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Ribonuclease A2 (RNASE2)

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Rnase-A2; Rnase2; EDN; Ribonuclease US; Ribonuclease,RNase A Family,2; Liver,Eosinophil-Derived Neurotoxin; MNon-secretory ribonuclease

Ribonuclease A2 (RNASE2)
CCC D169
HGNC 10045
MGI 1858238
PubMed 18673278
RGD 1359432
UniProt P10153
Wiki RNASE2
Eosinophil-derived neurotoxin is a distinct cationic protein of the eosinophil's large specific granule known primarily for its ability to induce ataxia, paralysis, and central nervous system cellular degeneration in experimental animals (Gordon phenomenon). Rosenberg et al. (1989) isolated a 725-bp cDNA clone for EDN. The open reading frame encodes a 134-amino acid polypeptide with a molecular mass of 15.5 kD and a 27-residue N-terminal hydrophobic leader sequence. The sequence of the mature polypeptide was identical to that reported for human urinary ribonuclease and to the N-terminal sequence of human liver ribonuclease. Similarities to the ribonucleases of pancreas and angiogenin indicate that EDN belongs to the ribonuclease multigene family. This gene is also symbolized RNS2 for ribonuclease 2.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPD169Hu01 Recombinant Ribonuclease A2 (RNASE2) Positive Control; Immunogen; SDS-PAGE; WB.
CPD169Hu21 OVA Conjugated Ribonuclease A2 (RNASE2) Immunogen; SDS-PAGE; WB.
Antibodies PAD169Hu01 Polyclonal Antibody to Ribonuclease A2 (RNASE2) WB; IHC
MAD169Hu21 Monoclonal Antibody to Ribonuclease A2 (RNASE2) WB; IHC
MAD169Hu22 Monoclonal Antibody to Ribonuclease A2 (RNASE2) WB; IHC; ICC; IP.
MAD169Hu23 Monoclonal Antibody to Ribonuclease A2 (RNASE2) WB; IHC
MAD169Hu25 Monoclonal Antibody to Ribonuclease A2 (RNASE2) WB; IHC; ICC; IP.
MAD169Hu24 Monoclonal Antibody to Ribonuclease A2 (RNASE2) WB; IHC; ICC; IP.
MAD169Hu29 Monoclonal Antibody to Ribonuclease A2 (RNASE2) WB; IHC; ICC; IP.
MAD169Hu27 Monoclonal Antibody to Ribonuclease A2 (RNASE2) WB; IHC; ICC; IP.
Assay Kits SED169Hu ELISA Kit for Ribonuclease A2 (RNASE2) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPD169Mu01 Recombinant Ribonuclease A2 (RNASE2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAD169Mu01 Polyclonal Antibody to Ribonuclease A2 (RNASE2) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Ribonuclease A2 (RNASE2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Ribonuclease A2 (RNASE2) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPD169Ra01 Recombinant Ribonuclease A2 (RNASE2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAD169Ra01 Polyclonal Antibody to Ribonuclease A2 (RNASE2) WB,IHC
Assay Kits n/a CLIA Kit for Ribonuclease A2 (RNASE2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Ribonuclease A2 (RNASE2) ELISA Kit Customized Service Offer
  1. "Sequence of human eosinophil-derived neurotoxin cDNA: identity of deduced amino acid sequence with human nonsecretory ribonucleases."Gene 83:161-167(1989) [PubMed] [Europe PMC] [Abstract]
  2. "Eosinophil cationic protein cDNA. Comparison with other toxic cationic proteins and ribonucleases."J. Immunol. 143:952-955(1989) [PubMed] [Europe PMC] [Abstract]
  3. "Molecular cloning of the human eosinophil-derived neurotoxin: a member of the ribonuclease gene family."Proc. Natl. Acad. Sci. U.S.A. 86:4460-4464(1989) [PubMed] [Europe PMC] [Abstract]
  4. "Structure and chromosome localization of the human eosinophil-derived neurotoxin and eosinophil cationic protein genes: evidence for intronless coding sequences in the ribonuclease gene superfamily."Genomics 7:535-546(1990) [PubMed] [Europe PMC] [Abstract]
  5. "Sequence variation at two eosinophil-associated ribonuclease loci in humans."Genetics 156:1949-1958(2000) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Amino acid sequence of the nonsecretory ribonuclease of human urine."Biochemistry 27:4530-4538(1988) [PubMed] [Europe PMC] [Abstract]
  8. "Biochemical and functional similarities between human eosinophil-derived neurotoxin and eosinophil cationic protein: homology with ribonuclease."Proc. Natl. Acad. Sci. U.S.A. 83:3146-3150(1986) [PubMed] [Europe PMC] [Abstract]
  9. "Purification and properties of bovine kidney ribonucleases."J. Biochem. 97:923-934(1985) [PubMed] [Europe PMC] [Abstract]
  10. "Purification and characterization of a ribonuclease from human liver."J. Biol. Chem. 263:16125-16131(1988) [PubMed] [Europe PMC] [Abstract]
  11. "Characterization of a unique nonsecretory ribonuclease from urine of pregnant women."J. Biochem. 111:325-330(1992) [PubMed] [Europe PMC] [Abstract]
  12. "Characterisation of UGP and its relationship with beta-core fragment."Br. J. Cancer 67:686-692(1993) [PubMed] [Europe PMC] [Abstract]
  13. "New type of linkage between a carbohydrate and a protein: C-glycosylation of a specific tryptophan residue in human RNase Us."Biochemistry 33:13524-13530(1994) [PubMed] [Europe PMC] [Abstract]
  14. "The hexopyranosyl residue that is C-glycosidically linked to the side chain of tryptophan-7 in human RNase Us is alpha-mannopyranose."Biochemistry 34:11785-11789(1995) [PubMed] [Europe PMC] [Abstract]
  15. "Recognition signal for C-mannosylation of Trp-7 in RNase 2 consists of sequence Trp-x-x-Trp."Mol. Biol. Cell 9:301-309(1998) [PubMed] [Europe PMC] [Abstract]
  16. "Mutational analysis of the complex of human RNase inhibitor and human eosinophil-derived neurotoxin (RNase 2)."Biochemistry 42:1451-1459(2003) [PubMed] [Europe PMC] [Abstract]
  17. "Eosinophil-derived neurotoxin (EDN), an antimicrobial protein with chemotactic activities for dendritic cells."Blood 102:3396-3403(2003) [PubMed] [Europe PMC] [Abstract]
  18. "Post-translational tyrosine nitration of eosinophil granule toxins mediated by eosinophil peroxidase." J. Biol. Chem. 283:28629-28640(2008) [PubMed] [Europe PMC] [Abstract]
  19. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  20. "X-ray crystallographic structure of recombinant eosinophil-derived neurotoxin at 1.83-A resolution."J. Mol. Biol. 260:540-552(1996) [PubMed] [Europe PMC] [Abstract]
  21. "Mapping the ribonucleolytic active site of eosinophil-derived neurotoxin (EDN). High resolution crystal structures of EDN complexes with adenylic nucleotide inhibitors."J. Biol. Chem. 276:15009-15017(2001) [PubMed] [Europe PMC] [Abstract]
  22. "Molecular recognition of human eosinophil-derived neurotoxin (RNase 2) by placental ribonuclease inhibitor."J. Mol. Biol. 347:637-655(2005) [PubMed] [Europe PMC] [Abstract]
  23. "Crystal structures of eosinophil-derived neurotoxin (EDN) in complex with the inhibitors 5'-ATP, Ap3A, Ap4A, and Ap5A."Biochemistry 45:416-426(2006) [PubMed] [Europe PMC] [Abstract]