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Cortactin (CTTN)

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EMS1; Cortical Actin binding Protein; Src substrate cortactin; Amplaxin; Oncogene EMS1

Cortactin (CTTN)
CCC C234
HGNC 3338
MGI 99695
PubMed 11607842
RGD 619839
UniProt Q14247
Wiki Cortactin

Cortactin is a monomeric protein located in the cytoplasm of cells that can be activated by external stimuli to promote polymerization and rearrangement of the actin cytoskeleton, especially the actin cortex around the cellular periphery. It is present in all cell types. When activated, it will recruit Arp2/3 complex proteins to existing actin microfilaments, facilitating and stabilizing nucleation sites for actin branching. Cortactin is important in promoting lamellipodia formation, invadopodia formation, cell migration, and endocytosis.Cortactin is a thin, elongated monomer that consists of an amino-terminal acidic (NTA) region; 37-residue-long segments that are highly conserved among cortactin proteins of all species and which are repeated up to 6.5 times in tandem (ldquo;cortactin repeats rdquo;); a proline-rich region; and an SH3 domain.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cortactin (CTTN) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cortactin (CTTN) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cortactin (CTTN) Polyclonal Antibody Customized Service Offer
Assay Kits SEC234Hu ELISA Kit for Cortactin (CTTN) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC234Mu01 Recombinant Cortactin (CTTN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC234Mu01 Polyclonal Antibody to Cortactin (CTTN) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Cortactin (CTTN) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cortactin (CTTN) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC234Ra01 Recombinant Cortactin (CTTN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC234Ra01 Polyclonal Antibody to Cortactin (CTTN) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Cortactin (CTTN) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cortactin (CTTN) ELISA Kit Customized Service Offer
  1. "Identification and cloning of two overexpressed genes, U21B31/PRAD1 and EMS1, within the amplified chromosome 11q13 region in human carcinomas."Oncogene 7:355-361(1992) [PubMed] [Europe PMC] [Abstract]
  2. "The product of the EMS1 gene, amplified and overexpressed in human carcinomas, is homologous to a v-src substrate and is located in cell-substratum contact sites."Mol. Cell. Biol. 13:2891-2898(1993) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Human chromosome 11 DNA sequence and analysis including novel gene identification." Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Growth factor-dependent phosphorylation of the actin-binding protein cortactin is mediated by the cytoplasmic tyrosine kinase FER."J. Biol. Chem. 273:23542-23548(1998) [PubMed] [Europe PMC] [Abstract]
  7. "Novel interaction of cortactin with endothelial cell myosin light chain kinase."Biochem. Biophys. Res. Commun. 298:511-519(2002) [PubMed] [Europe PMC] [Abstract]
  8. "Tyrosine phosphorylation of Kv1.2 modulates its interaction with the actin-binding protein cortactin."J. Biol. Chem. 277:38596-38606(2002) [PubMed] [Europe PMC] [Abstract]
  9. "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry."Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract]
  10. "Identification of a binding partner for the endothelial cell surface proteins TEM7 and TEM7R."Cancer Res. 64:8507-8511(2004) [PubMed] [Europe PMC] [Abstract]
  11. "The proto-oncogene Fgr regulates cell migration and this requires its plasma membrane localization."Exp. Cell Res. 302:253-269(2005) [PubMed] [Europe PMC] [Abstract]
  12. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
  13. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  14. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  15. "Toward a global characterization of the phosphoproteome in prostate cancer cells: identification of phosphoproteins in the LNCaP cell line."Electrophoresis 28:2027-2034(2007) [PubMed] [Europe PMC] [Abstract]
  16. "An essential role for cortactin in the modulation of the potassium channel Kv1.2."Proc. Natl. Acad. Sci. U.S.A. 104:17412-17417(2007) [PubMed] [Europe PMC] [Abstract]
  17. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
  18. "Phosphoproteome of resting human platelets."J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
  19. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  20. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  21. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  22. "Dynamic microtubules regulate dendritic spine morphology and synaptic plasticity."Neuron 61:85-100(2009) [PubMed] [Europe PMC] [Abstract]
  23. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  24. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  25. "Abl tyrosine kinase phosphorylates nonmuscle Myosin light chain kinase to regulate endothelial barrier function."Mol. Biol. Cell 21:4042-4056(2010) [PubMed] [Europe PMC] [Abstract]
  26. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  27. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  28. "The immunoinhibitory adapter protein SRC homology domain 3 lymphocyte protein 2 (SLy2) regulates actin dynamics and B cell spreading."J. Biol. Chem. 286:13489-13501(2011) [PubMed] [Europe PMC] [Abstract]
  29. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  30. "Cortactin controls surface expression of the voltage-gated potassium channel K(V)10.1."J. Biol. Chem. 287:44151-44163(2012) [PubMed] [Europe PMC] [Abstract]
  31. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  32. "Solution structures of the SH3 domain of human Src substrate cortactin."Submitted (NOV-2005) to the PDB data bank
  33. "Targeting AMAP1 and cortactin binding bearing an atypical src homology 3/proline interface for prevention of breast cancer invasion and metastasis."Proc. Natl. Acad. Sci. U.S.A. 103:7036-7041(2006) [PubMed] [Europe PMC] [Abstract]