≡

Vasodilator Stimulated Phosphoprotein (VASP)

[Edit]

Vasodilator Stimulated Phosphoprotein (VASP)
CCC C603
HGNC 12652
MGI 109268
PubMed 19825941
RGD 1311542
UniProt P50552
Wiki VASP
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC603Hu01 Recombinant Vasodilator Stimulated Phosphoprotein (VASP) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC603Hu01 Polyclonal Antibody to Vasodilator Stimulated Phosphoprotein (VASP) WB; IHC; ICC; IP.
MAC603Hu22 Monoclonal Antibody to Vasodilator Stimulated Phosphoprotein (VASP) WB; IHC; ICC; IP.
Assay Kits SEC603Hu ELISA Kit for Vasodilator Stimulated Phosphoprotein (VASP) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Vasodilator Stimulated Phosphoprotein (VASP) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Vasodilator Stimulated Phosphoprotein (VASP) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Vasodilator Stimulated Phosphoprotein (VASP) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Vasodilator Stimulated Phosphoprotein (VASP) CLIA Kit Customized Service Offer
n/a ELISA Kit for Vasodilator Stimulated Phosphoprotein (VASP) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC603Ra01 Recombinant Vasodilator Stimulated Phosphoprotein (VASP) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC603Ra01 Polyclonal Antibody to Vasodilator Stimulated Phosphoprotein (VASP) WB; IHC; ICC; IP.
MAC603Ra21 Monoclonal Antibody to Vasodilator Stimulated Phosphoprotein (VASP) WB; IHC; ICC; IP.
Assay Kits SEC603Ra ELISA Kit for Vasodilator Stimulated Phosphoprotein (VASP) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "Molecular cloning, structural analysis and functional expression of the proline-rich focal adhesion and microfilament-associated protein VASP."EMBO J. 14:19-27(1995) [PubMed] [Europe PMC] [Abstract]
  2. "Role of proteins of the Ena/VASP family in actin-based motility of Listeria monocytogenes."J. Cell Biol. 144:1245-1258(1999) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
  6. "Cloning of the VASP (vasodilator-stimulated phosphoprotein) genes in human and mouse: structure, sequence, and chromosomal localization."Genomics 36:227-233(1996) [PubMed] [Europe PMC] [Abstract]
  7. "cAMP- and cGMP-dependent protein kinase phosphorylation sites of the focal adhesion vasodilator-stimulated phosphoprotein (VASP) in vitro and in intact human platelets."J. Biol. Chem. 269:14509-14517(1994) [PubMed] [Europe PMC] [Abstract]
  8. "Phosphorylation of focal adhesion vasodilator-stimulated phosphoprotein at Ser157 in intact human platelets correlates with fibrinogen receptor inhibition."Eur. J. Biochem. 225:21-27(1994) [PubMed] [Europe PMC] [Abstract]
  9. "The proline-rich focal adhesion and microfilament protein VASP is a ligand for profilins."EMBO J. 14:1583-1589(1995) [PubMed] [Europe PMC] [Abstract]
  10. "The EVH2 domain of the vasodilator-stimulated phosphoprotein mediates tetramerization, F-actin binding, and actin bundle formation."J. Biol. Chem. 274:23549-23557(1999) [PubMed] [Europe PMC] [Abstract]
  11. "Characterization of the interaction between zyxin and members of the Ena/vasodilator-stimulated phosphoprotein family of proteins."J. Biol. Chem. 275:22503-22511(2000) [PubMed] [Europe PMC] [Abstract]
  12. "LPP, an actin cytoskeleton protein related to zyxin, harbors a nuclear export signal and transcriptional activation capacity."Mol. Biol. Cell 11:117-129(2000) [PubMed] [Europe PMC] [Abstract]
  13. "Lamellipodin, an Ena/VASP ligand, is implicated in the regulation of lamellipodial dynamics."Dev. Cell 7:571-583(2004) [PubMed] [Europe PMC] [Abstract]
  14. "Vasodilator-stimulated phosphoprotein is a substrate for protein kinase C."FEBS Lett. 556:211-215(2004) [PubMed] [Europe PMC] [Abstract]
  15. "Ena/VASP proteins enhance actin polymerization in the presence of barbed end capping proteins."J. Biol. Chem. 280:28653-28662(2005) [PubMed] [Europe PMC] [Abstract]
  16. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
  17. "Vasodilator-stimulated phosphoprotein (VASP) is phosphorylated on Ser 157 by protein kinase C-dependent and -independent mechanisms in thrombin-stimulated human platelets."Biochem. J. 393:555-564(2006) [PubMed] [Europe PMC] [Abstract]
  18. "Unusual splicing events result in distinct Xin isoforms that associate differentially with filamin c and Mena/VASP."Exp. Cell Res. 312:2154-2167(2006) [PubMed] [Europe PMC] [Abstract]
  19. "AMP-activated protein kinase impairs endothelial actin cytoskeleton assembly by phosphorylating vasodilator-stimulated phosphoprotein."J. Biol. Chem. 282:4601-4612(2007) [PubMed] [Europe PMC] [Abstract]
  20. "Carbon monoxide and nitric oxide mediate cytoskeletal reorganization in microvascular cells via vasodilator-stimulated phosphoprotein phosphorylation: evidence for blunted responsiveness in diabetes."Diabetes 57:2488-2494(2008) [PubMed] [Europe PMC] [Abstract]
  21. "Phosphoproteome of resting human platelets."J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
  22. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  23. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  24. "Pyrin and ASC co-localize to cellular sites that are rich in polymerizing actin."Exp. Biol. Med. (Maywood) 234:40-52(2009) [PubMed] [Europe PMC] [Abstract]
  25. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  26. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  27. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  28. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  29. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  30. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
  31. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  32. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  33. "Dual epitope recognition by the VASP EVH1 domain modulates polyproline ligand specificity and binding affinity."EMBO J. 19:4903-4914(2000) [PubMed] [Europe PMC] [Abstract]
  34. "The VASP tetramerization domain is a right-handed coiled coil based on a 15-residue repeat."Proc. Natl. Acad. Sci. U.S.A. 101:17027-17032(2004) [PubMed] [Europe PMC] [Abstract]
  35. "Structural basis for the recruitment of profilin-actin complexes during filament elongation by Ena/VASP."EMBO J. 26:4597-4606(2007) [PubMed] [Europe PMC] [Abstract]
  36. "Modulation of actin structure and function by phosphorylation of Tyr-53 and profilin binding."Proc. Natl. Acad. Sci. U.S.A. 105:11748-11753(2008) [PubMed] [Europe PMC] [Abstract]