≡

MAP Kinase Activated Protein Kinase 2 (MAPKAPK2)

[Edit]

MK2; MAPK-activated protein kinase 2; Mitogen Activated Protein Kinase Activated Protein Kinase 2

MAP Kinase Activated Protein Kinase 2 (MAPKAPK2)
CCC B460
HGNC 6887
MGI 109298
PubMed 22883146
RGD 631362
UniProt P49137
Wiki MAPKAPK2

MAP kinase-activated protein kinase 2 is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation.

Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.The cDNA sequence revealed the following features (in 5-prime to 3-prime order): a proline-rich region containing 2 putative SH3-binding sites, a kinase catalytic domain, a threonine residue phosphorylated by MAP kinase, and a nuclear localization signal.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB460Hu01 Recombinant MAP Kinase Activated Protein Kinase 2 (MAPKAPK2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB460Hu01 Polyclonal Antibody to MAP Kinase Activated Protein Kinase 2 (MAPKAPK2) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for MAP Kinase Activated Protein Kinase 2 (MAPKAPK2) CLIA Kit Customized Service Offer
n/a ELISA Kit for MAP Kinase Activated Protein Kinase 2 (MAPKAPK2) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB460Mu01 Recombinant MAP Kinase Activated Protein Kinase 2 (MAPKAPK2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB460Mu01 Polyclonal Antibody to MAP Kinase Activated Protein Kinase 2 (MAPKAPK2) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for MAP Kinase Activated Protein Kinase 2 (MAPKAPK2) CLIA Kit Customized Service Offer
n/a ELISA Kit for MAP Kinase Activated Protein Kinase 2 (MAPKAPK2) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant MAP Kinase Activated Protein Kinase 2 (MAPKAPK2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to MAP Kinase Activated Protein Kinase 2 (MAPKAPK2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to MAP Kinase Activated Protein Kinase 2 (MAPKAPK2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for MAP Kinase Activated Protein Kinase 2 (MAPKAPK2) CLIA Kit Customized Service Offer
n/a ELISA Kit for MAP Kinase Activated Protein Kinase 2 (MAPKAPK2) ELISA Kit Customized Service Offer
  1. "The primary structure of a human MAP kinase activated protein kinase 2."Biochem. Biophys. Res. Commun. 200:1118-1124(1994) [PubMed] [Europe PMC] [Abstract]
  2. "The DNA sequence and biological annotation of human chromosome 1." Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The substrate specificity and structure of mitogen-activated protein (MAP) kinase-activated protein kinase-2."Biochem. J. 296:843-849(1993) [PubMed] [Europe PMC] [Abstract]
  5. "Small heat shock proteins are molecular chaperones."J. Biol. Chem. 268:1517-1520(1993) [PubMed] [Europe PMC] [Abstract]
  6. "Identification of novel phosphorylation sites required for activation of MAPKAP kinase-2."EMBO J. 14:5920-5930(1995) [PubMed] [Europe PMC] [Abstract]
  7. "A comparison of the substrate specificity of MAPKAP kinase-2 and MAPKAP kinase-3 and their activation by cytokines and cellular stress."FEBS Lett. 392:209-214(1996) [PubMed] [Europe PMC] [Abstract]
  8. "Regulation of Hsp27 oligomerization, chaperone function, and protective activity against oxidative stress/tumor necrosis factor alpha by phosphorylation."J. Biol. Chem. 274:18947-18956(1999) [PubMed] [Europe PMC] [Abstract]
  9. "Inhibition of SAPK2a/p38 prevents hnRNP A0 phosphorylation by MAPKAP-K2 and its interaction with cytokine mRNAs."EMBO J. 21:6505-6514(2002) [PubMed] [Europe PMC] [Abstract]
  10. "Arachidonic acid promotes phosphorylation of 5-lipoxygenase at Ser-271 by MAPK-activated protein kinase 2 (MK2)."J. Biol. Chem. 277:14793-14800(2002) [PubMed] [Europe PMC] [Abstract]
  11. "Affinity purification of ARE-binding proteins identifies polyA-binding protein 1 as a potential substrate in MK2-induced mRNA stabilization."Biochem. Biophys. Res. Commun. 301:665-670(2003) [PubMed] [Europe PMC] [Abstract]
