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Calpastatin (CAST)

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BS17; Calpain inhibitor; Sperm BS-17 component

Calpastatin (CAST)
CCC C416
HGNC 1515
MGI 1098236
PubMed 12684003
RGD 2278
UniProt P20810
Wiki Calpastatin

Calpastatin is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation.

The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length natures of only some have been determined.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC416Hu01 Recombinant Calpastatin (CAST) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies n/a Monoclonal Antibody to Calpastatin (CAST) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Calpastatin (CAST) Polyclonal Antibody Customized Service Offer
Assay Kits SEC416Hu ELISA Kit for Calpastatin (CAST) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC416Mu01 Recombinant Calpastatin (CAST) Positive Control; Immunogen; SDS-PAGE; WB.
RPC416Mu02 Recombinant Calpastatin (CAST) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC416Mu01 Polyclonal Antibody to Calpastatin (CAST) WB; IHC; ICC; IP.
PAC416Mu02 Polyclonal Antibody to Calpastatin (CAST) WB; IHC; ICC; IP.
Assay Kits SEC416Mu ELISA Kit for Calpastatin (CAST) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Calpastatin (CAST) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Calpastatin (CAST) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Calpastatin (CAST) Polyclonal Antibody Customized Service Offer
Assay Kits SEC416Ra ELISA Kit for Calpastatin (CAST) Enzyme-linked immunosorbent assay for Antigen Detection.
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  2. "Cloning and characterization of a novel isoform of calpastatin in human adult testis."Acta Pharmacol. Sin. 23:450-454(2002) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The DNA sequence and comparative analysis of human chromosome 5." Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
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  6. "The full-ORF clone resource of the German cDNA consortium."BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
  7. "Molecular diversity in amino-terminal domains of human calpastatin by exon skipping."J. Biol. Chem. 267:8437-8442(1992) [PubMed] [Europe PMC] [Abstract]
  8. "Inhibition of calpain by a synthetic oligopeptide corresponding to an exon of the human calpastatin gene."J. Biol. Chem. 264:18866-18869(1989) [PubMed] [Europe PMC] [Abstract]
  9. "Characterization of a functional domain of human calpastatin."Biochem. Biophys. Res. Commun. 166:1485-1493(1990) [PubMed] [Europe PMC] [Abstract]
  10. "Calpastatin gene in human testis."Biochem. Mol. Biol. Int. 33:245-252(1994) [PubMed] [Europe PMC] [Abstract]
  11. "Detection and expression of a cDNA clone that encodes a polypeptide containing two inhibitory domains of human calpastatin and its recognition by rheumatoid arthritis sera."J. Clin. Invest. 95:1891-1896(1995) [PubMed] [Europe PMC] [Abstract]
  12. "Phosphorylation and subcellular distribution of calpastatin in human hematopoietic system cells."J. Biol. Chem. 266:3968-3972(1991) [PubMed] [Europe PMC] [Abstract]
  13. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  14. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  15. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  16. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  17. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  18. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]