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S100 Calcium Binding Protein A8 (S100A8)

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MRP8; MIF; 60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag; NIF; P8; Calgranulin A; Cystic fibrosis antigen; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8

S100 Calcium Binding Protein A8 (S100A8)
CCC B792
HGNC 10498
MGI 88244
PubMed 16931866
RGD 620265
UniProt P05109
Wiki S100A8

S100A8, a protein associated with a wide variety of inflammatory conditions, is heavily over-expressed in association with inflammation. Human S100A8 possesses neutrophil-repelling properties that result in an anti-inflammatory effect in vivo. The chemotactic activity of S100A8 on neutrophils was tested in Transwell chemotaxis assays. Analysis of the data indicates that S100A8 causes a repulsion of peripheral neutrophils, an activity that S100A8 loses upon its oxidation. S100A8 causes a strong anti-inflammatory effect in the rat air-pouch model of inflammation in vivo. These data highlight a naturally occurring novel anti-inflammatory pathway and provide potential molecular targets for the development of novel anti-inflammatory therapeutics.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB792Hu01 Recombinant S100 Calcium Binding Protein A8 (S100A8) Positive Control; Immunogen; SDS-PAGE; WB.
RPB792Hu02 Recombinant S100 Calcium Binding Protein A8 (S100A8) Positive Control; Immunogen; SDS-PAGE; WB.
APB792Hu01 Active S100 Calcium Binding Protein A8 (S100A8) Cell culture; Activity Assays.
Antibodies PAB792Hu01 Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
LAB792Hu71 Biotin-Linked Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC.
LAB792Hu81 FITC-Linked Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IF.
MAB792Hu21 Monoclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC.
PAB792Hu02 Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP; FCM
MAB792Hu24 Monoclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; ICC/IF
MAB792Hu22 Monoclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
MAB792Hu23 Monoclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
MAB792Hu25 Monoclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
MAB792Hu26 Monoclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
MAB792Hu28 Monoclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
MAB792Hu27 Monoclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
MAB792Hu29 Monoclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
Assay Kits SEB792Hu ELISA Kit for S100 Calcium Binding Protein A8 (S100A8) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB792Mu01 Recombinant S100 Calcium Binding Protein A8 (S100A8) Positive Control; Immunogen; SDS-PAGE; WB.
RPB792Mu02 Recombinant S100 Calcium Binding Protein A8 (S100A8) Positive Control; Immunogen; SDS-PAGE; WB.
EPB792Mu61 Eukaryotic S100 Calcium Binding Protein A8 (S100A8) Positive Control; Immunogen; SDS-PAGE; WB.
APB792Mu61 Active S100 Calcium Binding Protein A8 (S100A8) Cell culture; Activity Assays.
Antibodies PAB792Mu01 Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
LAB792Mu71 Biotin-Linked Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC.
LAB792Mu81 FITC-Linked Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IF.
PAB792Mu02 Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
MAB792Mu21 Monoclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
Assay Kits SEB792Mu ELISA Kit for S100 Calcium Binding Protein A8 (S100A8) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB792Ra01 Recombinant S100 Calcium Binding Protein A8 (S100A8) Positive Control; Immunogen; SDS-PAGE; WB.
APB792Ra01 Active S100 Calcium Binding Protein A8 (S100A8) Cell culture; Activity Assays.
Antibodies PAB792Ra01 Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
LAB792Ra71 Biotin-Linked Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC.
LAB792Ra81 FITC-Linked Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IF.
MAB792Ra21 Monoclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
Assay Kits SEB792Ra ELISA Kit for S100 Calcium Binding Protein A8 (S100A8) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Oryctolagus cuniculus (Rabbit)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB792Rb01 Recombinant S100 Calcium Binding Protein A8 (S100A8) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB792Rb51 Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
PAB792Rb01 Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for S100 Calcium Binding Protein A8 (S100A8) CLIA Kit Customized Service Offer
n/a ELISA Kit for S100 Calcium Binding Protein A8 (S100A8) ELISA Kit Customized Service Offer

Organism species: Canis familiaris; Canine (Dog)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB792Ca01 Recombinant S100 Calcium Binding Protein A8 (S100A8) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB792Ca01 Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for S100 Calcium Binding Protein A8 (S100A8) CLIA Kit Customized Service Offer
n/a ELISA Kit for S100 Calcium Binding Protein A8 (S100A8) ELISA Kit Customized Service Offer

