≡

Clusterin (CLU)

[Edit]

CLU; TRPM2; KUB1; CLI; APOJ; SGP2; AAG4; APO-J; SP-40; Apolipoprotein J; Complement Lysis Inhibitor; 40,Sulfated Glycoprotein 2; Testosterone-Repressed Prostate Message 2

Clusterin (CLU)
CCC B180
HGNC 2095
MGI 88423
PubMed 11906815
RGD 3907
UniProt P10909
Wiki Clusterin

Clusterin (apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis. This protein has several synonyms: dimeric acidic glycoprotein (DAG protein), testosterone repressed prostate message-2 (TRPM-2), sulfated glycoprotein-2 (SGP-2) and complement lysis inhibitor (CLI).This protein was first identified in 1988 in seminal fluid.In humans the gene is encoded on chromosome 8 (8p21) and is highly conserved between species (70-80% homology).It is expressed in most mammalian tissues and can be found in blood plasma, milk, urine, cerebrospinal fluid and semen. A number of proteins have been found to affect its expression including Egr-1, members from the AP-1 complex, HSF1/2, Cdx1/2 and B-Myb.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB180Hu01 Recombinant Clusterin (CLU) Positive Control; Immunogen; SDS-PAGE; WB.
RPB180Hu02 Recombinant Clusterin (CLU) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB180Hu01 Polyclonal Antibody to Clusterin (CLU) WB; IHC
MAB180Hu22 Monoclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IP.
LAB180Hu71 Biotin-Linked Polyclonal Antibody to Clusterin (CLU) WB; IHC; ICC.
LAB180Hu81 FITC-Linked Polyclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IF.
MAB180Hu21 Monoclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IP.
MAB180Hu23 Monoclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IP.
Assay Kits SEB180Hu ELISA Kit for Clusterin (CLU) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB180Mu01 Recombinant Clusterin (CLU) Positive Control; Immunogen; SDS-PAGE; WB.
RPB180Mu02 Recombinant Clusterin (CLU) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB180Mu01 Polyclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IP.
LAB180Mu71 Biotin-Linked Polyclonal Antibody to Clusterin (CLU) WB; IHC; ICC.
LAB180Mu81 FITC-Linked Polyclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IF.
PAB180Mu02 Polyclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IP.
Assay Kits SEB180Mu ELISA Kit for Clusterin (CLU) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB180Ra01 Recombinant Clusterin (CLU) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB180Ra01 Polyclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IP.
LAB180Ra71 Biotin-Linked Polyclonal Antibody to Clusterin (CLU) WB; IHC; ICC.
LAB180Ra81 FITC-Linked Polyclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IF.
MAB180Ra21 Monoclonal Antibody to Clusterin (CLU) WB,IHC
Assay Kits SEB180Ra ELISA Kit for Clusterin (CLU) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Oryctolagus cuniculus (Rabbit)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB180Rb01 Recombinant Clusterin (CLU) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB180Rb51 Polyclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IP.
LAB180Rb71 Biotin-Linked Polyclonal Antibody to Clusterin (CLU) WB; IHC; ICC.
LAB180Rb81 FITC-Linked Polyclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IF.
MAB180Rb21 Monoclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IP.
PAB180Rb01 Polyclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IP.
MAB180Rb22 Monoclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IP.
MAB180Rb23 Monoclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IP.
MAB180Rb24 Monoclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Clusterin (CLU) CLIA Kit Customized Service Offer
n/a ELISA Kit for Clusterin (CLU) ELISA Kit Customized Service Offer

Organism species: Canis familiaris; Canine (Dog)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB180Ca01 Recombinant Clusterin (CLU) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB180Ca01 Polyclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IP.
LAB180Ca71 Biotin-Linked Polyclonal Antibody to Clusterin (CLU) WB; IHC; ICC.
LAB180Ca81 FITC-Linked Polyclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IF.
Assay Kits SEB180Ca ELISA Kit for Clusterin (CLU) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Sus scrofa; Porcine (Pig)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB180Po01 Recombinant Clusterin (CLU) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB180Po01 Polyclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IP.
LAB180Po71 Biotin-Linked Polyclonal Antibody to Clusterin (CLU) WB; IHC; ICC.
MAB180Po21 Monoclonal Antibody to Clusterin (CLU) WB,IHC
MAB180Po22 Monoclonal Antibody to Clusterin (CLU) WB; IHC.
MAB180Po23 Monoclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IP.
MAB180Po24 Monoclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IP.
MAB180Po25 Monoclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IP.
MAB180Po27 Monoclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IP.
MAB180Po29 Monoclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IP.
Assay Kits SEB180Po ELISA Kit for Clusterin (CLU) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Bos taurus; Bovine (Cattle)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB180Bo01 Recombinant Clusterin (CLU) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB180Bo01 Polyclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Clusterin (CLU) CLIA Kit Customized Service Offer
n/a ELISA Kit for Clusterin (CLU) ELISA Kit Customized Service Offer

