apoptotic peptidase activating factor 1 is a proteincontains several copies of the WD40 repeat domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. The precise mechanism for this reaction is still debated though work published by Guy Salvesen suggests that the apoptosome may induce caspase 9 dimerization and subsequent autocatalysis. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis.Alternative splicing results in several transcript variants encoding different isoforms.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB054Hu01 Recombinant Apoptotic Peptidase Activating Factor 1 (APAF1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB054Hu01 Polyclonal Antibody to Apoptotic Peptidase Activating Factor 1 (APAF1) WB; IHC; ICC; IP.
MAB054Hu22 Monoclonal Antibody to Apoptotic Peptidase Activating Factor 1 (APAF1) WB; IHC; ICC; IP.
Assay Kits SEB054Hu ELISA Kit for Apoptotic Peptidase Activating Factor 1 (APAF1) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB054Hu CLIA Kit for Apoptotic Peptidase Activating Factor 1 (APAF1) Chemiluminescent immunoassay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB054Mu01 Recombinant Apoptotic Peptidase Activating Factor 1 (APAF1) Positive Control; Immunogen; SDS-PAGE; WB.
RPB054Mu02 Recombinant Apoptotic Peptidase Activating Factor 1 (APAF1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB054Mu01 Polyclonal Antibody to Apoptotic Peptidase Activating Factor 1 (APAF1) WB; IHC; IF
PAB054Mu02 Polyclonal Antibody to Apoptotic Peptidase Activating Factor 1 (APAF1) WB; IHC; ICC; IP.
Assay Kits SCB054Mu CLIA Kit for Apoptotic Peptidase Activating Factor 1 (APAF1) Chemiluminescent immunoassay for Antigen Detection.
SEB054Mu ELISA Kit for Apoptotic Peptidase Activating Factor 1 (APAF1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB054Ra01 Recombinant Apoptotic Peptidase Activating Factor 1 (APAF1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB054Ra01 Polyclonal Antibody to Apoptotic Peptidase Activating Factor 1 (APAF1) WB; IHC; ICC; IP.
MAB054Ra21 Monoclonal Antibody to Apoptotic Peptidase Activating Factor 1 (APAF1) WB; IHC; ICC; IP.
MAB054Ra23 Monoclonal Antibody to Apoptotic Peptidase Activating Factor 1 (APAF1) WB; IHC; ICC; IP.
MAB054Ra22 Monoclonal Antibody to Apoptotic Peptidase Activating Factor 1 (APAF1) WB; IHC; ICC; IP.
Assay Kits SCB054Ra CLIA Kit for Apoptotic Peptidase Activating Factor 1 (APAF1) Chemiluminescent immunoassay for Antigen Detection.
SEB054Ra ELISA Kit for Apoptotic Peptidase Activating Factor 1 (APAF1) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3."Cell 90:405-413(1997) [PubMed] [Europe PMC] [Abstract]
  2. "Three new types of Apaf-1 in mammalian cells."Biochem. Biophys. Res. Commun. 261:746-749(1999) [PubMed] [Europe PMC] [Abstract]
  3. "Cytochrome c and dATP-mediated oligomerization of Apaf-1 is a prerequisite for procaspase-9 activation."J. Biol. Chem. 274:17941-17945(1999) [PubMed] [Europe PMC] [Abstract]
  4. "Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis."EMBO J. 18:3586-3595(1999) [PubMed] [Europe PMC] [Abstract]
  5. "APAF-1-ALT, a novel alternative splicing form of APAF-1, potentially causes impeded ability of undergoing DNA damage-induced apoptosis in the LNCaP human prostate cancer cell line."Biochem. Biophys. Res. Commun. 306:537-543(2003) [PubMed] [Europe PMC] [Abstract]
  6. "Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro."DNA Res. 4:307-313(1997) [PubMed] [Europe PMC] [Abstract]
  7. "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  9. "Autoactivation of procaspase-9 by Apaf-1-mediated oligomerization."Mol. Cell 1:949-957(1998) [PubMed] [Europe PMC] [Abstract]
  10. "Apaf-1 is a transcriptional target for E2F and p53."Nat. Cell Biol. 3:552-558(2001) [PubMed] [Europe PMC] [Abstract]
  11. "Induced inhibition of ischemic/hypoxic injury by APIP, a novel Apaf-1-interacting protein."J. Biol. Chem. 279:39942-39950(2004) [PubMed] [Europe PMC] [Abstract]
  12. "Calcium blocks formation of apoptosome by preventing nucleotide exchange in Apaf-1."Mol. Cell 25:181-192(2007) [PubMed] [Europe PMC] [Abstract]
  13. "Integrity of ATP binding site is essential for effective inhibition of the intrinsic apoptosis pathway by NAIP."Biochem. Biophys. Res. Commun. 407:158-162(2011) [PubMed] [Europe PMC] [Abstract]
  14. "Crystal structure of Apaf-1 caspase recruitment domain: an alpha-helical Greek key fold for apoptotic signaling."J. Mol. Biol. 293:439-447(1999) [PubMed] [Europe PMC] [Abstract]
  15. "Solution structure and mutagenesis of the caspase recruitment domain (CARD) from Apaf-1."Cell Death Differ. 6:1125-1132(1999) [PubMed] [Europe PMC] [Abstract]