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Band 3 (BND3)

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CD233; SLC4A1; SLC4-A1; WD; AE1; DI; EMPB3; EPB3; FR; RTA1A; SW; WD1; WR; Anion Exchange Protein 1; Solute Carrier Family 4,Anion Exchanger,Member 1; Erythrocyte Membrane Protein Band 3

Band 3 (BND3)
CCC B681
HGNC 11027
MGI 109393
PubMed 21455263
RGD 3710
UniProt P02730
Wiki CD233

  • Anion Exchanger 1  is a phylogenetically preserved transport protein responsible for mediating the electroneutral exchange of chloride (Cl-) for bicarbonate (HCO3-) across a plasma membrane.It is ubiquitous throughout the vertebrates. In humans it is present in two specific sites: the erythrocyte (red blood cell) cell membrane and the basolateral surface of the alpha-intercalated cell (the acid secreting cell type) in the collecting duct of the kidney. The erythrocyte and kidney forms are different isoforms of the same protein.AE1 was discovered following SDS-PAGE gel electrophoresis of erythrocyte cell membrane. The large 'third' band on the electrophoresis gel represented AE1, which was thus initially termed 'Band 3'. The chloride-bicarbonate exchanger in the red cell membrane is not a pump, which would use metabolic energy.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB681Hu01 Recombinant Band 3 (BND3) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB681Hu01 Polyclonal Antibody to Band 3 (BND3) WB; IHC; ICC; IP.
Assay Kits SEB681Hu ELISA Kit for Band 3 (BND3) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Band 3 (BND3) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Band 3 (BND3) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Band 3 (BND3) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Band 3 (BND3) CLIA Kit Customized Service Offer
n/a ELISA Kit for Band 3 (BND3) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB681Ra01 Recombinant Band 3 (BND3) Positive Control; Immunogen; SDS-PAGE; WB.
RPB681Ra02 Recombinant Band 3 (BND3) Positive Control; Immunogen; SDS-PAGE; WB.
APB681Ra01 Active Band 3 (BND3) Cell culture; Activity Assays.
Antibodies PAB681Ra01 Polyclonal Antibody to Band 3 (BND3) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Band 3 (BND3) CLIA Kit Customized Service Offer
n/a ELISA Kit for Band 3 (BND3) ELISA Kit Customized Service Offer
  1. "The complete amino acid sequence of the human erythrocyte membrane anion-transport protein deduced from the cDNA sequence."Biochem. J. 256:703-712(1988) [PubMed] [Europe PMC] [Abstract]
  2. "Cloning and characterization of band 3, the human erythrocyte anion-exchange protein (AE1)."Proc. Natl. Acad. Sci. U.S.A. 86:9089-9093(1989) [PubMed] [Europe PMC] [Abstract]
  3. "Recessive distal renal tubular acidosis in Sarawak caused by AE1 mutations."Pediatr. Nephrol. 21:212-217(2006) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Primary structure of the cytoplasmic domain of human erythrocyte protein band 3. Comparison with its sequence in the mouse."Biochim. Biophys. Acta 998:43-49(1989) [PubMed] [Europe PMC] [Abstract]
  6. "Amino acid sequence of the N alpha-terminal 201 residues of human erythrocyte membrane band 3."J. Biol. Chem. 258:7981-7990(1983) [PubMed] [Europe PMC] [Abstract]
  7. "Orientation of the band 3 polypeptide from human erythrocyte membranes. Identification of NH2-terminal sequence and site of carbohydrate attachment."J. Biol. Chem. 253:7242-7248(1978) [PubMed] [Europe PMC] [Abstract]
  8. "Anion exchanger 1 in human kidney and oncocytoma differs from erythroid AE1 in its NH2 terminus."Am. J. Physiol. 265:F813-F821(1993) [PubMed] [Europe PMC] [Abstract]
