≡

Cytokeratin 8 (CK8)

[Edit]

K8; CARD2; KRT8; CYK8; K2C8; KO; Keratin 8; Keratin, type II cytoskeletal 8

Cytokeratin 8 (CK8)
CCC C025
HGNC 6446
MGI 96705
PubMed 15368451
RGD 2984
UniProt P05787
Wiki Keratin_8

Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells.

This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

The human and mouse CK8 share about 82% identity in the N-terminal head domain, 95% identity in the alpha helical rod domain, and 67.5% identity in the C-terminal tail.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC025Hu01 Recombinant Cytokeratin 8 (CK8) Positive Control; Immunogen; SDS-PAGE; WB.
RPC025Hu02 Recombinant Cytokeratin 8 (CK8) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC025Hu01 Polyclonal Antibody to Cytokeratin 8 (CK8) WB,IHC,ICC/IF; FCM
MAC025Hu22 Monoclonal Antibody to Cytokeratin 8 (CK8) WB; IHC; ICC; IP.
LAC025Hu71 Biotin-Linked Polyclonal Antibody to Cytokeratin 8 (CK8) WB; IHC; ICC.
LAC025Hu81 FITC-Linked Polyclonal Antibody to Cytokeratin 8 (CK8) WB; IHC; ICC; IF.
MAC025Hu21 Monoclonal Antibody to Cytokeratin 8 (CK8) WB; IHC; ICC; IP.
MAC025Hu23 Monoclonal Antibody to Cytokeratin 8 (CK8) WB; IHC; ICC; IP.
PAC025Hu02 Polyclonal Antibody to Cytokeratin 8 (CK8) WB; IHC; ICC; IP.
Assay Kits SEC025Hu ELISA Kit for Cytokeratin 8 (CK8) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cytokeratin 8 (CK8) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cytokeratin 8 (CK8) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cytokeratin 8 (CK8) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Cytokeratin 8 (CK8) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cytokeratin 8 (CK8) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC025Ra01 Recombinant Cytokeratin 8 (CK8) Positive Control; Immunogen; SDS-PAGE; WB.
RPC025Ra02 Recombinant Cytokeratin 8 (CK8) Positive Control; Immunogen; SDS-PAGE; WB.
RPC025Ra03 Recombinant Cytokeratin 8 (CK8) Positive Control; Immunogen; SDS-PAGE; WB.
RPC025Ra04 Recombinant Cytokeratin 8 (CK8) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC025Ra01 Polyclonal Antibody to Cytokeratin 8 (CK8) WB; IHC; ICC; IP.
PAC025Ra02 Polyclonal Antibody to Cytokeratin 8 (CK8) WB; IHC; ICC; IP.
LAC025Ra71 Biotin-Linked Polyclonal Antibody to Cytokeratin 8 (CK8) WB; IHC; ICC.
PAC025Ra03 Polyclonal Antibody to Cytokeratin 8 (CK8) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Cytokeratin 8 (CK8) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cytokeratin 8 (CK8) ELISA Kit Customized Service Offer
  1. "Organization and sequence of the human gene encoding cytokeratin 8."Gene 86:241-249(1990) [PubMed] [Europe PMC] [Abstract]
  2. "Cloning and sequence of cDNA for human placental cytokeratin 8. Regulation of the mRNA in trophoblastic cells by cAMP."Mol. Endocrinol. 4:370-374(1990) [PubMed] [Europe PMC] [Abstract]
  3. "Embryonic simple epithelial keratins 8 and 18: chromosomal location emphasizes difference from other keratin pairs."New Biol. 2:464-478(1990) [PubMed] [Europe PMC] [Abstract]
  4. "Phosphorylation of human keratin 8 in vivo at conserved head domain serine 23 and at epidermal growth factor-stimulated tail domain serine 431."J. Biol. Chem. 272:7556-7564(1997) [PubMed] [Europe PMC] [Abstract]
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  6. "The finished DNA sequence of human chromosome 12." Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  8. "Posttranslational regulation of keratins: degradation of mouse and human keratins 18 and 8."Mol. Cell. Biol. 9:1553-1565(1989) [PubMed] [Europe PMC] [Abstract]
  9. "Cytokeratin expression in simple epithelia. III. Detection of mRNAs encoding human cytokeratins nos. 8 and 18 in normal and tumor cells by hybridization with cDNA sequences in vitro and in situ."Differentiation 33:69-85(1986) [PubMed] [Europe PMC] [Abstract]
