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Chromodomain Helicase DNA Binding Protein 4 (CHD4)

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Mi-2b, Mi2-BETA; ATP-dependent helicase CHD4; Mi-2 autoantigen 218 kDa protein

Chromodomain Helicase DNA Binding Protein 4 (CHD4)
CCC D202
HGNC 1919
MGI 1344380
PubMed 23697937
RGD 620064
UniProt Q14839
Wiki CHD4
Chromodomain-helicase-DNA-binding protein 4 is an enzymebelongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein.Seelig et al. (1996) noted that the Mi2-alpha (CHD3) and Mi2-beta proteins react with most or all dermatomyositis patient anti-Mi2 sera. While these proteins are distinct, they have stretches of identical sequences that could result in shared epitopes. The authors stated that Mi2-alpha and Mi2-beta contain a PHD motif, which is a zinc finger-like motif with a cys4-his-cys3 pattern and which is thought to be a DNA- or RNA-binding domain.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPD202Hu01 Recombinant Chromodomain Helicase DNA Binding Protein 4 (CHD4) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies n/a Monoclonal Antibody to Chromodomain Helicase DNA Binding Protein 4 (CHD4) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Chromodomain Helicase DNA Binding Protein 4 (CHD4) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Chromodomain Helicase DNA Binding Protein 4 (CHD4) CLIA Kit Customized Service Offer
n/a ELISA Kit for Chromodomain Helicase DNA Binding Protein 4 (CHD4) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPD202Mu01 Recombinant Chromodomain Helicase DNA Binding Protein 4 (CHD4) Positive Control; Immunogen; SDS-PAGE; WB.
RPD202Mu02 Recombinant Chromodomain Helicase DNA Binding Protein 4 (CHD4) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAD202Mu01 Polyclonal Antibody to Chromodomain Helicase DNA Binding Protein 4 (CHD4) WB; IHC; ICC; IP.
PAD202Mu02 Polyclonal Antibody to Chromodomain Helicase DNA Binding Protein 4 (CHD4) WB; IHC; ICC; IP.
Assay Kits SED202Mu ELISA Kit for Chromodomain Helicase DNA Binding Protein 4 (CHD4) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Chromodomain Helicase DNA Binding Protein 4 (CHD4) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Chromodomain Helicase DNA Binding Protein 4 (CHD4) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Chromodomain Helicase DNA Binding Protein 4 (CHD4) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Chromodomain Helicase DNA Binding Protein 4 (CHD4) CLIA Kit Customized Service Offer
n/a ELISA Kit for Chromodomain Helicase DNA Binding Protein 4 (CHD4) ELISA Kit Customized Service Offer
  1. "The major dermatomyositis specific Mi-2 autoantigen is a presumed helicase involved in transcriptional activation."Arthritis Rheum. 38:1389-1399(1995) [PubMed] [Europe PMC] [Abstract]
  2. "The finished DNA sequence of human chromosome 12." Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Chromatin deacetylation by an ATP-dependent nucleosome remodelling complex."Nature 395:917-921(1998) [PubMed] [Europe PMC] [Abstract]
  5. "Molecular association between ATR and two components of the nucleosome remodeling and deacetylating complex, HDAC2 and CHD4."Biochemistry 38:14711-14717(1999) [PubMed] [Europe PMC] [Abstract]
  6. "Ikaros DNA-binding proteins direct formation of chromatin remodeling complexes in lymphocytes."Immunity 10:345-355(1999) [PubMed] [Europe PMC] [Abstract]
  7. "Mi-2 beta associates with BRG1 and RET finger protein at the distinct regions with transcriptional activating and repressing abilities."J. Biol. Chem. 278:51638-51645(2003) [PubMed] [Europe PMC] [Abstract]
  8. "MTA3 and the Mi-2/NuRD complex regulate cell fate during B lymphocyte differentiation."Cell 119:75-86(2004) [PubMed] [Europe PMC] [Abstract]
  9. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  10. "Chromatin remodeling proteins interact with pericentrin to regulate centrosome integrity."Mol. Biol. Cell 18:3667-3680(2007) [PubMed] [Europe PMC] [Abstract]
  11. "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
  12. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  13. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  14. "ZIP: a novel transcription repressor, represses EGFR oncogene and suppresses breast carcinogenesis."EMBO J. 28:2763-2776(2009) [PubMed] [Europe PMC] [Abstract]
  15. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  16. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  17. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  18. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  19. "The Brd4 extraterminal domain confers transcription activation independent of pTEFb by recruiting multiple proteins, including NSD3."Mol. Cell. Biol. 31:2641-2652(2011) [PubMed] [Europe PMC] [Abstract]
  20. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  21. "Senataxin, defective in the neurodegenerative disorder ataxia with oculomotor apraxia 2, lies at the interface of transcription and the DNA damage response."Mol. Cell. Biol. 33:406-417(2013) [PubMed] [Europe PMC] [Abstract]
  22. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  23. "Engineering a protein scaffold from a PHD finger."Structure 11:803-813(2003) [PubMed] [Europe PMC] [Abstract]
  24. "Solution structures of the chromo domain of human chromodomain helicase-DNA-binding protein 4."Submitted (AUG-2007) to the PDB data bank