Organism species: Ovis aries; Ovine (Sheep)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB180Ov01 Recombinant Clusterin (CLU) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB180Ov01 Polyclonal Antibody to Clusterin (CLU) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Clusterin (CLU) CLIA Kit Customized Service Offer
n/a ELISA Kit for Clusterin (CLU) ELISA Kit Customized Service Offer
  1. "Molecular structure and functional characterization of a human complement cytolysis inhibitor found in blood and seminal plasma: identity to sulfated glycoprotein 2, a constituent of rat testis fluid."Proc. Natl. Acad. Sci. U.S.A. 86:7123-7127(1989) [PubMed] [Europe PMC] [Abstract]
  2. "Molecular characterization of human TRPM-2/clusterin, a gene associated with sperm maturation, apoptosis and neurodegeneration."Eur. J. Biochem. 221:917-925(1994) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The full-ORF clone resource of the German cDNA consortium."BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
  5. "DNA sequence and analysis of human chromosome 8." Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Characterization of a human high density lipoprotein-associated protein, NA1/NA2. Identity with SP-40,40, an inhibitor of complement-mediated cytolysis."Arterioscler. Thromb. 11:645-652(1991) [PubMed] [Europe PMC] [Abstract]
  8. "Purification and characterization of apolipoprotein J."J. Biol. Chem. 265:14292-14297(1990) [PubMed] [Europe PMC] [Abstract]
  9. "The cerebrospinal-fluid soluble form of Alzheimer's amyloid beta is complexed to SP-40,40 (apolipoprotein J), an inhibitor of the complement membrane-attack complex."Biochem. J. 293:27-30(1993) [PubMed] [Europe PMC] [Abstract]
  10. "A serum protein SP40,40 modulates the formation of membrane attack complex of complement on erythrocytes."Mol. Immunol. 26:835-840(1989) [PubMed] [Europe PMC] [Abstract]
  11. "HDL particle associated proteins in plasma and cerebrospinal fluid: identification and partial sequencing."Appl. Theor. Electrophor. 1:73-76(1988) [PubMed] [Europe PMC] [Abstract]
  12. "Apolipoprotein J: structure and tissue distribution."Biochemistry 29:5380-5389(1990) [PubMed] [Europe PMC] [Abstract]
  13. "Human gliomas and epileptic foci express high levels of a mRNA related to rat testicular sulfated glycoprotein 2, a purported marker of cell death."Proc. Natl. Acad. Sci. U.S.A. 88:8577-8581(1991) [PubMed] [Europe PMC] [Abstract]
  14. "Molecular cloning and characterization of the novel, human complement-associated protein, SP-40,40: a link between the complement and reproductive systems."EMBO J. 8:711-718(1989) [PubMed] [Europe PMC] [Abstract]
  15. "SP-40,40, a protein involved in the control of the complement pathway, possesses a unique array of disulphide bridges."FEBS Lett. 297:70-76(1992) [PubMed] [Europe PMC] [Abstract]
  16. "The apolipoprotein A-I binding protein of placenta and the SP-40,40 protein of human blood are different proteins which both bind to apolipoprotein A-I."Biochim. Biophys. Acta 1086:255-260(1991) [PubMed] [Europe PMC] [Abstract]
  17. "Identification of the disulfide bonds in human plasma protein SP-40,40 (apolipoprotein-J)."J. Biochem. 112:557-561(1992) [PubMed] [Europe PMC] [Abstract]
  18. "Apolipoprotein J is associated with paraoxonase in human plasma."Biochemistry 33:832-839(1994) [PubMed] [Europe PMC] [Abstract]
  19. "Identification and characterization of glycosylation sites in human serum clusterin."Protein Sci. 6:2120-2133(1997) [PubMed] [Europe PMC] [Abstract]
  20. "Clusterin is an ATP-independent chaperone with very broad substrate specificity that stabilizes stressed proteins in a folding-competent state."Biochemistry 39:15953-15960(2000) [PubMed] [Europe PMC] [Abstract]
  21. "Suppression of apolipoprotein C-II amyloid formation by the extracellular chaperone, clusterin."Eur. J. Biochem. 269:2789-2794(2002) [PubMed] [Europe PMC] [Abstract]
  22. "Mildly acidic pH activates the extracellular molecular chaperone clusterin."J. Biol. Chem. 277:39532-39540(2002) [PubMed] [Europe PMC] [Abstract]
  23. "Synthesis and functional analyses of nuclear clusterin, a cell death protein."J. Biol. Chem. 278:11590-11600(2003) [PubMed] [Europe PMC] [Abstract]
