≡

Apoptosis Signal Regulating Kinase 1 (ASK1)

[Edit]

MAP3-K5; MAP3K5; ASK1; MAPKKK5; MEKK5; Mitogen-Activated Protein Kinase Kinase Kinase 5; MAPK/ERK kinase kinase 5

Apoptosis Signal Regulating Kinase 1 (ASK1)
CCC B358
HGNC 6857
MGI 1346876
PubMed 9774977
UniProt Q99683
Wiki ASK1

  • Apoptosis signal-regulating kinase 1 is an enzyme which in humans is encoded by the MAP3K5 gene. This gene is located on chromosome 6 at locus 6q22.33 and the transcribed protein contains 1,374 amino acids with 11 kinase subdomains. The ASK1 (MAPKKK5) protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; ASK1 does not activate MAPK/ERK.ASK1 found in the inactive form, bound to reduced thioredoxin.

  • When oxidized by a reactive oxygen species, Trx dissociates from ASK1. The ASK1, which is found as a homo-oligodimer, autophosphorylates and becomes an active MAP kinase kinase kinase.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB358Hu01 Recombinant Apoptosis Signal Regulating Kinase 1 (ASK1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB358Hu01 Polyclonal Antibody to Apoptosis Signal Regulating Kinase 1 (ASK1) WB; IHC; ICC; IP.
MAB358Hu22 Monoclonal Antibody to Apoptosis Signal Regulating Kinase 1 (ASK1) WB; IHC; ICC; IP.
Assay Kits SEB358Hu ELISA Kit for Apoptosis Signal Regulating Kinase 1 (ASK1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB358Mu01 Recombinant Apoptosis Signal Regulating Kinase 1 (ASK1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB358Mu01 Polyclonal Antibody to Apoptosis Signal Regulating Kinase 1 (ASK1) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Apoptosis Signal Regulating Kinase 1 (ASK1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Apoptosis Signal Regulating Kinase 1 (ASK1) ELISA Kit Customized Service Offer
  1. "Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase."J. Biol. Chem. 271:31607-31611(1996) [PubMed] [Europe PMC] [Abstract]
  2. "Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways."Science 275:90-94(1997) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The DNA sequence and analysis of human chromosome 6." Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Mammalian thioredoxin is a direct inhibitor of apoptosis signal-regulating kinase (ASK) 1."EMBO J. 17:2596-2606(1998) [PubMed] [Europe PMC] [Abstract]
  7. "ASK1 is essential for JNK/SAPK activation by TRAF2."Mol. Cell 2:389-395(1998) [PubMed] [Europe PMC] [Abstract]
  8. "Activation of apoptosis signal-regulating kinase 1 (ASK1) by the adapter protein Daxx."Science 281:1860-1863(1998) [PubMed] [Europe PMC] [Abstract]
  9. "Suppression of apoptosis signal-regulating kinase 1-induced cell death by 14-3-3 proteins."Proc. Natl. Acad. Sci. U.S.A. 96:8511-8515(1999) [PubMed] [Europe PMC] [Abstract]
  10. "Execution of apoptosis signal-regulating kinase 1 (ASK1)-induced apoptosis by the mitochondria-dependent caspase activation."J. Biol. Chem. 275:26576-26581(2000) [PubMed] [Europe PMC] [Abstract]
  11. "Activation of apoptosis signal-regulating kinase 1 (ASK1) by tumor necrosis factor receptor-associated factor 2 requires prior dissociation of the ASK1 inhibitor thioredoxin."Mol. Cell. Biol. 20:2198-2208(2000) [PubMed] [Europe PMC] [Abstract]
  12. "Beta-arrestin 2: a receptor-regulated MAPK scaffold for the activation of JNK3."Science 290:1574-1577(2000) [PubMed] [Europe PMC] [Abstract]
