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Bruton'S Tyrosine Kinase (Btk)

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AGMX1; AT; ATK; BPK; IMD1; PSCTK1; XLA; Bruton Agammaglobulinemia Tyrosine Kinase; B-cell progenitor kinase

Bruton'S Tyrosine Kinase (Btk)
CCC B915
HGNC 1133
MGI 88216
PubMed 21422473
RGD 1359683
UniProt Q06187
Wiki BTK

Bruton's tyrosine kinase (or Btk) is a type of kinase enzyme implicated in the primary immunodeficiency disease X-linked agammaglobulinemia (Bruton's agammaglobulinemia). Patients with XLA have normal pre-B cell populations in their bone marrow but these cells fail to mature and enter the circulation. The BTK gene is located on the X chromosome. At least 400 mutations of the BTK gene have been identified. Btk contains a PH domain which binds Phosphatidylinositol (3,4,5)-trisphosphate (PIP3). PIP3 binding induces Btk to phosphorylate phospholipase C, which in turn hydrolyzes PIP2, a phosphatidylinositol, into two second messagers, inositol triphosphate (IP3) and diacylglycerol(DAG), which then go on to modulate the activity of downstream proteins during B-cell signalling.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB915Hu01 Recombinant Bruton'S Tyrosine Kinase (Btk) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB915Hu01 Polyclonal Antibody to Bruton'S Tyrosine Kinase (Btk) WB; IHC; ICC; IP.
MAB915Hu22 Monoclonal Antibody to Bruton'S Tyrosine Kinase (Btk) WB; IHC; ICC; IP.
Assay Kits SEB915Hu ELISA Kit for Bruton'S Tyrosine Kinase (Btk) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB915Mu01 Recombinant Bruton'S Tyrosine Kinase (Btk) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB915Mu01 Polyclonal Antibody to Bruton'S Tyrosine Kinase (Btk) WB; IHC; ICC; IP.
Assay Kits SEB915Mu ELISA Kit for Bruton'S Tyrosine Kinase (Btk) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB915Ra01 Recombinant Bruton'S Tyrosine Kinase (Btk) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB915Ra01 Polyclonal Antibody to Bruton'S Tyrosine Kinase (Btk) WB; IHC; ICC; IP.
MAB915Ra21 Monoclonal Antibody to Bruton'S Tyrosine Kinase (Btk) WB; IHC; ICC; IP.
Assay Kits SEB915Ra ELISA Kit for Bruton'S Tyrosine Kinase (Btk) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "The gene involved in X-linked agammaglobulinaemia is a member of the src family of protein-tyrosine kinases."Nature 361:226-233(1993) [PubMed] [Europe PMC] [Abstract]
  2. ErratumNature 364:362-362(1993)
  3. "Genomic organization and structure of Bruton agammaglobulinemia tyrosine kinase: localization of mutations associated with varied clinical presentations and course in X chromosome-linked agammaglobulinemia."Proc. Natl. Acad. Sci. U.S.A. 91:9062-9066(1994) [PubMed] [Europe PMC] [Abstract]
  4. "The genomic structure of human BTK, the defective gene in X-linked agammaglobulinemia."Immunogenetics 40:319-324(1994) [PubMed] [Europe PMC] [Abstract]
  5. "Genomic organization of the Btk gene and exon scanning for mutations in patients with X-linked agammaglobulinemia."Hum. Mol. Genet. 3:1743-1749(1994) [PubMed] [Europe PMC] [Abstract]
  6. "Sixty-nine kilobases of contiguous human genomic sequence containing the alpha-galactosidase A and Bruton's tyrosine kinase loci."Mamm. Genome 6:334-338(1995) [PubMed] [Europe PMC] [Abstract]
  7. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  8. "The DNA sequence of the human X chromosome." Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
  9. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  10. "Deficient expression of a B cell cytoplasmic tyrosine kinase in human X-linked agammaglobulinemia."Cell 72:279-290(1993) [PubMed] [Europe PMC] [Abstract]
