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Protein O-Fucosyltransferase 1 (POFUT1)

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O-FUT; O-Fuc-T; FUT12; GDP-Fucose Protein O-Fucosyltransferase 1; Peptide-O-fucosyltransferase 1

Protein O-Fucosyltransferase 1 (POFUT1)
CCC C072
HGNC 14988
MGI 2153207
PubMed 23684010
RGD 1303001
UniProt Q9H488
Wiki POFUT1

-fucosyltransferase 1 (POFUT1) is an enzyme responsible for adding fucose sugars in O linkage to serine or threonine residues between the second and third conserved cysteines in EGF-like repeats on the Notch protein. The protein is an inverting glycosyltransferase, which means that the enzyme uses GDP-beta-L-fucose as a donor substrate and transfers the fucose in O linkage to the protein producing fucose-alpha-O-serine/threonine.

When the gene for POFUT1 is knocked out, or the expression is decreased to very low levels, all Notch signaling is destroyed, which means that fucose on Notch is absolutely essential for Notch function. Why this is the case is not yet well understood.Almost all glycosyltransferases reside in the Golgi apparatus.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC072Hu01 Recombinant Protein O-Fucosyltransferase 1 (POFUT1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC072Hu01 Polyclonal Antibody to Protein O-Fucosyltransferase 1 (POFUT1) WB; IHC
Assay Kits n/a CLIA Kit for Protein O-Fucosyltransferase 1 (POFUT1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein O-Fucosyltransferase 1 (POFUT1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC072Mu01 Recombinant Protein O-Fucosyltransferase 1 (POFUT1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC072Mu01 Polyclonal Antibody to Protein O-Fucosyltransferase 1 (POFUT1) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Protein O-Fucosyltransferase 1 (POFUT1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein O-Fucosyltransferase 1 (POFUT1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Protein O-Fucosyltransferase 1 (POFUT1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Protein O-Fucosyltransferase 1 (POFUT1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Protein O-Fucosyltransferase 1 (POFUT1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Protein O-Fucosyltransferase 1 (POFUT1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein O-Fucosyltransferase 1 (POFUT1) ELISA Kit Customized Service Offer
  1. "Modification of epidermal growth factor-like repeats with O-fucose: molecular cloning and expression of a novel GDP-fucose protein O-fucosyltransferase."J. Biol. Chem. 276:40338-40345(2001) [PubMed] [Europe PMC] [Abstract]
  2. "Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1."DNA Res. 3:17-24(1996) [PubMed] [Europe PMC] [Abstract]
  3. "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The DNA sequence and comparative analysis of human chromosome 20." Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "O-linked fucose and other post-translational modifications unique to EGF modules."Glycobiology 3:219-224(1993) [PubMed] [Europe PMC] [Abstract]
  8. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
  9. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  10. "Mutations in POFUT1, encoding protein O-fucosyltransferase 1, cause generalized Dowling-Degos disease."Am. J. Hum. Genet. 92:895-903(2013) [PubMed] [Europe PMC] [Abstract]