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ATP Dependent DNA ligase I (LIG1)

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Polydeoxyribonucleotide synthase [ATP] 1

ATP Dependent DNA ligase I (LIG1)
CCC B269
HGNC 6598
MGI 101789
PubMed 11414704
RGD 621424
UniProt P18858
Wiki LIG1
The major DNA ligase activity in proliferating mammalian cells is due to the high molecular weight enzyme designated DNA ligase I. Barnes et al. (1990) cloned cDNA for human DNA ligase I, both by functional complementation of a Saccharomyces cerevisiae cdc9 mutation and by hybridization with oligonucleotide probes corresponding to peptide sequences of the purified bovine enzyme. Hybridization between the cloned sequences and mRNA as well as genomic DNA indicated that the human enzyme is transcribed from a single-copy gene.
Barnes et al. (1990) mapped the DNA ligase I gene to chromosome 19 by hybridization to DNAs from well-characterized rodent-human somatic cell hybrids retaining subsets of human chromosomes.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB269Hu01 Recombinant ATP Dependent DNA ligase I (LIG1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB269Hu01 Polyclonal Antibody to ATP Dependent DNA ligase I (LIG1) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for ATP Dependent DNA ligase I (LIG1) CLIA Kit Customized Service Offer
n/a ELISA Kit for ATP Dependent DNA ligase I (LIG1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant ATP Dependent DNA ligase I (LIG1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to ATP Dependent DNA ligase I (LIG1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to ATP Dependent DNA ligase I (LIG1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for ATP Dependent DNA ligase I (LIG1) CLIA Kit Customized Service Offer
n/a ELISA Kit for ATP Dependent DNA ligase I (LIG1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB269Ra01 Recombinant ATP Dependent DNA ligase I (LIG1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB269Ra01 Polyclonal Antibody to ATP Dependent DNA ligase I (LIG1) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for ATP Dependent DNA ligase I (LIG1) CLIA Kit Customized Service Offer
n/a ELISA Kit for ATP Dependent DNA ligase I (LIG1) ELISA Kit Customized Service Offer
  1. "Human DNA ligase I cDNA: cloning and functional expression in Saccharomyces cerevisiae."Proc. Natl. Acad. Sci. U.S.A. 87:6679-6683(1990) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The DNA sequence and biology of human chromosome 19." Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "A wild-type DNA ligase I gene is expressed in Bloom's syndrome cells."Proc. Natl. Acad. Sci. U.S.A. 88:7615-7619(1991) [PubMed] [Europe PMC] [Abstract]
  6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  7. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  8. "Quantitative phosphoproteome profiling of Wnt3a-mediated signaling network: indicating the involvement of ribonucleoside-diphosphate reductase M2 subunit phosphorylation at residue serine 20 in canonical Wnt signal transduction."Mol. Cell. Proteomics 6:1952-1967(2007) [PubMed] [Europe PMC] [Abstract]
  9. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  10. "Proteomic analysis of ubiquitinated proteins in normal hepatocyte cell line Chang liver cells."Proteomics 8:2885-2896(2008) [PubMed] [Europe PMC] [Abstract]
  11. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  12. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  13. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  14. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  15. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  16. "Hypomorphic PCNA mutation underlies a human DNA repair disorder." J. Clin. Invest. 124:3137-3146(2014) [PubMed] [Europe PMC] [Abstract]
  17. "Human DNA ligase I completely encircles and partially unwinds nicked DNA."Nature 432:473-478(2004) [PubMed] [Europe PMC] [Abstract]
  18. "Mutations in the DNA ligase I gene of an individual with immunodeficiencies and cellular hypersensitivity to DNA-damaging agents."Cell 69:495-503(1992) [PubMed] [Europe PMC] [Abstract]
  19. "The consensus coding sequences of human breast and colorectal cancers." Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]