  9. "A structural study of the membrane domain of band 3 by tryptic digestion. Conformational change of band 3 in situ induced by alkali treatment."J. Biol. Chem. 267:19211-19217(1992) [PubMed] [Europe PMC] [Abstract]
  10. "The human erythrocyte anion-transport protein. Partial amino acid sequence, conformation and a possible molecular mechanism for anion exchange."Biochem. J. 213:577-586(1983) [PubMed] [Europe PMC] [Abstract]
  11. "Anion-proton cotransport through the human red blood cell band 3 protein. Role of glutamate 681."J. Biol. Chem. 267:13964-13971(1992) [PubMed] [Europe PMC] [Abstract]
  12. "Band 3 Chur: a variant associated with band 3-deficient hereditary spherocytosis and substitution in a highly conserved position of transmembrane segment 11."Br. J. Haematol. 91:804-810(1995) [PubMed] [Europe PMC] [Abstract]
  13. "Localization of the pyridoxal phosphate binding site at the COOH-terminal region of erythrocyte band 3 protein."J. Biol. Chem. 263:8232-8238(1988) [PubMed] [Europe PMC] [Abstract]
  14. "Phosphorylation sites in human erythrocyte band 3 protein."Biochim. Biophys. Acta 1061:253-266(1991) [PubMed] [Europe PMC] [Abstract]
  15. "Palmitoylation of cysteine 69 from the COOH-terminal of band 3 protein in the human erythrocyte membrane. Acylation occurs in the middle of the consensus sequence of F--I-IICLAVL found in band 3 protein and G2 protein of Rift Valley fever virus."J. Biol. Chem. 266:16420-16424(1991) [PubMed] [Europe PMC] [Abstract]
  16. "The ANK repeats of erythrocyte ankyrin form two distinct but cooperative binding sites for the erythrocyte anion exchanger."J. Biol. Chem. 270:22050-22057(1995) [PubMed] [Europe PMC] [Abstract]
  17. "Processing of N-linked oligosaccharide depends on its location in the anion exchanger, AE1, membrane glycoprotein."Biochem. J. 349:51-57(2000) [PubMed] [Europe PMC] [Abstract]
  18. "Sequential phosphorylation of protein band 3 by Syk and Lyn tyrosine kinases in intact human erythrocytes: identification of primary and secondary phosphorylation sites."Blood 96:1550-1557(2000) [PubMed] [Europe PMC] [Abstract]
  19. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  20. "Stomatin interacts with GLUT1/SLC2A1, band 3/SLC4A1, and aquaporin-1 in human erythrocyte membrane domains."Biochim. Biophys. Acta 1828:956-966(2013) [PubMed] [Europe PMC] [Abstract]
  21. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  22. "Two-dimensional structure of the membrane domain of human band 3, the anion transport protein of the erythrocyte membrane."EMBO J. 12:2233-2239(1993) [PubMed] [Europe PMC] [Abstract]
  23. "Three-dimensional map of the dimeric membrane domain of the human erythrocyte anion exchanger, Band 3."EMBO J. 13:3230-3235(1994) [PubMed] [Europe PMC] [Abstract]
  24. "The solution structures of the first and second transmembrane-spanning segments of band 3."Eur. J. Biochem. 221:445-454(1994) [PubMed] [Europe PMC] [Abstract]
  25. "Solution structure of a band 3 peptide inhibitor bound to aldolase: a proposed mechanism for regulating binding by tyrosine phosphorylation."Biochemistry 34:16574-16584(1995) [PubMed] [Europe PMC] [Abstract]
  26. "Insights into tyrosine phosphorylation control of protein-protein association from the NMR structure of a band 3 peptide inhibitor bound to glyceraldehyde-3-phosphate dehydrogenase."Biochemistry 37:867-877(1998) [PubMed] [Europe PMC] [Abstract]
  27. "Studies on the structure of a transmembrane region and a cytoplasmic loop of the human red cell anion exchanger."Biochem. Soc. Trans. 26:516-520(1998) [PubMed] [Europe PMC] [Abstract]
  28. "NMR solution structure of a cytoplasmic surface loop of the human red cell anion transporter, band 3."Biochemistry 37:11670-11678(1998) [PubMed] [Europe PMC] [Abstract]