  11. , Submitted (NOV-2005) to UniProtKB
  12. "Identification of phosphorylation sites within the SH3 domains of Tec family tyrosine kinases."Biochim. Biophys. Acta 1645:123-132(2003) [PubMed] [Europe PMC] [Abstract]
  13. "An exon-skipping mutation in the btk gene of a patient with X-linked agammaglobulinemia and isolated growth hormone deficiency."FEBS Lett. 346:165-170(1994) [PubMed] [Europe PMC] [Abstract]
  14. "Tec homology (TH) adjacent to the PH domain."FEBS Lett. 350:263-265(1994) [PubMed] [Europe PMC] [Abstract]
  15. "Regulation of Btk function by a major autophosphorylation site within the SH3 domain."Immunity 4:515-525(1996) [PubMed] [Europe PMC] [Abstract]
  16. "BAP-135, a target for Bruton's tyrosine kinase in response to B cell receptor engagement."Proc. Natl. Acad. Sci. U.S.A. 94:604-609(1997) [PubMed] [Europe PMC] [Abstract]
  17. "Identification and characterization of a novel SH3-domain binding protein, Sab, which preferentially associates with Bruton's tyrosine kinase (Btk)."Biochem. Biophys. Res. Commun. 245:337-343(1998) [PubMed] [Europe PMC] [Abstract]
  18. "Phosphatidylinositol 3-kinase-dependent membrane association of the Bruton's tyrosine kinase pleckstrin homology domain visualized in single living cells."J. Biol. Chem. 274:10983-10989(1999) [PubMed] [Europe PMC] [Abstract]
  19. "Bruton's tyrosine kinase activity is negatively regulated by Sab, the Btk-SH3 domain-binding protein."Proc. Natl. Acad. Sci. U.S.A. 96:6341-6346(1999) [PubMed] [Europe PMC] [Abstract]
  20. "Redistribution of Bruton's tyrosine kinase by activation of phosphatidylinositol 3-kinase and Rho-family GTPases."Eur. J. Immunol. 30:145-154(2000) [PubMed] [Europe PMC] [Abstract]
  21. "Nucleocytoplasmic shuttling of Bruton's tyrosine kinase."J. Biol. Chem. 275:40614-40619(2000) [PubMed] [Europe PMC] [Abstract]
  22. "PKCbeta modulates antigen receptor signaling via regulation of Btk membrane localization."EMBO J. 20:5692-5702(2001) [PubMed] [Europe PMC] [Abstract]
  23. "Tyrosine residues in phospholipase Cgamma 2 essential for the enzyme function in B-cell signaling."J. Biol. Chem. 276:47982-47992(2001) [PubMed] [Europe PMC] [Abstract]
  24. "Direct inhibition of Bruton's tyrosine kinase by IBtk, a Btk-binding protein."Nat. Immunol. 2:939-946(2001) [PubMed] [Europe PMC] [Abstract]
  25. "Functional interaction of caveolin-1 with Bruton's tyrosine kinase and Bmx."J. Biol. Chem. 277:9351-9357(2002) [PubMed] [Europe PMC] [Abstract]
  26. "A phosphorylation site in Bruton's tyrosine kinase selectively regulates B cell calcium signaling efficiency by altering phospholipase C-gamma activation."Proc. Natl. Acad. Sci. U.S.A. 101:14180-14185(2004) [PubMed] [Europe PMC] [Abstract]
  27. "Regulation of Bruton tyrosine kinase by the peptidylprolyl isomerase Pin1."J. Biol. Chem. 281:18201-18207(2006) [PubMed] [Europe PMC] [Abstract]
  28. "Bruton's tyrosine kinase is required for TLR2 and TLR4-induced TNF, but not IL-6, production."J. Immunol. 176:3635-3641(2006) [PubMed] [Europe PMC] [Abstract]
  29. "Induction of immunoglobulin heavy-chain transcription through the transcription factor Bright requires TFII-I."Mol. Cell. Biol. 26:4758-4768(2006) [PubMed] [Europe PMC] [Abstract]
  30. "Suppressor of cytokine signaling 1 negatively regulates Toll-like receptor signaling by mediating Mal degradation."Nat. Immunol. 7:148-155(2006) [PubMed] [Europe PMC] [Abstract]
  31. "Signaling by Toll-like receptors 8 and 9 requires Bruton's tyrosine kinase."J. Biol. Chem. 282:36953-36960(2007) [PubMed] [Europe PMC] [Abstract]