  12. "MAPK-activated protein kinase-2 participates in p38 MAPK-dependent and ERK-dependent functions in human neutrophils."Cell. Signal. 15:993-1001(2003) [PubMed] [Europe PMC] [Abstract]
  13. "Stabilization of urokinase and urokinase receptor mRNAs by HuR is linked to its cytoplasmic accumulation induced by activated mitogen-activated protein kinase-activated protein kinase 2."Mol. Cell. Biol. 23:7177-7188(2003) [PubMed] [Europe PMC] [Abstract]
  14. "MK2-induced tristetraprolin:14-3-3 complexes prevent stress granule association and ARE-mRNA decay."EMBO J. 23:1313-1324(2004) [PubMed] [Europe PMC] [Abstract]
  15. "P66(ShcA) interacts with MAPKAP kinase 2 and regulates its activity."FEBS Lett. 564:205-211(2004) [PubMed] [Europe PMC] [Abstract]
  16. "MAPKAP kinase-2 is a cell cycle checkpoint kinase that regulates the G2/M transition and S phase progression in response to UV irradiation."Mol. Cell 17:37-48(2005) [PubMed] [Europe PMC] [Abstract]
  17. "MAPKAPK-2-mediated LIM-kinase activation is critical for VEGF-induced actin remodeling and cell migration."EMBO J. 25:713-726(2006) [PubMed] [Europe PMC] [Abstract]
  18. "Phosphorylation of HSF1 by MAPK-activated protein kinase 2 on serine 121, inhibits transcriptional activity and promotes HSP90 binding."J. Biol. Chem. 281:782-791(2006) [PubMed] [Europe PMC] [Abstract]
  19. "MAPKAPK2-mediated LSP1 phosphorylation and FMLP-induced neutrophil polarization."Biochem. Biophys. Res. Commun. 358:170-175(2007) [PubMed] [Europe PMC] [Abstract]
  20. "Roles for TAB1 in regulating the IL-1-dependent phosphorylation of the TAB3 regulatory subunit and activity of the TAK1 complex."Biochem. J. 409:711-722(2008) [PubMed] [Europe PMC] [Abstract]
  21. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  22. "DNA damage activates a spatially distinct late cytoplasmic cell-cycle checkpoint network controlled by MK2-mediated RNA stabilization."Mol. Cell 40:34-49(2010) [PubMed] [Europe PMC] [Abstract]
  23. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  24. "MK2 SUMOylation regulates actin filament remodeling and subsequent migration in endothelial cells by inhibiting MK2 kinase and HSP27 phosphorylation."Blood 117:2527-2537(2011) [PubMed] [Europe PMC] [Abstract]
  25. "MK2 and MK3--a pair of isoenzymes?"Front. Biosci. 13:5511-5521(2008) [PubMed] [Europe PMC] [Abstract]
  26. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  27. "Structure of mitogen-activated protein kinase-activated protein (MAPKAP) kinase 2 suggests a bifunctional switch that couples kinase activation with nuclear export."J. Biol. Chem. 277:37401-37405(2002) [PubMed] [Europe PMC] [Abstract]
  28. "Catalytically active MAP KAP kinase 2 structures in complex with staurosporine and ADP reveal differences with the autoinhibited enzyme."Structure 11:627-636(2003) [PubMed] [Europe PMC] [Abstract]
  29. "The discovery of carboline analogs as potent MAPKAP-K2 inhibitors." Bioorg. Med. Chem. Lett. 17:4664-4669(2007) [PubMed] [Europe PMC] [Abstract]
  30. "Crystal structure of the p38 alpha-MAPKAP kinase 2 heterodimer."J. Biol. Chem. 282:9733-9739(2007) [PubMed] [Europe PMC] [Abstract]
  31. ErratumJ. Biol. Chem. 282:14684-14684(2007)
  32. "Pyrrolopyridine inhibitors of mitogen-activated protein kinase-activated protein kinase 2 (MK-2)."J. Med. Chem. 50:2647-2654(2007) [PubMed] [Europe PMC] [Abstract]
  33. "Structural basis for a high affinity inhibitor bound to protein kinase MK2."J. Mol. Biol. 369:735-745(2007) [PubMed] [Europe PMC] [Abstract]
  34. "Molecular basis of MAPK-activated protein kinase 2:p38 assembly."Proc. Natl. Acad. Sci. U.S.A. 104:6353-6358(2007) [PubMed] [Europe PMC] [Abstract]
  35. "Patterns of somatic mutation in human cancer genomes." Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]