  29. "Crystallographic structure and functional interpretation of the cytoplasmic domain of erythrocyte membrane band 3."Blood 96:2925-2933(2000) [PubMed] [Europe PMC] [Abstract]
  30. "A substrate access tunnel in the cytosolic domain is not an essential feature of the solute carrier 4 (SLC4) family of bicarbonate transporters."J. Biol. Chem. 288:33848-33860(2013) [PubMed] [Europe PMC] [Abstract]
  31. "Human erythrocyte band 3 polymorphism (band 3 Memphis): characterization of the structural modification (Lys 56-->Glu) by protein chemistry methods."Blood 78:1117-1120(1991) [PubMed] [Europe PMC] [Abstract]
  32. "Deletion in erythrocyte band 3 gene in malaria-resistant Southeast Asian ovalocytosis."Proc. Natl. Acad. Sci. U.S.A. 88:11022-11026(1991) [PubMed] [Europe PMC] [Abstract]
  33. "Band 3 Tuscaloosa: Pro-327-->Arg substitution in the cytoplasmic domain of erythrocyte band 3 protein associated with spherocytic hemolytic anemia and partial deficiency of protein 4.2."Blood 80:523-529(1992) [PubMed] [Europe PMC] [Abstract]
  34. "Basis of unique red cell membrane properties in hereditary ovalocytosis."J. Mol. Biol. 223:949-958(1992) [PubMed] [Europe PMC] [Abstract]
  35. "Band 3 HT, a human red-cell variant associated with acanthocytosis and increased anion transport, carries the mutation Pro-868-->Leu in the membrane domain of band 3."Biochem. J. 293:317-320(1993) [PubMed] [Europe PMC] [Abstract]
  36. "Human erythrocyte protein 4.2 deficiency associated with hemolytic anemia and a homozygous 40 glutamic acid-->lysine substitution in the cytoplasmic domain of band 3 (band 3Montefiore)."Blood 81:2155-2165(1993) [PubMed] [Europe PMC] [Abstract]
  37. "Band 3 Memphis variant II. Altered stilbene disulfonate binding and the Diego (Dia) blood group antigen are associated with the human erythrocyte band 3 mutation Pro-854-->Leu."J. Biol. Chem. 269:16155-16158(1994) [PubMed] [Europe PMC] [Abstract]
  38. "Changes in the blood group Wright antigens are associated with a mutation at amino acid 658 in human erythrocyte band 3: a site of interaction between band 3 and glycophorin A under certain conditions."Blood 85:541-547(1995) [PubMed] [Europe PMC] [Abstract]
  39. "Mutations of conserved arginines in the membrane domain of erythroid band 3 lead to a decrease in membrane-associated band 3 and to the phenotype of hereditary spherocytosis."Blood 85:634-640(1995) [PubMed] [Europe PMC] [Abstract]
  40. "Characterization of 13 novel band 3 gene defects in hereditary spherocytosis with band 3 deficiency."Blood 88:4366-4374(1996) [PubMed] [Europe PMC] [Abstract]
  41. "Ankyrin-1 mutations are a major cause of dominant and recessive hereditary spherocytosis."Nat. Genet. 13:214-218(1996) [PubMed] [Europe PMC] [Abstract]
  42. "Modulation of clinical expression and band 3 deficiency in hereditary spherocytosis."Blood 90:414-420(1997) [PubMed] [Europe PMC] [Abstract]
  43. "Novel band 3 variants (bands 3 Foggia, Napoli I and Napoli II) associated with hereditary spherocytosis and band 3 deficiency: status of the D38A polymorphism within the EPB3 locus."Br. J. Haematol. 96:70-76(1997) [PubMed] [Europe PMC] [Abstract]
  44. "Heterogenous band 3 deficiency in hereditary spherocytosis related to different band 3 gene defects."Br. J. Haematol. 98:32-40(1997) [PubMed] [Europe PMC] [Abstract]
  45. ErratumBr. J. Haematol. 99:474-474(1997)
  46. "Familial distal renal tubular acidosis is associated with mutations in the red cell anion exchanger (Band 3, AE1) gene."J. Clin. Invest. 100:1693-1707(1997) [PubMed] [Europe PMC] [Abstract]
  47. "Blood group antigens Rb(a), Tr(a), and Wd(a) are located in the third ectoplasmic loop of erythroid band 3."Transfusion 37:607-615(1997) [PubMed] [Europe PMC] [Abstract]
