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Interleukin 1 Receptor Associated Kinase 4 (IRAK4)

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NY-REN-64

Interleukin 1 Receptor Associated Kinase 4 (IRAK4)
CCC B518
HGNC 17967
MGI 2182474
PubMed 23695305
RGD 1305303
UniProt Q9NWZ3
Wiki IRAK4

IRAK-4 is a protein kinase involved in signaling innate immune responses from Toll-like receptors. It also supports signaling from T-cell receptors.Animals without IRAK-4 are more susceptible to viruses and bacteria but completely resistant to LPS challenge.Unlike the other IRAK proteins, however, but similar to Pelle, IRAK4 has a short C-terminal domain. Northern blot analysis revealed expression of 3.0- and 4.4-kb transcripts, with strongest expression in kidney and liver. Luciferase reporter analysis showed that IRAK4 lacking the kinase domain inhibited IL1- but not tumor necrosis factor (TNF)- induced NFKB and IRAK1 activation. SDS-PAGE and autoradiographic analysis indicated that IRAK4 phosphorylates and activates IRAK1 at thr387, but not vice versa.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB518Hu01 Recombinant Interleukin 1 Receptor Associated Kinase 4 (IRAK4) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB518Hu01 Polyclonal Antibody to Interleukin 1 Receptor Associated Kinase 4 (IRAK4) WB; ICC; IHC-P; IHC-F; ELISA.
MAB518Hu22 Monoclonal Antibody to Interleukin 1 Receptor Associated Kinase 4 (IRAK4) WB; IHC; ICC; IP.
Assay Kits SEB518Hu ELISA Kit for Interleukin 1 Receptor Associated Kinase 4 (IRAK4) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Interleukin 1 Receptor Associated Kinase 4 (IRAK4) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Interleukin 1 Receptor Associated Kinase 4 (IRAK4) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Interleukin 1 Receptor Associated Kinase 4 (IRAK4) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Interleukin 1 Receptor Associated Kinase 4 (IRAK4) CLIA Kit Customized Service Offer
n/a ELISA Kit for Interleukin 1 Receptor Associated Kinase 4 (IRAK4) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB518Ra01 Recombinant Interleukin 1 Receptor Associated Kinase 4 (IRAK4) Positive Control; Immunogen; SDS-PAGE; WB.
RPB518Ra02 Recombinant Interleukin 1 Receptor Associated Kinase 4 (IRAK4) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB518Ra01 Polyclonal Antibody to Interleukin 1 Receptor Associated Kinase 4 (IRAK4) WB; IHC; ICC; IP.
MAB518Ra21 Monoclonal Antibody to Interleukin 1 Receptor Associated Kinase 4 (IRAK4) WB; IHC; ICC; IP.
Assay Kits SEB518Ra ELISA Kit for Interleukin 1 Receptor Associated Kinase 4 (IRAK4) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "IRAK4: a novel member of the IRAK family with the properties of an IRAK-kinase."Proc. Natl. Acad. Sci. U.S.A. 99:5567-5572(2002) [PubMed] [Europe PMC] [Abstract]
  2. "Antigens recognized by autologous antibody in patients with renal-cell carcinoma."Int. J. Cancer 83:456-464(1999) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The finished DNA sequence of human chromosome 12." Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Pellino 1 is required for interleukin-1 (IL-1)-mediated signaling through its interaction with the IL-1 receptor-associated kinase 4 (IRAK4)-IRAK-tumor necrosis factor receptor-associated factor 6 (TRAF6) complex."J. Biol. Chem. 278:10952-10956(2003) [PubMed] [Europe PMC] [Abstract]
  7. "Inhibition of interleukin 1 receptor/Toll-like receptor signaling through the alternatively spliced, short form of MyD88 is due to its failure to recruit IRAK-4."J. Exp. Med. 197:263-268(2003) [PubMed] [Europe PMC] [Abstract]
  8. "Distinct mutations in IRAK-4 confer hyporesponsiveness to lipopolysaccharide and interleukin-1 in a patient with recurrent bacterial infections."J. Exp. Med. 198:521-531(2003) [PubMed] [Europe PMC] [Abstract]