  13. "Negative feedback regulation of ASK1 by protein phosphatase 5 (PP5) in response to oxidative stress."EMBO J. 20:6028-6036(2001) [PubMed] [Europe PMC] [Abstract]
  14. "Apoptosis signal-regulating kinase 1 (ASK1) is an intracellular inducer of keratinocyte differentiation."J. Biol. Chem. 276:999-1004(2001) [PubMed] [Europe PMC] [Abstract]
  15. "Akt phosphorylates and negatively regulates apoptosis signal-regulating kinase 1."Mol. Cell. Biol. 21:893-901(2001) [PubMed] [Europe PMC] [Abstract]
  16. "HIV-1 Nef inhibits ASK1-dependent death signalling providing a potential mechanism for protecting the infected host cell."Nature 410:834-838(2001) [PubMed] [Europe PMC] [Abstract]
  17. "Raf-1 promotes cell survival by antagonizing apoptosis signal-regulating kinase 1 through a MEK-ERK independent mechanism."Proc. Natl. Acad. Sci. U.S.A. 98:7783-7788(2001) [PubMed] [Europe PMC] [Abstract]
  18. "Involvement of ASK1 in Ca2+-induced p38 MAP kinase activation."EMBO Rep. 5:161-166(2004) [PubMed] [Europe PMC] [Abstract]
  19. "Activation of apoptosis signal-regulating kinase 1 by the stress-induced activating phosphorylation of pre-formed oligomer."J. Cell. Physiol. 191:95-104(2002) [PubMed] [Europe PMC] [Abstract]
  20. "Type 1 insulin-like growth factor receptor (IGF-IR) signaling inhibits apoptosis signal-regulating kinase 1 (ASK1)."J. Biol. Chem. 278:13325-13332(2003) [PubMed] [Europe PMC] [Abstract]
  21. "AKT2 inhibition of cisplatin-induced JNK/p38 and Bax activation by phosphorylation of ASK1: implication of AKT2 in chemoresistance."J. Biol. Chem. 278:23432-23440(2003) [PubMed] [Europe PMC] [Abstract]
  22. "AIP1 mediates TNF-alpha-induced ASK1 activation by facilitating dissociation of ASK1 from its inhibitor 14-3-3."J. Clin. Invest. 111:1933-1943(2003) [PubMed] [Europe PMC] [Abstract]
  23. "Interaction of apoptosis signal-regulating kinase 1 with isoforms of 14-3-3 proteins."Exp. Cell Res. 294:581-591(2004) [PubMed] [Europe PMC] [Abstract]
  24. "Activation of apoptosis signal-regulating kinase 1 by reactive oxygen species through dephosphorylation at serine 967 and 14-3-3 dissociation."J. Biol. Chem. 279:10442-10449(2004) [PubMed] [Europe PMC] [Abstract]
  25. "AIP1/DAB2IP, a novel member of the Ras-GAP family, transduces TRAF2-induced ASK1-JNK activation."J. Biol. Chem. 279:44955-44965(2004) [PubMed] [Europe PMC] [Abstract]
  26. "Negative control of apoptosis signal-regulating kinase 1 through phosphorylation of Ser-1034."Oncogene 23:5099-5104(2004) [PubMed] [Europe PMC] [Abstract]
  27. "C-terminus of heat shock protein 70-interacting protein facilitates degradation of apoptosis signal-regulating kinase 1 and inhibits apoptosis signal-regulating kinase 1-dependent apoptosis."Cell Stress Chaperones 10:147-156(2005) [PubMed] [Europe PMC] [Abstract]
  28. "Tumor necrosis factor alpha-induced desumoylation and cytoplasmic translocation of homeodomain-interacting protein kinase 1 are critical for apoptosis signal-regulating kinase 1-JNK/p38 activation."J. Biol. Chem. 280:15061-15070(2005) [PubMed] [Europe PMC] [Abstract]
  29. "Recruitment of tumor necrosis factor receptor-associated factor family proteins to apoptosis signal-regulating kinase 1 signalosome is essential for oxidative stress-induced cell death."J. Biol. Chem. 280:37033-37040(2005) [PubMed] [Europe PMC] [Abstract]