  32. "Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening."BMC Immunol. 10:53-53(2009) [PubMed] [Europe PMC] [Abstract]
  33. "Bruton's tyrosine kinase (BTK) as a dual-function regulator of apoptosis."Biochem. Pharmacol. 56:683-691(1998) [PubMed] [Europe PMC] [Abstract]
  34. "Bruton's tyrosine kinase (Btk): function, regulation, and transformation with special emphasis on the PH domain."Immunol. Rev. 228:58-73(2009) [PubMed] [Europe PMC] [Abstract]
  35. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  36. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  37. "A novel isoform of the B cell tyrosine kinase BTK protects breast cancer cells from apoptosis."Genes Chromosomes Cancer 52:961-975(2013) [PubMed] [Europe PMC] [Abstract]
  38. "Structure of the PH domain and Btk motif from Bruton's tyrosine kinase: molecular explanations for X-linked agammaglobulinaemia."EMBO J. 16:3396-3404(1997) [PubMed] [Europe PMC] [Abstract]
  39. "Solution structure of the SH3 domain from Bruton's tyrosine kinase."Biochemistry 37:2912-2924(1998) [PubMed] [Europe PMC] [Abstract]
  40. "Structure of the PH domain from Bruton's tyrosine kinase in complex with inositol 1,3,4,5-tetrakisphosphate."Structure 7:449-460(1999) [PubMed] [Europe PMC] [Abstract]
  41. "Solution structure of the human BTK SH3 domain complexed with a proline-rich peptide from p120cbl."J. Biomol. NMR 16:303-312(2000) [PubMed] [Europe PMC] [Abstract]
  42. "Crystal structure of Bruton's tyrosine kinase domain suggests a novel pathway for activation and provides insights into the molecular basis of X-linked agammaglobulinemia."J. Biol. Chem. 276:41435-41443(2001) [PubMed] [Europe PMC] [Abstract]
  43. "Solution structure and phosphopeptide binding of the SH2 domain from the human Bruton's tyrosine kinase."J. Biomol. NMR 36:73-78(2006) [PubMed] [Europe PMC] [Abstract]
  44. "A novel, specific Btk inhibitor antagonizes BCR and Fc[gamma]R signaling and suppresses inflammatory arthritis."Submitted (AUG-2010) to the PDB data bank
  45. "Crystal structure of PH domain of Bruton's tyrosine kinase."Submitted (MAY-2007) to the PDB data bank
  46. "Structures of human Bruton's tyrosine kinase in active and inactive conformations suggest a mechanism of activation for TEC family kinases."Protein Sci. 19:429-439(2010) [PubMed] [Europe PMC] [Abstract]
  47. "A novel, specific BTK inhibitor antagonizes BCR and FcgR signaling and suppresses inflammatory arthritis." Submitted (SEP-2010) to the PDB data bank
  48. "Insights into the conformational flexibility of Bruton's tyrosine kinase from multiple ligand complex structures."Protein Sci. 20:428-436(2011) [PubMed] [Europe PMC] [Abstract]
  49. "BTKbase, mutation database for X-linked agammaglobulinemia (XLA)."Nucleic Acids Res. 24:160-165(1996) [PubMed] [Europe PMC] [Abstract]
  50. "BTKbase, mutation database for X-linked agammaglobulinemia (XLA)."Nucleic Acids Res. 25:166-171(1997) [PubMed] [Europe PMC] [Abstract]
  51. "Mutation detection in the X-linked agammaglobulinemia gene, BTK, using single strand conformation polymorphism analysis."Hum. Mol. Genet. 3:79-83(1994) [PubMed] [Europe PMC] [Abstract]
  52. "Mutation analysis of the Bruton's tyrosine kinase gene in X-linked agammaglobulinemia: identification of a mutation which affects the same codon as is altered in immunodeficient xid mice."Hum. Mol. Genet. 3:161-166(1994) [PubMed] [Europe PMC] [Abstract]
  53. "Screening of genomic DNA to identify mutations in the gene for Bruton's tyrosine kinase."Hum. Mol. Genet. 3:1751-1756(1994) [PubMed] [Europe PMC] [Abstract]
  54. "Unique mutations of Bruton's tyrosine kinase in fourteen unrelated X-linked agammaglobulinemia families."Hum. Mol. Genet. 3:1899-1900(1994) [PubMed] [Europe PMC] [Abstract]
