≡

Arachidonate-12-Lipoxygenase (ALOX12)

[Edit]

LOX12; 12-LOX; ALOX-12; 12-LO; 12LPG; LOG12; Lipoxygenase 12; 12S-lipoxygenase; Lipoxin synthase 12-LO; Platelet-type lipoxygenase 12

Arachidonate-12-Lipoxygenase (ALOX12)
CCC B965
HGNC 429
MGI 87998
PubMed 19555433
RGD 1311159
UniProt P18054
Wiki ALOX12

Arachidonate 12-lipoxygenase introduces a molecular oxygen into the C-12 position of arachidonic acid to produce 12(S)-hydroperoxy-5,8,10,14-eicosatetraenoic acid. The major pathway of arachidonic acid metabolism in human platelets proceeds via a 12-lipoxygenase enzyme. Expression of the LOG12 gene was detected in human erythroleukemia cells, platelets, and human umbilical vein endothelial cells by reverse transcription-PCR analysis. The presumed pseudogene showed no detectable expression in several tissues and cell lines. Arachidonate 12-lipoxygenase introduces a molecular oxygen into C-12 of arachidonic acid. The enzyme is present also in leukocytes and has been found in various mammalian tissues such as porcine and bovine leukocytes, murine eosinophils, bovine tracheal cells, and porcine pituitary cells.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB965Hu01 Recombinant Arachidonate-12-Lipoxygenase (ALOX12) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB965Hu01 Polyclonal Antibody to Arachidonate-12-Lipoxygenase (ALOX12) WB; IHC; ICC; IP.
MAB965Hu21 Monoclonal Antibody to Arachidonate-12-Lipoxygenase (ALOX12) WB; IHC; ICC; IP.
Assay Kits SEB965Hu ELISA Kit for Arachidonate-12-Lipoxygenase (ALOX12) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Arachidonate-12-Lipoxygenase (ALOX12) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Arachidonate-12-Lipoxygenase (ALOX12) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Arachidonate-12-Lipoxygenase (ALOX12) Polyclonal Antibody Customized Service Offer
Assay Kits SEB965Mu ELISA Kit for Arachidonate-12-Lipoxygenase (ALOX12) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Arachidonate-12-Lipoxygenase (ALOX12) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Arachidonate-12-Lipoxygenase (ALOX12) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Arachidonate-12-Lipoxygenase (ALOX12) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Arachidonate-12-Lipoxygenase (ALOX12) CLIA Kit Customized Service Offer
n/a ELISA Kit for Arachidonate-12-Lipoxygenase (ALOX12) ELISA Kit Customized Service Offer
  1. "Molecular cloning and expression of human arachidonate 12-lipoxygenase."Biochem. Biophys. Res. Commun. 172:1230-1235(1990) [PubMed] [Europe PMC] [Abstract]
  2. "Molecular cloning, primary structure, and expression of the human platelet/erythroleukemia cell 12-lipoxygenase."Proc. Natl. Acad. Sci. U.S.A. 87:5638-5642(1990) [PubMed] [Europe PMC] [Abstract]
  3. "Cloning of the cDNA for human 12-lipoxygenase."Proc. Natl. Acad. Sci. U.S.A. 87:7477-7481(1990) [PubMed] [Europe PMC] [Abstract]
  4. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Structure and chromosomal localization of human arachidonate 12-lipoxygenase gene."J. Biol. Chem. 267:24805-24809(1992) [PubMed] [Europe PMC] [Abstract]
  7. "Characterization of human 12-lipoxygenase genes."Proc. Natl. Acad. Sci. U.S.A. 89:3962-3966(1992) [PubMed] [Europe PMC] [Abstract]
  8. "Epidermis contains platelet-type 12-lipoxygenase that is overexpressed in germinal layer keratinocytes in psoriasis."Am. J. Physiol. 266:C243-C253(1994) [PubMed] [Europe PMC] [Abstract]
  9. "Catalytic properties of human platelet 12-lipoxygenase as compared with the enzymes of other origins."Biochim. Biophys. Acta 1083:89-93(1991) [PubMed] [Europe PMC] [Abstract]
  10. "Lipoxin synthase activity of human platelet 12-lipoxygenase."Biochem. J. 296:127-133(1993) [PubMed] [Europe PMC] [Abstract]
  11. "Purification and characterization of recombinant histidine-tagged human platelet 12-lipoxygenase expressed in a baculovirus/insect cell system."Eur. J. Biochem. 214:845-852(1993) [PubMed] [Europe PMC] [Abstract]
  12. "Structure-function properties of human platelet 12-lipoxygenase: chimeric enzyme and in vitro mutagenesis studies."FASEB J. 7:694-701(1993) [PubMed] [Europe PMC] [Abstract]
  13. "12-Lipoxygenase in A431 cells: genetic identity, modulation of expression, and intracellular localization."Exp. Cell Res. 228:197-205(1996) [PubMed] [Europe PMC] [Abstract]
  14. "Platelet-type 12-lipoxygenase in a human prostate carcinoma stimulates angiogenesis and tumor growth."Cancer Res. 58:4047-4051(1998) [PubMed] [Europe PMC] [Abstract]
  15. "Mechanisms regulating tumor angiogenesis by 12-lipoxygenase in prostate cancer cells."J. Biol. Chem. 281:18601-18609(2006) [PubMed] [Europe PMC] [Abstract]
  16. "Reciprocal regulation of 12- and 15-lipoxygenases by UV-irradiation in human keratinocytes."FEBS Lett. 582:3249-3253(2008) [PubMed] [Europe PMC] [Abstract]
  17. "Up-regulation of 12(S)-lipoxygenase induces a migratory phenotype in colorectal cancer cells."Exp. Cell Res. 318:768-778(2012) [PubMed] [Europe PMC] [Abstract]
  18. "12S-Lipoxygenase is necessary for human vascular smooth muscle cell survival."Exp. Cell Res. 319:1586-1593(2013) [PubMed] [Europe PMC] [Abstract]
  19. "Crystal structure of the lipoxygenase domain of human arachidonate 12-lipoxygenase, 12s-type."Submitted (FEB-2009) to the PDB data bank
  20. "Arachidonate lipoxygenase (ALOX) and cyclooxygenase (COX) polymorphisms and colon cancer risk."Carcinogenesis 25:2467-2472(2004) [PubMed] [Europe PMC] [Abstract]
  21. "Associations of functional polymorphisms in cyclooxygenase-2 and platelet 12-lipoxygenase with risk of occurrence and advanced disease status of colorectal cancer."Carcinogenesis 28:1197-1201(2007) [PubMed] [Europe PMC] [Abstract]
  22. "Platelet 12-lipoxygenase Arg261Gln polymorphism: functional characterization and association with risk of esophageal squamous cell carcinoma in combination with COX-2 polymorphisms."Pharmacogenet. Genomics 17:197-205(2007) [PubMed] [Europe PMC] [Abstract]