≡

Thiopurine Methyltransferase (TPMT)

[Edit]

Thiopurine S Methyltransferase

Thiopurine Methyltransferase (TPMT)
CCC C821
HGNC 12014
MGI 98812
PubMed 20136357
RGD 1585162
UniProt P51580
Wiki TPMT
Thiopurine methyltransferase or thiopurine S-methyltransferase (TPMT) is an enzyme (EC 2.1.1.67) that methylates thiopurine compounds. The methyl donor is S-adenosyl-L-methionine, which is converted to S-adenosyl-L-homocysteine.
This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals. A pseudogene for this locus is located on chromosome 18q.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC821Hu01 Recombinant Thiopurine Methyltransferase (TPMT) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC821Hu01 Polyclonal Antibody to Thiopurine Methyltransferase (TPMT) WB; IHC; ICC; IP.
MAC821Hu22 Monoclonal Antibody to Thiopurine Methyltransferase (TPMT) WB; IHC; ICC; IP.
Assay Kits SEC821Hu ELISA Kit for Thiopurine Methyltransferase (TPMT) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Thiopurine Methyltransferase (TPMT) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Thiopurine Methyltransferase (TPMT) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Thiopurine Methyltransferase (TPMT) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Thiopurine Methyltransferase (TPMT) CLIA Kit Customized Service Offer
n/a ELISA Kit for Thiopurine Methyltransferase (TPMT) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Thiopurine Methyltransferase (TPMT) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Thiopurine Methyltransferase (TPMT) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Thiopurine Methyltransferase (TPMT) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Thiopurine Methyltransferase (TPMT) CLIA Kit Customized Service Offer
n/a ELISA Kit for Thiopurine Methyltransferase (TPMT) ELISA Kit Customized Service Offer
  1. "Human thiopurine methyltransferase: molecular cloning and expression of T84 colon carcinoma cell cDNA."Mol. Pharmacol. 43:878-887(1993) [PubMed] [Europe PMC] [Abstract]
  2. "Thiopurine methyltransferase pharmacogenetics. Cloning of human liver cDNA and a processed pseudogene on human chromosome 18q21.1."Drug Metab. Dispos. 23:398-405(1995) [PubMed] [Europe PMC] [Abstract]
  3. "Thiopurine methyltransferase pharmacogenetics: human gene cloning and characterization of a common polymorphism."DNA Cell Biol. 15:17-30(1996) [PubMed] [Europe PMC] [Abstract]
  4. "Promoter and intronic sequences of the human thiopurine S-methyltransferase (TPMT) gene isolated from a human PAC1 genomic library."Pharm. Res. 14:1672-1678(1997) [PubMed] [Europe PMC] [Abstract]
  5. "Human thiopurine methyltransferase pharmacogenetics: gene sequence polymorphisms."Clin. Pharmacol. Ther. 62:60-73(1997) [PubMed] [Europe PMC] [Abstract]
  6. "The DNA sequence and analysis of human chromosome 6." Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  8. "Detection of known and new mutations in the thiopurine S-methyltransferase gene by single-strand conformation polymorphism analysis."Hum. Mutat. 12:177-185(1998) [PubMed] [Europe PMC] [Abstract]
  9. "Structural basis of substrate recognition in thiopurine s-methyltransferase."Biochemistry 47:6216-6225(2008) [PubMed] [Europe PMC] [Abstract]
  10. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  11. "Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."Proteomics 8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract]
  12. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  13. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  14. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  15. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  16. "Structural basis of allele variation of human thiopurine-S-methyltransferase."Proteins 67:198-208(2007) [PubMed] [Europe PMC] [Abstract]
  17. "A single point mutation leading to loss of catalytic activity in human thiopurine S-methyltransferase."Proc. Natl. Acad. Sci. U.S.A. 92:949-953(1995) [PubMed] [Europe PMC] [Abstract]
  18. "Thiopurine S-methyltransferase deficiency: two nucleotide transitions define the most prevalent mutant allele associated with loss of catalytic activity in Caucasians."Am. J. Hum. Genet. 58:694-702(1996) [PubMed] [Europe PMC] [Abstract]
  19. "Azathioprine-induced severe pancytopenia due to a homozygous two-point mutation of the thiopurine methyltransferase gene in a patient with juvenile HLA-B27-associated spondylarthritis."Arthritis Rheum. 40:1896-1898(1997) [PubMed] [Europe PMC] [Abstract]
  20. "Enhanced proteolysis of thiopurine S-methyltransferase (TPMT) encoded by mutant alleles in humans (TPMT*3A, TPMT*2): mechanisms for the genetic polymorphism of TPMT activity."Proc. Natl. Acad. Sci. U.S.A. 94:6444-6449(1997) [PubMed] [Europe PMC] [Abstract]
  21. "Thiopurine methyltransferase alleles in British and Ghanaian populations."Hum. Mol. Genet. 8:367-370(1999) [PubMed] [Europe PMC] [Abstract]
  22. "Polymorphism of the thiopurine S-methyltransferase gene in African-Americans."Hum. Mol. Genet. 8:371-376(1999) [PubMed] [Europe PMC] [Abstract]
  23. "The frequency and distribution of thiopurine methyltransferase alleles in Caucasian and Asian populations."Pharmacogenetics 9:37-42(1999) [PubMed] [Europe PMC] [Abstract]
  24. "Genetic analysis of thiopurine methyltransferase polymorphism in a Japanese population."Mutat. Res. 448:91-95(2000) [PubMed] [Europe PMC] [Abstract]
  25. "Thiopurine S-methyltransferase pharmacogenetics: variant allele functional and comparative genomics."Pharmacogenet. Genomics 15:801-815(2005) [PubMed] [Europe PMC] [Abstract]
  26. "Severe azathioprine-induced myelotoxicity in a kidney transplant patient with thiopurine S-methyltransferase-deficient genotype (TPMT*3A/*3C)."Transpl. Int. 18:623-625(2005) [PubMed] [Europe PMC] [Abstract]
  27. "Molecular analysis of thiopurine S-methyltransferase alleles in Taiwan aborigines and Taiwanese."J. Clin. Pharm. Ther. 31:93-98(2006) [PubMed] [Europe PMC] [Abstract]
  28. "Frequency of the thiopurine S-methyltransferase alleles in the ancient genetic population isolate of Sardinia."J. Clin. Pharm. Ther. 31:283-287(2006) [PubMed] [Europe PMC] [Abstract]