Organism species: Sus scrofa; Porcine (Pig)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB792Po01 Recombinant S100 Calcium Binding Protein A8 (S100A8) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB792Po01 Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
Assay Kits SEB792Po ELISA Kit for S100 Calcium Binding Protein A8 (S100A8) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Bos taurus; Bovine (Cattle)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB792Bo01 Recombinant S100 Calcium Binding Protein A8 (S100A8) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB792Bo01 Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
LAB792Bo71 Biotin-Linked Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC.
LAB792Bo81 FITC-Linked Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IF.
MAB792Bo21 Monoclonal Antibody to S100 Calcium Binding Protein A8 (S100A8) WB; IHC; ICC; IP.
Assay Kits SEB792Bo ELISA Kit for S100 Calcium Binding Protein A8 (S100A8) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "A clue to the basic defect in cystic fibrosis from cloning the CF antigen gene."Nature 326:614-617(1987) [PubMed] [Europe PMC] [Abstract]
  2. "Two calcium-binding proteins in infiltrate macrophages of rheumatoid arthritis."Nature 330:80-82(1987) [PubMed] [Europe PMC] [Abstract]
  3. "Cloning and expression of two human genes encoding calcium-binding proteins that are regulated during myeloid differentiation."Mol. Cell. Biol. 8:2402-2410(1988) [PubMed] [Europe PMC] [Abstract]
  4. "The calcium-binding protein MRP-8 is produced by human pulmonary tumor cells."Biol. Chem. Hoppe-Seyler 372:1-4(1991) [PubMed] [Europe PMC] [Abstract]
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  6. "The DNA sequence and biological annotation of human chromosome 1." Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  8. "Host-parasite interaction in human onchocerciasis: identification and sequence analysis of a novel human calgranulin."Biochem. Biophys. Res. Commun. 221:454-458(1996) [PubMed] [Europe PMC] [Abstract]
  9. "In vitro antimicrobial activity of the human neutrophil cytosolic S-100 protein complex, calprotectin, against Capnocytophaga sputigena."J. Dent. Res. 72:517-523(1993) [PubMed] [Europe PMC] [Abstract]
  10. "Translocation of a small cytosolic calcium-binding protein (MRP-8) to plasma membrane correlates with human neutrophil activation."J. Biol. Chem. 267:19379-19382(1992) [PubMed] [Europe PMC] [Abstract]
  11. "Calprotectin-like protein is related to soluble organic matrix in calcium oxalate urinary stone."Biochem. Mol. Biol. Int. 34:309-313(1994) [PubMed] [Europe PMC] [Abstract]
  12. "Isolation of an ascitic oncodevelopmental protein exhibiting high sequence homology with calcium-binding protein MRP8."Biol. Chem. Hoppe-Seyler 375:789-792(1994) [PubMed] [Europe PMC] [Abstract]
  13. "Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes."Electrophoresis 13:960-969(1992) [PubMed] [Europe PMC] [Abstract]
  14. "Myeloid-related protein (MRP) 8 and MRP14, calcium-binding proteins of the S100 family, are secreted by activated monocytes via a novel, tubulin-dependent pathway."J. Biol. Chem. 272:9496-9502(1997) [PubMed] [Europe PMC] [Abstract]
  15. "The microbial receptor CEACAM3 is linked to the calprotectin complex in granulocytes."Biochem. Biophys. Res. Commun. 289:191-197(2001) [PubMed] [Europe PMC] [Abstract]
  16. "Proinflammatory activities of S100: proteins S100A8, S100A9, and S100A8/A9 induce neutrophil chemotaxis and adhesion."J. Immunol. 170:3233-3242(2003) [PubMed] [Europe PMC] [Abstract]
  17. "MRP8 and MRP14 control microtubule reorganization during transendothelial migration of phagocytes."Blood 104:4260-4268(2004) [PubMed] [Europe PMC] [Abstract]
  18. "Myeloid-related proteins 8 and 14 induce a specific inflammatory response in human microvascular endothelial cells."Blood 105:2955-2962(2005) [PubMed] [Europe PMC] [Abstract]