  9. "IRAK4 kinase activity is redundant for interleukin-1 (IL-1) receptor-associated kinase phosphorylation and IL-1 responsiveness."J. Biol. Chem. 279:26748-26753(2004) [PubMed] [Europe PMC] [Abstract]
  10. "Pyogenic bacterial infections in humans with IRAK-4 deficiency." Science 299:2076-2079(2003) [PubMed] [Europe PMC] [Abstract]
  11. "IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST 2 and induces T helper type 2-associated cytokines."Immunity 23:479-490(2005) [PubMed] [Europe PMC] [Abstract]
  12. "Smad6 negatively regulates interleukin 1-receptor-Toll-like receptor signaling through direct interaction with the adaptor Pellino-1."Nat. Immunol. 7:1057-1065(2006) [PubMed] [Europe PMC] [Abstract]
  13. "Cross-talk between IRAK-4 and the NADPH oxidase."Biochem. J. 403:451-461(2007) [PubMed] [Europe PMC] [Abstract]
  14. "IRAK-4 kinase activity is required for interleukin-1 (IL-1) receptor- and toll-like receptor 7-mediated signaling and gene expression."J. Biol. Chem. 282:13552-13560(2007) [PubMed] [Europe PMC] [Abstract]
  15. "IRAK4 and NEMO mutations in otherwise healthy children with recurrent invasive pneumococcal disease."J. Med. Genet. 44:16-23(2007) [PubMed] [Europe PMC] [Abstract]
  16. "The IRAK-catalysed activation of the E3 ligase function of Pellino isoforms induces the Lys63-linked polyubiquitination of IRAK1."Biochem. J. 409:43-52(2008) [PubMed] [Europe PMC] [Abstract]
  17. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  18. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  19. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  20. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  21. "IRAK1 and IRAK4 promote phosphorylation, ubiquitination, and degradation of MyD88 adaptor-like (Mal)."J. Biol. Chem. 285:18276-18282(2010) [PubMed] [Europe PMC] [Abstract]
  22. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  23. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  24. "Natural loss-of-function mutation of myeloid differentiation protein 88 disrupts its ability to form Myddosomes."J. Biol. Chem. 286:11875-11882(2011) [PubMed] [Europe PMC] [Abstract]
  25. "What the Myddosome structure tells us about the initiation of innate immunity."Trends Immunol. 32:104-109(2011) [PubMed] [Europe PMC] [Abstract]
  26. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  27. "Crystal structures of IRAK-4 kinase in complex with inhibitors: a serine/threonine kinase with tyrosine as a gatekeeper."Structure 14:1835-1844(2006) [PubMed] [Europe PMC] [Abstract]
  28. "Crystal structures of the apo and inhibited IRAK4 kinase domain."Submitted (DEC-2006) to the PDB data bank
  29. "Cutting Edge: IL-1 receptor-associated kinase 4 structures reveal novel features and multiple conformations."J. Immunol. 178:2641-2645(2007) [PubMed] [Europe PMC] [Abstract]
  30. "Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling."Nature 465:885-890(2010) [PubMed] [Europe PMC] [Abstract]
  31. "TLR9 activation induces normal neutrophil responses in a child with IRAK-4 deficiency: involvement of the direct PI3K pathway."J. Immunol. 179:4754-4765(2007) [PubMed] [Europe PMC] [Abstract]
  32. "Patterns of somatic mutation in human cancer genomes." Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
  33. "Impaired neutrophil migration and phagocytosis in IRAK-4 deficiency."Br. J. Haematol. 147:153-156(2009) [PubMed] [Europe PMC] [Abstract]
  34. "Clinical features and outcome of patients with IRAK-4 and MyD88 deficiency." Medicine (Baltimore) 89:403-425(2010) [PubMed] [Europe PMC] [Abstract]
  35. "Functional assessment of the mutational effects of human IRAK4 and MyD88 genes."Mol. Immunol. 58:66-76(2014) [PubMed] [Europe PMC] [Abstract]