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Cytoplasmic Protein NCK1 (NCK1)

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NCKalpha; NCKa; NCK adaptor protein 1; Non Catalytic Region Of Tyrosine Kinase Adaptor Protein 1; SH2/SH3 adaptor protein NCK-alpha

Cytoplasmic Protein NCK1 (NCK1)
CCC C712
HGNC 7664
MGI 109601
PubMed 23280595
RGD 1310688
UniProt P16333
Wiki NCK1

The Cytoplasmic protein NCK1 Protein belongs to the adaptor family of proteins.The Nck family has two known members in human cells.(Nck-1/Nckalpha and NcK2/NcKbeta), two in mouse cells(mNckalpha and mNckbeta/Grb4) and one in drosophila( Dock means dreadlocks-ortholog). The two murine gene products exhibits 68% aminoacid identity to one another,with most of the sequence variation being located to the linker regions between the SH3 and SH2 domains, and are 96% identical to their human counterparts. While human nck-1 gene has been localised to the 3q21 locus of chromosome 3,the nck-2 genecan be found on chromosome 2 at the 2q12 locus. The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC712Hu01 Recombinant Cytoplasmic Protein NCK1 (NCK1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC712Hu01 Polyclonal Antibody to Cytoplasmic Protein NCK1 (NCK1) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Cytoplasmic Protein NCK1 (NCK1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cytoplasmic Protein NCK1 (NCK1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cytoplasmic Protein NCK1 (NCK1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cytoplasmic Protein NCK1 (NCK1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cytoplasmic Protein NCK1 (NCK1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Cytoplasmic Protein NCK1 (NCK1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cytoplasmic Protein NCK1 (NCK1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cytoplasmic Protein NCK1 (NCK1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cytoplasmic Protein NCK1 (NCK1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cytoplasmic Protein NCK1 (NCK1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Cytoplasmic Protein NCK1 (NCK1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cytoplasmic Protein NCK1 (NCK1) ELISA Kit Customized Service Offer
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  6. "The SH2/SH3 domain-containing protein Nck is recognized by certain anti-phospholipase C-gamma 1 monoclonal antibodies, and its phosphorylation on tyrosine is stimulated by platelet-derived growth factor and epidermal growth factor treatment."Mol. Cell. Biol. 12:5843-5856(1992) [PubMed] [Europe PMC] [Abstract]
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  8. "A novel ligand for an SH3 domain of the adaptor protein Nck bears an SH2 domain and nuclear signaling motifs."Biochem. Biophys. Res. Commun. 239:488-492(1997) [PubMed] [Europe PMC] [Abstract]
  9. "Tyrosine 1213 of Flt-1 is a major binding site of Nck and SHP-2."Biochem. Biophys. Res. Commun. 246:95-99(1998) [PubMed] [Europe PMC] [Abstract]
  10. "BLNK: a central linker protein in B cell activation."Immunity 9:93-103(1998) [PubMed] [Europe PMC] [Abstract]
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  12. "Identification of Grb4/Nckbeta, a src homology 2 and 3 domain-containing adapter protein having similar binding and biological properties to Nck."J. Biol. Chem. 274:5542-5549(1999) [PubMed] [Europe PMC] [Abstract]
  13. "Identification and characterization of a new family of guanine nucleotide exchange factors for the ras-related GTPase Ral."J. Biol. Chem. 275:13406-13410(2000) [PubMed] [Europe PMC] [Abstract]
  14. "Src-induced phosphorylation of caveolin-2 on tyrosine 19. Phospho-caveolin-2 (Tyr(P)19) is localized near focal adhesions, remains associated with lipid rafts/caveolae, but no longer forms a high molecular mass hetero-oligomer with caveolin-1."J. Biol. Chem. 277:34556-34567(2002) [PubMed] [Europe PMC] [Abstract]
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  16. "Identification and functional characterization of a novel human misshapen/Nck interacting kinase-related kinase, hMINK beta."J. Biol. Chem. 279:54387-54397(2004) [PubMed] [Europe PMC] [Abstract]
  17. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  18. "Nck in a complex containing the catalytic subunit of protein phosphatase 1 regulates eukaryotic initiation factor 2alpha signaling and cell survival to endoplasmic reticulum stress."J. Biol. Chem. 281:26633-26644(2006) [PubMed] [Europe PMC] [Abstract]
  19. "Phosphorylation of Tyr1214 within VEGFR-2 triggers the recruitment of Nck and activation of Fyn leading to SAPK2/p38 activation and endothelial cell migration in response to VEGF."J. Biol. Chem. 281:34009-34020(2006) [PubMed] [Europe PMC] [Abstract]
  20. "Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7."Cell 130:837-850(2007) [PubMed] [Europe PMC] [Abstract]
  21. "Nck-1 interacts with PKR and modulates its activation by dsRNA."Biochem. Biophys. Res. Commun. 377:231-235(2008) [PubMed] [Europe PMC] [Abstract]
  22. "Phosphoproteome of resting human platelets."J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
  23. "Distinct functions of natural ADAM-15 cytoplasmic domain variants in human mammary carcinoma."Mol. Cancer Res. 6:383-394(2008) [PubMed] [Europe PMC] [Abstract]
  24. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  25. "Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening."BMC Immunol. 10:53-53(2009) [PubMed] [Europe PMC] [Abstract]
  26. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  27. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  28. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  29. "Adaptor protein Nck1 interacts with p120 Ras GTPase-activating protein and regulates its activity."Cell. Signal. 23:1651-1658(2011) [PubMed] [Europe PMC] [Abstract]
  30. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
  31. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  32. "Receptor protein tyrosine phosphatase-receptor tyrosine kinase substrate screen identifies EphA2 as a target for LAR in cell migration."Mol. Cell. Biol. 33:1430-1441(2013) [PubMed] [Europe PMC] [Abstract]
  33. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  34. "Solution structure of the SH3 domain of the human cytoplasmic protein NCK1."Submitted (NOV-2005) to the PDB data bank
  35. "Specificity determinants of a novel Nck interaction with the juxtamembrane domain of the epidermal growth factor receptor."Biochemistry 47:3096-3108(2008) [PubMed] [Europe PMC] [Abstract]