  10. "A two-dimensional gel database of human colon carcinoma proteins."Electrophoresis 18:605-613(1997) [PubMed] [Europe PMC] [Abstract]
  11. "Characterization and dynamics of O-linked glycosylation of human cytokeratin 8 and 18."J. Biol. Chem. 267:3901-3906(1992) [PubMed] [Europe PMC] [Abstract]
  12. "PKC epsilon-related kinase associates with and phosphorylates cytokeratin 8 and 18."J. Cell Biol. 117:583-593(1992) [PubMed] [Europe PMC] [Abstract]
  13. "An immunohistological study of cytokeratin 20 in human and mammalian oral epithelium."Arch. Oral Biol. 45:879-887(2000) [PubMed] [Europe PMC] [Abstract]
  14. "Dissection of protein linkage between keratins and pinin, a protein with dual location at desmosome-intermediate filament complex and in the nucleus."J. Biol. Chem. 275:14910-14915(2000) [PubMed] [Europe PMC] [Abstract]
  15. "The intermediate filament protein keratin 8 is a novel cytoplasmic substrate for c-Jun N-terminal kinase."J. Biol. Chem. 277:10767-10774(2002) [PubMed] [Europe PMC] [Abstract]
  16. "Keratin 8 phosphorylation by p38 kinase regulates cellular keratin filament reorganization: modulation by a keratin 1-like disease causing mutation."J. Biol. Chem. 277:10775-10782(2002) [PubMed] [Europe PMC] [Abstract]
  17. "Identification of trichoplein, a novel keratin filament-binding protein."J. Cell Sci. 118:1081-1090(2005) [PubMed] [Europe PMC] [Abstract]
  18. "Specific interaction of the actin-binding domain of dystrophin with intermediate filaments containing keratin 19."Mol. Biol. Cell 16:4280-4293(2005) [PubMed] [Europe PMC] [Abstract]
  19. "Proteomic profiling of cellular proteins interacting with the hepatitis C virus core protein."Proteomics 5:2227-2237(2005) [PubMed] [Europe PMC] [Abstract]
  20. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  21. "Keratin 20 serine 13 phosphorylation is a stress and intestinal goblet cell marker."J. Biol. Chem. 281:16453-16461(2006) [PubMed] [Europe PMC] [Abstract]
  22. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  23. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  24. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  25. "APE1/Ref-1 interacts with NPM1 within nucleoli and plays a role in the rRNA quality control process."Mol. Cell. Biol. 29:1834-1854(2009) [PubMed] [Europe PMC] [Abstract]
  26. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  27. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  28. "O-GlcNAcylation determines the solubility, filament organization, and stability of keratins 8 and 18."J. Biol. Chem. 285:34062-34071(2010) [PubMed] [Europe PMC] [Abstract]
  29. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  30. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  31. "The first identification of lysine malonylation substrates and its regulatory enzyme."Mol. Cell. Proteomics 10:M111.012658.01-M111.012658.12(2011) [PubMed] [Europe PMC] [Abstract]
  32. "G protein-coupled estrogen receptor 1/G protein-coupled receptor 30 localizes in the plasma membrane and traffics intracellularly on cytokeratin intermediate filaments."Mol. Pharmacol. 79:400-410(2011) [PubMed] [Europe PMC] [Abstract]
  33. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  34. "Characterization of O-GlcNAc cycling and proteomic identification of differentially O-GlcNAcylated proteins during G1/S transition."Biochim. Biophys. Acta 1820:1839-1848(2012) [PubMed] [Europe PMC] [Abstract]
  35. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  36. "Keratin 8 and 18 mutations are risk factors for developing liver disease of multiple etiologies."Proc. Natl. Acad. Sci. U.S.A. 100:6063-6068(2003) [PubMed] [Europe PMC] [Abstract]