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Neutrophil Cytosolic Factor 1 (NCF1)

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NCF1A; NOXO2; SH3PXD1A; p47-phox; NCF-47K; 47 kDa autosomal chronic granulomatous disease protein; Neutrophil NADPH oxidase factor 1; Nox-organizing protein 2

Neutrophil Cytosolic Factor 1 (NCF1)
CCC C677
HGNC 7660
MGI 97283
PubMed 15936744
UniProt P14598
Wiki NCF1
Neutrophil cytosol factor 1 is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease.
A predominant transcriptional start site was identified 21 nucleotides upstream of the translation initiation codon. Transient transfections of HL60 human myeloid cells were performed with a series of 5-prime-deletion p47-phox-luciferase reporter constructs to identify the gene promoter sequences. The -224 and -86 constructs had the strongest p47-phox promoter activity, whereas the -46 construct showed a major reduction in activity and the -36 construct a complete loss of activity.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC677Hu01 Recombinant Neutrophil Cytosolic Factor 1 (NCF1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC677Hu01 Polyclonal Antibody to Neutrophil Cytosolic Factor 1 (NCF1) WB; IHC; ICC; IP.
MAC677Hu21 Monoclonal Antibody to Neutrophil Cytosolic Factor 1 (NCF1) WB; IHC; ICC; IP.
Assay Kits SEC677Hu ELISA Kit for Neutrophil Cytosolic Factor 1 (NCF1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Neutrophil Cytosolic Factor 1 (NCF1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Neutrophil Cytosolic Factor 1 (NCF1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Neutrophil Cytosolic Factor 1 (NCF1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Neutrophil Cytosolic Factor 1 (NCF1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Neutrophil Cytosolic Factor 1 (NCF1) ELISA Kit Customized Service Offer
  1. "Cloning of the cDNA and functional expression of the 47-kilodalton cytosolic component of human neutrophil respiratory burst oxidase."Proc. Natl. Acad. Sci. U.S.A. 86:7195-7199(1989) [PubMed] [Europe PMC] [Abstract]
  2. ErratumProc. Natl. Acad. Sci. U.S.A. 86:9563-9563(1989)
  3. "Recombinant 47-kilodalton cytosol factor restores NADPH oxidase in chronic granulomatous disease."Science 245:409-412(1989) [PubMed] [Europe PMC] [Abstract]
  4. "Characterization of the 47-kilodalton autosomal chronic granulomatous disease protein: tissue-specific expression and transcriptional control by retinoic acid."Mol. Cell. Biol. 10:5388-5396(1990) [PubMed] [Europe PMC] [Abstract]
  5. "A p47-phox pseudogene carries the most common mutation causing p47-phox-deficient chronic granulomatous disease."J. Clin. Invest. 100:1907-1918(1997) [PubMed] [Europe PMC] [Abstract]
  6. "Genomic structure of the human p47-phox (NCF1) gene."Blood Cells Mol. Dis. 26:37-46(2000) [PubMed] [Europe PMC] [Abstract]
  7. "TNFalpha activates c-jun amino terminal kinase through p47(phox)."Exp. Cell Res. 272:62-74(2002) [PubMed] [Europe PMC] [Abstract]
  8. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  9. "The DNA sequence of human chromosome 7." Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
  10. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  11. "Comparison of the positional cloning methods used to isolate the BRCA1 gene." Hum. Mol. Genet. 4:1259-1266(1995) [PubMed] [Europe PMC] [Abstract]
  12. "Autosomal recessive chronic granulomatous disease caused by deletion at a dinucleotide repeat."Proc. Natl. Acad. Sci. U.S.A. 88:2753-2757(1991) [PubMed] [Europe PMC] [Abstract]
  13. "The phosphorylation of the respiratory burst oxidase component p47phox during neutrophil activation. Phosphorylation of sites recognized by protein kinase C and by proline-directed kinases."J. Biol. Chem. 269:23431-23436(1994) [PubMed] [Europe PMC] [Abstract]
  14. "Interaction of human neutrophil flavocytochrome b with cytosolic proteins: transferred-NOESY NMR studies of a gp91phox C-terminal peptide bound to p47phox."Biochem. J. 325:249-257(1997) [PubMed] [Europe PMC] [Abstract]
  15. "Involvement of TRAF4 in oxidative activation of c-Jun N-terminal kinase."J. Biol. Chem. 277:28051-28057(2002) [PubMed] [Europe PMC] [Abstract]
  16. "Novel human homologues of p47phox and p67phox participate in activation of superoxide-producing NADPH oxidases."J. Biol. Chem. 278:25234-25246(2003) [PubMed] [Europe PMC] [Abstract]
  17. "TRAF4 acts as a silencer in TLR-mediated signaling through the association with TRAF6 and TRIF."Eur. J. Immunol. 35:2477-2485(2005) [PubMed] [Europe PMC] [Abstract]
  18. "Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening."BMC Immunol. 10:53-53(2009) [PubMed] [Europe PMC] [Abstract]
  19. "Alternative splicing of ADAM15 regulates its interactions with cellular SH3 proteins."J. Cell. Biochem. 108:877-885(2009) [PubMed] [Europe PMC] [Abstract]
  20. "Regulation of TNF-induced oxygen radical production in human neutrophils: role of delta-PKC."J. Leukoc. Biol. 87:153-164(2010) [PubMed] [Europe PMC] [Abstract]
  21. "Solution structure of the PX domain, a target of the SH3 domain."Nat. Struct. Biol. 8:526-530(2001) [PubMed] [Europe PMC] [Abstract]
  22. "Diverse recognition of non-PxxP peptide ligands by the SH3 domains from p67(phox), Grb2 and Pex13p."EMBO J. 21:4268-4276(2002) [PubMed] [Europe PMC] [Abstract]
  23. "Binding of the PX domain of p47(phox) to phosphatidylinositol 3,4-bisphosphate and phosphatidic acid is masked by an intramolecular interaction."EMBO J. 21:5057-5068(2002) [PubMed] [Europe PMC] [Abstract]
  24. "Molecular basis of phosphorylation-induced activation of the NADPH oxidase."Cell 113:343-355(2003) [PubMed] [Europe PMC] [Abstract]
  25. "A molecular mechanism for autoinhibition of the tandem SH3 domains of p47phox, the regulatory subunit of the phagocyte NADPH oxidase."Genes Cells 9:443-456(2004) [PubMed] [Europe PMC] [Abstract]
  26. "Effects of p47phox C terminus phosphorylations on binding interactions with p40phox and p67phox. Structural and functional comparison of p40phox and p67phox SH3 domains."J. Biol. Chem. 280:13752-13761(2005) [PubMed] [Europe PMC] [Abstract]
  27. "NMR solution structure of the tandem Src homology 3 domains of p47phox complexed with a p22phox-derived proline-rich peptide."J. Biol. Chem. 281:3660-3668(2006) [PubMed] [Europe PMC] [Abstract]
  28. "Autosomal recessive chronic granulomatous disease caused by defects in NCF1, the gene encoding the phagocyte p47-phox: mutations not arising in the NCF1 pseudogenes."Blood 97:305-311(2001) [PubMed] [Europe PMC] [Abstract]
  29. "Clinical, functional, and genetic characterization of chronic granulomatous disease in 89 Turkish patients."J. Allergy Clin. Immunol. 132:1156-1163(2013) [PubMed] [Europe PMC] [Abstract]