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Poly U Binding Splicing Factor 60kDa (PUF60)

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FIR; SIAHBP1; RoBPI; Fuse Binding Protein Interacting Repressor; Siah Binding Protein 1; Pyrimidine Tract Binding Splicing Factor; Ro Ribonucleoprotein Binding Protein 1

Poly U Binding Splicing Factor 60kDa (PUF60)
CCC C740
HGNC 17042
MGI 1915209
PubMed 10606266
RGD 621674
UniProt Q9UHX1
Wiki PUF60
Poly(U)-binding-splicing factor PUF60 is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE.
It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC740Hu01 Recombinant Poly U Binding Splicing Factor 60kDa (PUF60) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC740Hu01 Polyclonal Antibody to Poly U Binding Splicing Factor 60kDa (PUF60) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Poly U Binding Splicing Factor 60kDa (PUF60) CLIA Kit Customized Service Offer
n/a ELISA Kit for Poly U Binding Splicing Factor 60kDa (PUF60) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Poly U Binding Splicing Factor 60kDa (PUF60) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Poly U Binding Splicing Factor 60kDa (PUF60) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Poly U Binding Splicing Factor 60kDa (PUF60) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Poly U Binding Splicing Factor 60kDa (PUF60) CLIA Kit Customized Service Offer
n/a ELISA Kit for Poly U Binding Splicing Factor 60kDa (PUF60) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Poly U Binding Splicing Factor 60kDa (PUF60) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Poly U Binding Splicing Factor 60kDa (PUF60) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Poly U Binding Splicing Factor 60kDa (PUF60) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Poly U Binding Splicing Factor 60kDa (PUF60) CLIA Kit Customized Service Offer
n/a ELISA Kit for Poly U Binding Splicing Factor 60kDa (PUF60) ELISA Kit Customized Service Offer
  1. "The FBP interacting repressor targets TFIIH to inhibit activated transcription."Mol. Cell 5:331-341(2000) [PubMed] [Europe PMC] [Abstract]
  2. "Interaction cloning and characterization of RoBPI, a novel protein binding to human Ro ribonucleoproteins."RNA 6:66-78(2000) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "PUF60: a novel U2AF65-related splicing activity."RNA 5:1548-1560(1999) [PubMed] [Europe PMC] [Abstract]
  6. "Defective interplay of activators and repressors with TFIH in xeroderma pigmentosum."Cell 104:353-363(2001) [PubMed] [Europe PMC] [Abstract]
  7. "An essential role of alternative splicing of c-myc suppressor FUSE-binding protein-interacting repressor in carcinogenesis."Cancer Res. 66:1409-1417(2006) [PubMed] [Europe PMC] [Abstract]
  8. "The FUSE/FBP/FIR/TFIIH system is a molecular machine programming a pulse of c-myc expression."EMBO J. 25:2119-2130(2006) [PubMed] [Europe PMC] [Abstract]
  9. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  10. "Control of pre-mRNA splicing by the general splicing factors PUF60 and U2AF."PLoS ONE 2:E538-E538(2007) [PubMed] [Europe PMC] [Abstract]
  11. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  12. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  13. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  14. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  15. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  16. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  17. "Solution structure of the third RNA binding domain of FBP-interacting repressor, SIAHBP1."Submitted (APR-2007) to the PDB data bank
  18. "SCRIB and PUF60 are primary drivers of the multisystemic phenotypes of the 8q24.3 copy-number variant."Am. J. Hum. Genet. 93:798-811(2013) [PubMed] [Europe PMC] [Abstract]