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Protein Kinase N2 (PKN2)

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PRK2; Pak-2; PRKCL2; Protein Kinase C-Like 2; Protein-kinase C-related kinase 2; Serine/threonine-protein kinase N2; Cardiolipin-Activated Protein Kinase Pak2

Protein Kinase N2 (PKN2)
CCC B987
HGNC 9406
MGI 109211
PubMed 8932326
RGD 620146
UniProt Q16513
Wiki PKN2

The cDNA sequence predicts a 984-amino acid polypeptide. Expression of this cDNA in COS cells produced a 130-kD protein product that was capable of autophosphorylation, indicating intrinsic kinase activity. Using Northern blot and RT-PCR analyses to examine expression patterns in a wide variety of tissues, Palmer et al. (1995) detected PRK2 expression in DX3 B-cell myeloma cells only. Yu et al. (1997) purified a protein kinase, termed PAK2, from rat liver and showed that the corresponding gene is the rat homolog of human PRK2. PAK2 has biochemical properties similar to, but distinct from PRK1/PAK1. PAK2 is activated by lipids, particularly cardiolipin.The International Radiation Hybrid Mapping Consortium mapped the PKN2 gene to chromosome 1

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB987Hu01 Recombinant Protein Kinase N2 (PKN2) Positive Control; Immunogen; SDS-PAGE; WB.
RPB987Hu02 Recombinant Protein Kinase N2 (PKN2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB987Hu01 Polyclonal Antibody to Protein Kinase N2 (PKN2) WB; IHC; ICC; IP.
PAB987Hu02 Polyclonal Antibody to Protein Kinase N2 (PKN2) WB; IHC; ICC; IP.
LAB987Hu71 Biotin-Linked Polyclonal Antibody to Protein Kinase N2 (PKN2) WB; IHC; ICC.
Assay Kits n/a CLIA Kit for Protein Kinase N2 (PKN2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein Kinase N2 (PKN2) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB987Mu01 Recombinant Protein Kinase N2 (PKN2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB987Mu01 Polyclonal Antibody to Protein Kinase N2 (PKN2) WB; IHC; ICC; IP.
LAB987Mu71 Biotin-Linked Polyclonal Antibody to Protein Kinase N2 (PKN2) WB; IHC; ICC.
Assay Kits n/a CLIA Kit for Protein Kinase N2 (PKN2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein Kinase N2 (PKN2) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Protein Kinase N2 (PKN2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Protein Kinase N2 (PKN2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Protein Kinase N2 (PKN2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Protein Kinase N2 (PKN2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein Kinase N2 (PKN2) ELISA Kit Customized Service Offer
  1. "Identification of multiple, novel, protein kinase C-related gene products."FEBS Lett. 356:5-8(1994) [PubMed] [Europe PMC] [Abstract]
  2. "Cloning and expression patterns of two members of a novel protein-kinase-C-related kinase family."Eur. J. Biochem. 227:344-351(1995) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The DNA sequence and biological annotation of human chromosome 1." Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Specific proteolysis of the kinase protein kinase C-related kinase 2 by caspase-3 during apoptosis. Identification by a novel, small pool expression cloning strategy."J. Biol. Chem. 272:29449-29453(1997) [PubMed] [Europe PMC] [Abstract]
  7. "The PRK2 kinase is a potential effector target of both Rho and Rac GTPases and regulates actin cytoskeletal organization."Mol. Cell. Biol. 17:2247-2256(1997) [PubMed] [Europe PMC] [Abstract]
  8. "PDK1 acquires PDK2 activity in the presence of a synthetic peptide derived from the carboxyl terminus of PRK2."Curr. Biol. 9:393-404(1999) [PubMed] [Europe PMC] [Abstract]
  9. "Identification of Grb4/Nckbeta, a src homology 2 and 3 domain-containing adapter protein having similar binding and biological properties to Nck."J. Biol. Chem. 274:5542-5549(1999) [PubMed] [Europe PMC] [Abstract]
  10. "MEK kinase 2 binds and activates protein kinase C-related kinase 2. Bifurcation of kinase regulatory pathways at the level of an MAPK kinase kinase."J. Biol. Chem. 275:24421-24428(2000) [PubMed] [Europe PMC] [Abstract]
  11. "Inhibition of Akt and its anti-apoptotic activities by tumor necrosis factor-induced protein kinase C-related kinase 2 (PRK2) cleavage."J. Biol. Chem. 275:34451-34458(2000) [PubMed] [Europe PMC] [Abstract]
  12. "Phosphorylation of protein kinase N by phosphoinositide-dependent protein kinase-1 mediates insulin signals to the actin cytoskeleton."Proc. Natl. Acad. Sci. U.S.A. 97:5089-5094(2000) [PubMed] [Europe PMC] [Abstract]
  13. "The protein kinase C-related kinase PRK2 interacts with the protein tyrosine phosphatase PTP-BL via a novel PDZ domain binding motif."FEBS Lett. 496:101-104(2001) [PubMed] [Europe PMC] [Abstract]
  14. "Regulation of both PDK1 and the phosphorylation of PKC-zeta and -delta by a C-terminal PRK2 fragment."Biochemistry 41:561-569(2002) [PubMed] [Europe PMC] [Abstract]
  15. "Fyn tyrosine kinase is a downstream mediator of Rho/PRK2 function in keratinocyte cell-cell adhesion."J. Cell Biol. 156:137-148(2002) [PubMed] [Europe PMC] [Abstract]
  16. "Protein kinase C-related kinase 2 regulates hepatitis C virus RNA polymerase function by phosphorylation."J. Biol. Chem. 279:50031-50041(2004) [PubMed] [Europe PMC] [Abstract]
  17. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  18. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  19. "Rho GTPases regulate PRK2/PKN2 to control entry into mitosis and exit from cytokinesis."EMBO J. 26:1624-1636(2007) [PubMed] [Europe PMC] [Abstract]
  20. "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract]
  21. "The C-terminus of PRK2/PKNgamma is required for optimal activation by RhoA in a GTP-dependent manner."Arch. Biochem. Biophys. 479:170-178(2008) [PubMed] [Europe PMC] [Abstract]
  22. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
  23. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  24. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  25. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  26. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  27. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  28. "Protein kinase C-related kinase targets nuclear localization signals in a subset of class IIa histone deacetylases."FEBS Lett. 584:1103-1110(2010) [PubMed] [Europe PMC] [Abstract]
  29. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  30. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  31. "The Rho target PRK2 regulates apical junction formation in human bronchial epithelial cells."Mol. Cell. Biol. 31:81-91(2011) [PubMed] [Europe PMC] [Abstract]
  32. "Regulatory domain selectivity in the cell-type specific PKN-dependence of cell migration."PLoS ONE 6:E21732-E21732(2011) [PubMed] [Europe PMC] [Abstract]
  33. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]