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Deoxyhypusine Synthase (DHPS)

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MIG13; DHS; DS; Migration-Inducing Gene 13

Deoxyhypusine Synthase (DHPS)
CCC C900
HGNC 2869
MGI 2683592
PubMed 16023986
RGD 1303326
UniProt P49366
Wiki DHPS
The unusual amino acid hypusine is formed posttranslationally and is only found in a single cellular protein, eukaryotic translation initiation factor 5A.
In the first step of hypusine biosynthesis, deoxyhypusine synthase catalyzes the NAD-dependent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the EIF5A precursor protein to form the intermediate deoxyhypusine residue. This gene consists of nine exons spanning 6.6 kb. Three transcript variants have been isolated. However, only transcript variant 1 encodes an active protein. The shorter variants may act as modulating factors of DHPS activity.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC900Hu01 Recombinant Deoxyhypusine Synthase (DHPS) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC900Hu01 Polyclonal Antibody to Deoxyhypusine Synthase (DHPS) WB; IHC; ICC; IP.
Assay Kits SEC900Hu ELISA Kit for Deoxyhypusine Synthase (DHPS) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC900Mu01 Recombinant Deoxyhypusine Synthase (DHPS) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC900Mu01 Polyclonal Antibody to Deoxyhypusine Synthase (DHPS) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Deoxyhypusine Synthase (DHPS) CLIA Kit Customized Service Offer
n/a ELISA Kit for Deoxyhypusine Synthase (DHPS) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Deoxyhypusine Synthase (DHPS) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Deoxyhypusine Synthase (DHPS) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Deoxyhypusine Synthase (DHPS) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Deoxyhypusine Synthase (DHPS) CLIA Kit Customized Service Offer
n/a ELISA Kit for Deoxyhypusine Synthase (DHPS) ELISA Kit Customized Service Offer
  1. "Cloning and expression of human deoxyhypusine synthase cDNA. Structure-function studies with the recombinant enzyme and mutant proteins."J. Biol. Chem. 270:22386-22392(1995) [PubMed] [Europe PMC] [Abstract]
  2. "Molecular characterization of a cDNA encoding functional human deoxyhypusine synthase and chromosomal mapping of the corresponding gene locus."FEBS Lett. 378:195-198(1996) [PubMed] [Europe PMC] [Abstract]
  3. "Molecular cloning and functional expression of human deoxyhypusine synthase cDNA based on expressed sequence tag information."Biochem. J. 315:429-434(1996) [PubMed] [Europe PMC] [Abstract]
  4. "Localization and genomic structure of human deoxyhypusine synthase gene on chromosome 19p13.2-distal 19p13.1."Gene 215:153-157(1998) [PubMed] [Europe PMC] [Abstract]
  5. "Large-scale concatenation cDNA sequencing."Genome Res. 7:353-358(1997) [PubMed] [Europe PMC] [Abstract]
  6. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  7. "The DNA sequence and biology of human chromosome 19." Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  9. "Purification and characterization of human deoxyhypusine synthase from HeLa cells."FEBS Lett. 364:207-210(1995) [PubMed] [Europe PMC] [Abstract]
  10. "Enzyme-substrate intermediate at a specific lysine residue is required for deoxyhypusine synthesis. The role of Lys329 in human deoxyhypusine synthase."J. Biol. Chem. 272:32679-32685(1997) [PubMed] [Europe PMC] [Abstract]
  11. "Structure-function studies of human deoxyhypusine synthase: identification of amino acid residues critical for the binding of spermidine and NAD."Biochem. J. 355:841-849(2001) [PubMed] [Europe PMC] [Abstract]
  12. "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
  13. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  14. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  15. "Crystal structure of the NAD complex of human deoxyhypusine synthase: an enzyme with a ball-and-chain mechanism for blocking the active site."Structure 6:23-32(1998) [PubMed] [Europe PMC] [Abstract]
  16. "A new crystal structure of deoxyhypusine synthase reveals the configuration of the active enzyme and of an enzyme.NAD.inhibitor ternary complex."J. Biol. Chem. 279:28697-28705(2004) [PubMed] [Europe PMC] [Abstract]