  24. "Essential requirement of apolipoprotein J (clusterin) signaling for IkappaB expression and regulation of NF-kappaB activity."J. Biol. Chem. 278:38214-38219(2003) [PubMed] [Europe PMC] [Abstract]
  25. "Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry."Nat. Biotechnol. 21:660-666(2003) [PubMed] [Europe PMC] [Abstract]
  26. "Isolation and characterization of a novel gene CLUAP1 whose expression is frequently upregulated in colon cancer."Oncogene 23:9289-9294(2004) [PubMed] [Europe PMC] [Abstract]
  27. "Screening for N-glycosylated proteins by liquid chromatography mass spectrometry."Proteomics 4:454-465(2004) [PubMed] [Europe PMC] [Abstract]
  28. "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
  29. "Clusterin inhibits apoptosis by interacting with activated Bax."Nat. Cell Biol. 7:909-915(2005) [PubMed] [Europe PMC] [Abstract]
  30. "Transcriptomic and proteomic analyses of rhabdomyosarcoma cells reveal differential cellular gene expression in response to enterovirus 71 infection."Cell. Microbiol. 8:565-580(2006) [PubMed] [Europe PMC] [Abstract]
  31. "Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry."J. Proteome Res. 5:1493-1503(2006) [PubMed] [Europe PMC] [Abstract]
  32. "Elucidation of N-glycosylation sites on human platelet proteins: a glycoproteomic approach."Mol. Cell. Proteomics 5:226-233(2006) [PubMed] [Europe PMC] [Abstract]
  33. "Effects of glycosylation on the structure and function of the extracellular chaperone clusterin."Biochemistry 46:1412-1422(2007) [PubMed] [Europe PMC] [Abstract]
  34. "Multiple pathways regulating the anti-apoptotic protein clusterin in breast cancer."Biochim. Biophys. Acta 1772:1103-1111(2007) [PubMed] [Europe PMC] [Abstract]
  35. "Characterization of an eppin protein complex from human semen and spermatozoa."Biol. Reprod. 77:476-484(2007) [PubMed] [Europe PMC] [Abstract]
  36. "The extracellular chaperone clusterin influences amyloid formation and toxicity by interacting with prefibrillar structures."FASEB J. 21:2312-2322(2007) [PubMed] [Europe PMC] [Abstract]
  37. "Differential regulation of clusterin and its isoforms by androgens in prostate cells."J. Biol. Chem. 282:2278-2287(2007) [PubMed] [Europe PMC] [Abstract]
  38. "The extracellular chaperone clusterin potently inhibits human lysozyme amyloid formation by interacting with prefibrillar species."J. Mol. Biol. 369:157-167(2007) [PubMed] [Europe PMC] [Abstract]
  39. "Clusterin expression in normal mucosa and colorectal cancer."Mol. Cell. Proteomics 6:1039-1048(2007) [PubMed] [Europe PMC] [Abstract]
  40. "Stress-induced retrotranslocation of clusterin/ApoJ into the cytosol."Traffic 8:554-565(2007) [PubMed] [Europe PMC] [Abstract]
  41. "Identification of N-linked glycoproteins in human milk by hydrophilic interaction liquid chromatography and mass spectrometry."Proteomics 8:3833-3847(2008) [PubMed] [Europe PMC] [Abstract]
  42. "Structural characterization of clusterin-chaperone client protein complexes."J. Biol. Chem. 284:21920-21927(2009) [PubMed] [Europe PMC] [Abstract]
  43. "Clusterin is a short half-life, poly-ubiquitinated protein, which controls the fate of prostate cancer cells."J. Cell. Physiol. 219:314-323(2009) [PubMed] [Europe PMC] [Abstract]
  44. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
  45. "A strategy for precise and large scale identification of core fucosylated glycoproteins."Mol. Cell. Proteomics 8:913-923(2009) [PubMed] [Europe PMC] [Abstract]
  46. "Enrichment of glycopeptides for glycan structure and attachment site identification."Nat. Methods 6:809-811(2009) [PubMed] [Europe PMC] [Abstract]
  47. "Identification of human plasma proteins as major clients for the extracellular chaperone clusterin."J. Biol. Chem. 285:3532-3539(2010) [PubMed] [Europe PMC] [Abstract]
  48. "Clusterin facilitates COMMD1 and I-kappaB degradation to enhance NF-kappaB activity in prostate cancer cells."Mol. Cancer Res. 8:119-130(2010) [PubMed] [Europe PMC] [Abstract]
  49. "Clusterin facilitates in vivo clearance of extracellular misfolded proteins."Cell. Mol. Life Sci. 68:3919-3931(2011) [PubMed] [Europe PMC] [Abstract]
  50. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]