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Poly A Specific Ribonuclease (PARN)

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DAN; Deadenylation Nuclease; Deadenylating nuclease; Polyadenylate-specific ribonuclease

Poly A Specific Ribonuclease (PARN)
CCC C176
HGNC 8609
MGI 1921358
PubMed 22834816
RGD 1565449
UniProt O95453
Wiki PARN
Poly A Specific Ribonuclease is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Poly(A)-specific ribonuclease PARN protein appears to be localized in both the nucleus and the cytoplasm. It is not stably associated with polysomes or ribosomal subunits.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC176Hu01 Recombinant Poly A Specific Ribonuclease (PARN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC176Hu01 Polyclonal Antibody to Poly A Specific Ribonuclease (PARN) WB; IHC; ICC; IP.
MAC176Hu22 Monoclonal Antibody to Poly A Specific Ribonuclease (PARN) WB; IHC; ICC; IP.
Assay Kits SEC176Hu ELISA Kit for Poly A Specific Ribonuclease (PARN) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Poly A Specific Ribonuclease (PARN) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Poly A Specific Ribonuclease (PARN) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Poly A Specific Ribonuclease (PARN) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Poly A Specific Ribonuclease (PARN) CLIA Kit Customized Service Offer
n/a ELISA Kit for Poly A Specific Ribonuclease (PARN) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Poly A Specific Ribonuclease (PARN) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Poly A Specific Ribonuclease (PARN) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Poly A Specific Ribonuclease (PARN) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Poly A Specific Ribonuclease (PARN) CLIA Kit Customized Service Offer
n/a ELISA Kit for Poly A Specific Ribonuclease (PARN) ELISA Kit Customized Service Offer
  1. "The deadenylating nuclease (DAN) is involved in poly(A) tail removal during the meiotic maturation of Xenopus oocytes."EMBO J. 17:5427-5437(1998) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The sequence and analysis of duplication-rich human chromosome 16." Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The human gene for the poly(A)-specific ribonuclease (PARN) maps to 16p13 and has a truncated copy in the Prader-Willi/Angelman syndrome region on 15q11-->q13."Cytogenet. Cell Genet. 87:125-131(1999) [PubMed] [Europe PMC] [Abstract]
  6. "A 54-kDa fragment of the Poly(A)-specific ribonuclease is an oligomeric, processive, and cap-interacting Poly(A)-specific 3' exonuclease."J. Biol. Chem. 275:24222-24230(2000) [PubMed] [Europe PMC] [Abstract]
  7. "Interaction between a poly(A)-specific ribonuclease and the 5' cap influences mRNA deadenylation rates in vitro."Mol. Cell 5:479-488(2000) [PubMed] [Europe PMC] [Abstract]
  8. "The mRNA cap structure stimulates rate of poly(A) removal and amplifies processivity of degradation."J. Biol. Chem. 276:27923-27929(2001) [PubMed] [Europe PMC] [Abstract]
  9. "Identification of the active site of poly(A)-specific ribonuclease by site-directed mutagenesis and Fe(2+)-mediated cleavage."J. Biol. Chem. 277:5982-5987(2002) [PubMed] [Europe PMC] [Abstract]
  10. "Functional proteomic analysis of human nucleolus."Mol. Biol. Cell 13:4100-4109(2002) [PubMed] [Europe PMC] [Abstract]
  11. "Nonsense-mediated mRNA decay in mammalian cells involves decapping, deadenylating, and exonucleolytic activities."Mol. Cell 12:675-687(2003) [PubMed] [Europe PMC] [Abstract]
  12. "Tristetraprolin and its family members can promote the cell-free deadenylation of AU-rich element-containing mRNAs by poly(A) ribonuclease."Mol. Cell. Biol. 23:3798-3812(2003) [PubMed] [Europe PMC] [Abstract]
  13. "A KH domain RNA binding protein, KSRP, promotes ARE-directed mRNA turnover by recruiting the degradation machinery."Mol. Cell 14:571-583(2004) [PubMed] [Europe PMC] [Abstract]
  14. "Coordination of divalent metal ions in the active site of poly(A)-specific ribonuclease."J. Biol. Chem. 279:48702-48706(2004) [PubMed] [Europe PMC] [Abstract]
  15. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  16. "CUG-BP binds to RNA substrates and recruits PARN deadenylase."RNA 12:1084-1091(2006) [PubMed] [Europe PMC] [Abstract]
  17. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  18. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  19. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  20. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  21. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  22. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  23. "Zinc-finger antiviral protein inhibits HIV-1 infection by selectively targeting multiply spliced viral mRNAs for degradation."Proc. Natl. Acad. Sci. U.S.A. 108:15834-15839(2011) [PubMed] [Europe PMC] [Abstract]
  24. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  25. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  26. "Structural insight into poly(A) binding and catalytic mechanism of human PARN."EMBO J. 24:4082-4093(2005) [PubMed] [Europe PMC] [Abstract]
  27. "DNA damage activates a spatially distinct late cytoplasmic cell-cycle checkpoint network controlled by MK2-mediated RNA stabilization."Mol. Cell 40:34-49(2010) [PubMed] [Europe PMC] [Abstract]