  30. "SOCS1 inhibits tumor necrosis factor-induced activation of ASK1-JNK inflammatory signaling by mediating ASK1 degradation."J. Biol. Chem. 281:5559-5566(2006) [PubMed] [Europe PMC] [Abstract]
  31. "Regulation of apoptosis signal-regulating kinase 1 by protein phosphatase 2Cepsilon."Biochem. J. 405:591-596(2007) [PubMed] [Europe PMC] [Abstract]
  32. "Apoptosis signal-regulating kinase (ASK) 2 functions as a mitogen-activated protein kinase kinase kinase in a heteromeric complex with ASK1."J. Biol. Chem. 282:7522-7531(2007) [PubMed] [Europe PMC] [Abstract]
  33. "Tumor necrosis factor receptor 2 signaling induces selective c-IAP1-dependent ASK1 ubiquitination and terminates mitogen-activated protein kinase signaling."J. Biol. Chem. 282:7777-7782(2007) [PubMed] [Europe PMC] [Abstract]
  34. "RIP1-mediated AIP1 phosphorylation at a 14-3-3-binding site is critical for tumor necrosis factor-induced ASK1-JNK/p38 activation."J. Biol. Chem. 282:14788-14796(2007) [PubMed] [Europe PMC] [Abstract]
  35. "Thioredoxin and TRAF family proteins regulate reactive oxygen species-dependent activation of ASK1 through reciprocal modulation of the N-terminal homophilic interaction of ASK1."Mol. Cell. Biol. 27:8152-8163(2007) [PubMed] [Europe PMC] [Abstract]
  36. "The beta-arrestin-2 scaffold protein promotes c-Jun N-terminal kinase-3 activation by binding to its nonconserved N terminus."J. Biol. Chem. 283:15903-15911(2008) [PubMed] [Europe PMC] [Abstract]
  37. "Murine protein serine/threonine kinase 38 activates apoptosis signal-regulating kinase 1 via Thr 838 phosphorylation."J. Biol. Chem. 283:34541-34553(2008) [PubMed] [Europe PMC] [Abstract]
  38. "Phosphoproteome of resting human platelets."J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
  39. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  40. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  41. "PIM1 phosphorylates and negatively regulates ASK1-mediated apoptosis."Oncogene 28:4261-4271(2009) [PubMed] [Europe PMC] [Abstract]
  42. "Mitochondrial phosphoglycerate mutase 5 uses alternate catalytic activity as a protein serine/threonine phosphatase to activate ASK1."Proc. Natl. Acad. Sci. U.S.A. 106:12301-12305(2009) [PubMed] [Europe PMC] [Abstract]
  43. "Apoptosis signal-regulating kinase 1 in stress and immune response."Annu. Rev. Pharmacol. Toxicol. 48:199-225(2008) [PubMed] [Europe PMC] [Abstract]
  44. "The roles of ASK family proteins in stress responses and diseases."Cell Commun. Signal. 7:9-9(2009) [PubMed] [Europe PMC] [Abstract]
  45. "Positive regulation of apoptosis signal-regulating kinase 1 by dual-specificity phosphatase 13A."Cell. Mol. Life Sci. 67:2619-2629(2010) [PubMed] [Europe PMC] [Abstract]
  46. "The Kelch repeat protein KLHDC10 regulates oxidative stress-induced ASK1 activation by suppressing PP5."Mol. Cell 48:692-704(2012) [PubMed] [Europe PMC] [Abstract]
  47. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  48. "Structural and functional characterization of the human protein kinase ASK1."Structure 15:1215-1226(2007) [PubMed] [Europe PMC] [Abstract]
  49. "Patterns of somatic mutation in human cancer genomes." Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]