  19. "The arachidonic acid-binding protein S100A8/A9 promotes NADPH oxidase activation by interaction with p67phox and Rac-2."FASEB J. 19:467-469(2005) [PubMed] [Europe PMC] [Abstract]
  20. "Regulation of S100A8/A9 (calprotectin) binding to tumor cells by zinc ion and its implication for apoptosis-inducing activity."Mediators Inflamm. 2005:280-292(2005) [PubMed] [Europe PMC] [Abstract]
  21. "Interaction between S100A8/A9 and annexin A6 is involved in the calcium-induced cell surface exposition of S100A8/A9."J. Biol. Chem. 283:31776-31784(2008) [PubMed] [Europe PMC] [Abstract]
  22. "S-nitrosylated S100A8: novel anti-inflammatory properties."J. Immunol. 181:5627-5636(2008) [PubMed] [Europe PMC] [Abstract]
  23. "Anti-infective protective properties of S100 calgranulins."Antiinflamm. Antiallergy Agents Med. Chem. 8:290-305(2009) [PubMed] [Europe PMC] [Abstract]
  24. "S100A8/A9: a Janus-faced molecule in cancer therapy and tumorgenesis."Eur. J. Pharmacol. 625:73-83(2009) [PubMed] [Europe PMC] [Abstract]
  25. "Substitution of methionine 63 or 83 in S100A9 and cysteine 42 in S100A8 abrogate the antifungal activities of S100A8/A9: potential role for oxidative regulation."FEMS Immunol. Med. Microbiol. 55:55-61(2009) [PubMed] [Europe PMC] [Abstract]
  26. "Calprotectin S100A9 calcium-binding loops I and II are essential for keratinocyte resistance to bacterial invasion."J. Biol. Chem. 284:7078-7090(2009) [PubMed] [Europe PMC] [Abstract]
  27. "The endogenous Toll-like receptor 4 agonist S100A8/S100A9 (calprotectin) as innate amplifier of infection, autoimmunity, and cancer."J. Leukoc. Biol. 86:557-566(2009) [PubMed] [Europe PMC] [Abstract]
  28. "S100A8/A9 induces autophagy and apoptosis via ROS-mediated cross-talk between mitochondria and lysosomes that involves BNIP3."Cell Res. 20:314-331(2010) [PubMed] [Europe PMC] [Abstract]
  29. "S100 Calgranulins in inflammatory arthritis."Immunol. Cell Biol. 88:41-49(2010) [PubMed] [Europe PMC] [Abstract]
  30. "Inflammation-associated S100 proteins: new mechanisms that regulate function."Amino Acids 41:821-842(2011) [PubMed] [Europe PMC] [Abstract]
  31. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  32. "S100A8 and S100A9 in cardiovascular biology and disease."Arterioscler. Thromb. Vasc. Biol. 32:223-229(2012) [PubMed] [Europe PMC] [Abstract]
  33. "Dynamic mobility of immunological cells expressing S100A8 and S100A9 in vivo: a variety of functional roles of the two proteins as regulators in acute inflammatory reaction."Inflammation 35:409-419(2012) [PubMed] [Europe PMC] [Abstract]
  34. "Pro-inflammatory S100A8 and S100A9 proteins: self-assembly into multifunctional native and amyloid complexes."Int. J. Mol. Sci. 13:2893-2917(2012) [PubMed] [Europe PMC] [Abstract]
  35. "S100A8 and S100A9: new insights into their roles in malignancy."J. Innate Immun. 4:31-40(2012) [PubMed] [Europe PMC] [Abstract]
  36. "Molecular interface of S100A8 with cytochrome b and NADPH oxidase activation."PLoS ONE 7:E40277-E40277(2012) [PubMed] [Europe PMC] [Abstract]
  37. "Constitutive neutrophil apoptosis: regulation by cell concentration via S100 A8/9 and the MEK-ERK pathway."PLoS ONE 7:E29333-E29333(2012) [PubMed] [Europe PMC] [Abstract]
  38. "Target-selective protein S-nitrosylation by sequence motif recognition."Cell 159:623-634(2014) [PubMed] [Europe PMC] [Abstract]
  39. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  40. "The structure of human MRP8, a member of the S100 calcium-binding protein family, by MAD phasing at 1.9 A resolution."Acta Crystallogr. D 56:559-566(2000) [PubMed] [Europe PMC] [Abstract]
  41. "The crystal structure of the human (S100A8/S100A9)2 heterotetramer, calprotectin, illustrates how conformational changes of interacting alpha-helices can determine specific association of two EF-hand proteins."J. Mol. Biol. 370:887-898(2007) [PubMed] [Europe PMC] [Abstract]