  48. "Band 3 Tokyo: Thr837-->Ala837 substitution in erythrocyte band 3 protein associated with spherocytic hemolysis."Acta Haematol. 100:200-203(1998) [PubMed] [Europe PMC] [Abstract]
  49. "Characterization of seven low incidence blood group antigens carried by erythrocyte band 3 protein."Blood 92:4836-4843(1998) [PubMed] [Europe PMC] [Abstract]
  50. "Novel AE1 mutations in recessive distal renal tubular acidosis: loss-of-function is rescued by glycophorin A."J. Clin. Invest. 102:2173-2179(1998) [PubMed] [Europe PMC] [Abstract]
  51. "Mutations in the chloride-bicarbonate exchanger gene AE1 cause autosomal dominant but not autosomal recessive distal renal tubular acidosis."Proc. Natl. Acad. Sci. U.S.A. 95:6337-6342(1998) [PubMed] [Europe PMC] [Abstract]
  52. "A Gly565-->Ala substitution in human erythroid band 3 accounts for the Wu blood group polymorphism."Transfusion 38:745-748(1998) [PubMed] [Europe PMC] [Abstract]
  53. "Arginine 490 is a hot spot for mutation in the band 3 gene in hereditary spherocytosis."Eur. J. Haematol. 63:360-361(1999) [PubMed] [Europe PMC] [Abstract]
  54. "Band 3 mutations, renal tubular acidosis and South-East Asian ovalocytosis in Malaysia and Papua New Guinea: loss of up to 95% band 3 transport in red cells."Biochem. J. 350:41-51(2000) [PubMed] [Europe PMC] [Abstract]
  55. "Severe hereditary spherocytosis and distal renal tubular acidosis associated with the total absence of band 3."Blood 96:1602-1604(2000) [PubMed] [Europe PMC] [Abstract]
  56. "Characteristic features of the genotype and phenotype of hereditary spherocytosis in the Japanese population."Int. J. Hematol. 71:118-135(2000) [PubMed] [Europe PMC] [Abstract]
  57. "Trafficking and folding defects in hereditary spherocytosis mutants of the human red cell anion exchanger."Traffic 1:987-998(2000) [PubMed] [Europe PMC] [Abstract]
  58. "Amino acid substitutions in human erythroid protein band 3 account for the low-incidence antigens NFLD and BOW."Transfusion 40:325-329(2000) [PubMed] [Europe PMC] [Abstract]
  59. "An amino acid substitution in the putative second extracellular loop of RBC band 3 accounts for the Froese blood group polymorphism."Transfusion 40:1246-1249(2000) [PubMed] [Europe PMC] [Abstract]
  60. "Distinctive Swann blood group genotypes: molecular investigations."Vox Sang. 79:215-218(2000) [PubMed] [Europe PMC] [Abstract]
  61. "Band 3 Cape Town (E90K) causes severe hereditary spherocytosis in combination with band 3 Prague III."Br. J. Haematol. 113:689-693(2001) [PubMed] [Europe PMC] [Abstract]
  62. "Novel compound heterozygous SLC4A1 mutations in Thai patients with autosomal recessive distal renal tubular acidosis."Am. J. Kidney Dis. 44:64-70(2004) [PubMed] [Europe PMC] [Abstract]
  63. "A novel missense mutation in AE1 causing autosomal dominant distal renal tubular acidosis retains normal transport function but is mistargeted in polarized epithelial cells."J. Biol. Chem. 279:13833-13838(2004) [PubMed] [Europe PMC] [Abstract]
  64. "Band 3Tambau: a de novo mutation in the AE1 gene associated with hereditary spherocytosis. Implications for anion exchange and insertion into the red blood cell membrane."Eur. J. Haematol. 74:396-401(2005) [PubMed] [Europe PMC] [Abstract]
  65. "Monovalent cation leaks in human red cells caused by single amino-acid substitutions in the transport domain of the band 3 chloride-bicarbonate exchanger, AE1."Nat. Genet. 37:1258-1263(2005) [PubMed] [Europe PMC] [Abstract]
  66. "Impaired trafficking and intracellular retention of mutant kidney anion exchanger 1 proteins (G701D and A858D) associated with distal renal tubular acidosis."Mol. Membr. Biol. 27:92-103(2010) [PubMed] [Europe PMC] [Abstract]