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Centromere Protein B (CENPB)

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CENP-B; Major centromere autoantigen B

Centromere Protein B (CENPB)
CCC B125
HGNC 1852
MGI 88376
PubMed 1893793
RGD 1304794
UniProt P07199
Wiki CENPB

Centromere Protein B is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA.

This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB125Hu01 Recombinant Centromere Protein B (CENPB) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB125Hu01 Polyclonal Antibody to Centromere Protein B (CENPB) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Centromere Protein B (CENPB) CLIA Kit Customized Service Offer
n/a ELISA Kit for Centromere Protein B (CENPB) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Centromere Protein B (CENPB) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Centromere Protein B (CENPB) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Centromere Protein B (CENPB) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Centromere Protein B (CENPB) CLIA Kit Customized Service Offer
n/a ELISA Kit for Centromere Protein B (CENPB) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Centromere Protein B (CENPB) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Centromere Protein B (CENPB) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Centromere Protein B (CENPB) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Centromere Protein B (CENPB) CLIA Kit Customized Service Offer
n/a ELISA Kit for Centromere Protein B (CENPB) ELISA Kit Customized Service Offer
  1. "CENP-B is a highly conserved mammalian centromere protein with homology to the helix-loop-helix family of proteins."Chromosoma 100:360-370(1991) [PubMed] [Europe PMC] [Abstract]
  2. "The DNA sequence and comparative analysis of human chromosome 20." Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Molecular cloning of cDNA for CENP-B, the major human centromere autoantigen."J. Cell Biol. 104:817-829(1987) [PubMed] [Europe PMC] [Abstract]
  5. "A human centromere protein, CENP-B, has a DNA binding domain containing four potential alpha helices at the NH2 terminus, which is separable from dimerizing activity."J. Cell Biol. 119:1413-1427(1992) [PubMed] [Europe PMC] [Abstract]
  6. "Assembly of the inner kinetochore proteins CENP-A and CENP-B in living human cells."ChemBioChem 9:77-92(2008) [PubMed] [Europe PMC] [Abstract]
  7. "Live-cell imaging reveals sustained centromere binding of CENP-T via CENP-A and CENP-B."J. Biophotonics 1:245-254(2008) [PubMed] [Europe PMC] [Abstract]
  8. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
  9. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  10. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  11. "Identification of novel alpha-n-methylation of CENP-B that regulates its binding to the centromeric DNA."J. Proteome Res. 12:4167-4175(2013) [PubMed] [Europe PMC] [Abstract]
  12. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  13. "A helix-turn-helix structure unit in human centromere protein B (CENP-B)."EMBO J. 17:827-837(1998) [PubMed] [Europe PMC] [Abstract]
  14. "Crystal structure of the CENP-B protein-DNA complex: the DNA-binding domains of CENP-B induce kinks in the CENP-B box DNA."EMBO J. 20:6612-6618(2001) [PubMed] [Europe PMC] [Abstract]
  15. "Crystal structure of the human centromere protein B (CENP-B) dimerization domain at 1.65-A resolution."J. Biol. Chem. 278:51454-51461(2003) [PubMed] [Europe PMC] [Abstract]