  55. "Structural basis for chromosome X-linked agammaglobulinemia: a tyrosine kinase disease."Proc. Natl. Acad. Sci. U.S.A. 91:12803-12807(1994) [PubMed] [Europe PMC] [Abstract]
  56. "Structural basis for pleckstrin homology domain mutations in X-linked agammaglobulinemia."Biochemistry 34:1475-1481(1995) [PubMed] [Europe PMC] [Abstract]
  57. "DNA-based mutation analysis of Bruton's tyrosine kinase gene in patients with X-linked agammaglobulinaemia."Hum. Mol. Genet. 4:51-58(1995) [PubMed] [Europe PMC] [Abstract]
  58. "Identification of Btk mutations in 20 unrelated patients with X-linked agammaglobulinaemia (XLA)."Hum. Mol. Genet. 4:693-700(1995) [PubMed] [Europe PMC] [Abstract]
  59. "Mutation analysis in Bruton's tyrosine kinase, the X-linked agammaglobulinaemia gene, including identification of an insertional hotspot."Hum. Mol. Genet. 4:755-757(1995) [PubMed] [Europe PMC] [Abstract]
  60. "Improved oligonucleotide primer set for molecular diagnosis of X-linked agammaglobulinaemia: predominance of amino acid substitutions in the catalytic domain of Bruton's tyrosine kinase."Hum. Mol. Genet. 4:2403-2405(1995) [PubMed] [Europe PMC] [Abstract]
  61. "Characterization of germline mutations of the gene encoding Bruton's tyrosine kinase in families with X-linked agammaglobulinemia."Hum. Mutat. 5:296-302(1995) [PubMed] [Europe PMC] [Abstract]
  62. "A new point mutation involving a highly conserved leucine in the Btk SH2 domain in a family with X linked agammaglobulinaemia."J. Med. Genet. 32:77-79(1995) [PubMed] [Europe PMC] [Abstract]
  63. "Detection of a novel mutation in the SRC homology domain 2 (SH2) of Bruton's tyrosine kinase and direct female carrier evaluation in a family with X-linked agammaglobulinemia."Am. J. Med. Genet. 63:318-322(1996) [PubMed] [Europe PMC] [Abstract]
  64. "Identification of Bruton's tyrosine kinase (Btk) gene mutations and characterization of the derived proteins in 35 X-linked agammaglobulinemia families: a nationwide study of Btk deficiency in Japan."Blood 88:561-573(1996) [PubMed] [Europe PMC] [Abstract]
  65. "Mutations of the Btk gene in 12 unrelated families with X-linked agammaglobulinemia in Japan."Hum. Genet. 97:424-430(1996) [PubMed] [Europe PMC] [Abstract]
  66. "Missense mutations affecting a conserved cysteine pair in the TH domain of Btk."FEBS Lett. 413:205-210(1997) [PubMed] [Europe PMC] [Abstract]
  67. "Molecular and structural characterization of five novel mutations in the Bruton's tyrosine kinase gene from patients with X-linked agammaglobulinemia."Mol. Med. 3:477-485(1997) [PubMed] [Europe PMC] [Abstract]
  68. "Mutations in btk in patients with presumed X-linked agammaglobulinemia."Am. J. Hum. Genet. 62:1034-1043(1998) [PubMed] [Europe PMC] [Abstract]
  69. "Mutation screening of the BTK gene in 56 families with X-linked agammaglobulinemia (XLA): 47 unique mutations without correlation to clinical course."Pediatrics 101:276-284(1998) [PubMed] [Europe PMC] [Abstract]
  70. "Mutations of the human BTK gene coding for Bruton tyrosine kinase in X-linked agammaglobulinemia."Hum. Mutat. 13:280-285(1999) [PubMed] [Europe PMC] [Abstract]
  71. "Twin carriers of X-linked agammaglobulinemia (XLA) due to germline mutation in the Btk gene."Am. J. Med. Genet. 90:229-232(2000) [PubMed] [Europe PMC] [Abstract]
  72. "Identification of nine novel mutations in the Bruton's tyrosine kinase gene in X-linked agammaglobulinaemia patients."Hum. Mutat. 15:117-117(2000) [PubMed] [Europe PMC] [Abstract]
  73. "Patterns of somatic mutation in human cancer genomes." Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]