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Leukotriene A4 Hydrolase (LTA4H)

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LTA4 Hydrolase

Leukotriene A4 Hydrolase (LTA4H)
CCC D236
HGNC 6710
MGI 96836
PubMed 24573245
RGD 1311333
UniProt P09960
Wiki LTA4H
Minami et al. (1987) reported the full-length cDNA and complete primary structure of human LTA4 hydrolase. This was the first report of the molecular cloning of an enzyme involved in the biosynthesis of eicosanoids. Funk et al. (1987) isolated a cDNA clone corresponding to leukotriene A4 hydrolase from a human lung lambda-gt11 expression library by immunoscreening with a polyclonal antiserum. Several additional clones from human lung and placenta cDNA lambda-gt11 libraries were obtained by plaque hybridization with the (32)P-labeled lung cDNA clone. One of the clones had an insert of 1,910 basepairs that contained a complete protein-coding region. From the deduced primary structure, leukotriene A4 hydrolase is a 610-amino acid protein with a calculated molecular weight of 69,140.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPD236Hu01 Recombinant Leukotriene A4 Hydrolase (LTA4H) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAD236Hu01 Polyclonal Antibody to Leukotriene A4 Hydrolase (LTA4H) WB; IHC; ICC; IP.
MAD236Hu21 Monoclonal Antibody to Leukotriene A4 Hydrolase (LTA4H) WB; IHC; ICC; IP.
Assay Kits SED236Hu ELISA Kit for Leukotriene A4 Hydrolase (LTA4H) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPD236Mu01 Recombinant Leukotriene A4 Hydrolase (LTA4H) Positive Control; Immunogen; SDS-PAGE; WB.
RPD236Mu02 Recombinant Leukotriene A4 Hydrolase (LTA4H) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAD236Mu01 Polyclonal Antibody to Leukotriene A4 Hydrolase (LTA4H) WB; IHC; ICC; IP.
PAD236Mu02 Polyclonal Antibody to Leukotriene A4 Hydrolase (LTA4H) WB; IHC; ICC; IP.
Assay Kits SED236Mu ELISA Kit for Leukotriene A4 Hydrolase (LTA4H) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Leukotriene A4 Hydrolase (LTA4H) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Leukotriene A4 Hydrolase (LTA4H) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Leukotriene A4 Hydrolase (LTA4H) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Leukotriene A4 Hydrolase (LTA4H) CLIA Kit Customized Service Offer
n/a ELISA Kit for Leukotriene A4 Hydrolase (LTA4H) ELISA Kit Customized Service Offer
  1. "Molecular cloning of a cDNA coding for human leukotriene A4 hydrolase. Complete primary structure of an enzyme involved in eicosanoid synthesis."J. Biol. Chem. 262:13873-13876(1987) [PubMed] [Europe PMC] [Abstract]
  2. "Molecular cloning and amino acid sequence of leukotriene A4 hydrolase."Proc. Natl. Acad. Sci. U.S.A. 84:6677-6681(1987) [PubMed] [Europe PMC] [Abstract]
  3. "Cloning and characterization of the human leukotriene A4 hydrolase gene."Eur. J. Biochem. 231:65-71(1995) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The full-ORF clone resource of the German cDNA consortium."BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
  6. "The finished DNA sequence of human chromosome 12." Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  8. "Leukotriene A4 hydrolase in the human B-lymphocytic cell line Raji: indications of catalytically divergent forms of the enzyme."Arch. Biochem. Biophys. 287:167-174(1991) [PubMed] [Europe PMC] [Abstract]
  9. "Leukotriene A4 hydrolase in human leukocytes. Purification and properties."J. Biol. Chem. 259:12339-12345(1984) [PubMed] [Europe PMC] [Abstract]
  10. "Leukotriene A4 hydrolase: mapping of a henicosapeptide involved in mechanism-based inactivation."Proc. Natl. Acad. Sci. U.S.A. 92:8383-8387(1995) [PubMed] [Europe PMC] [Abstract]
  11. "The human leukotriene A4 hydrolase gene is expressed in two alternatively spliced mRNA forms."Biochem. J. 314:733-737(1996) [PubMed] [Europe PMC] [Abstract]
  12. "Molecular evolution and zinc ion binding motif of leukotriene A4 hydrolase."Biochem. Biophys. Res. Commun. 171:216-221(1990) [PubMed] [Europe PMC] [Abstract]
  13. "Leukotriene A4 hydrolase: a zinc metalloenzyme."Biochem. Biophys. Res. Commun. 172:965-970(1990) [PubMed] [Europe PMC] [Abstract]
  14. "Leukotriene A4 hydrolase: determination of the three zinc-binding ligands by site-directed mutagenesis and zinc analysis."Proc. Natl. Acad. Sci. U.S.A. 88:7620-7624(1991) [PubMed] [Europe PMC] [Abstract]
  15. "Leukotriene A4 hydrolase, a bifunctional enzyme. Distinction of leukotriene A4 hydrolase and aminopeptidase activities by site-directed mutagenesis at Glu-297."FEBS Lett. 309:353-357(1992) [PubMed] [Europe PMC] [Abstract]
  16. "Leukotriene A4 hydrolase: abrogation of the peptidase activity by mutation of glutamic acid-296."Proc. Natl. Acad. Sci. U.S.A. 89:9141-9145(1992) [PubMed] [Europe PMC] [Abstract]
  17. "Regulation of leukotriene A4 hydrolase activity in endothelial cells by phosphorylation."J. Biol. Chem. 272:31865-31871(1997) [PubMed] [Europe PMC] [Abstract]
  18. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  19. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  20. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  21. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  22. "Crystal structure of human leukotriene A(4) hydrolase, a bifunctional enzyme in inflammation."Nat. Struct. Biol. 8:131-135(2001) [PubMed] [Europe PMC] [Abstract]
  23. "Crystal structures of leukotriene A4 hydrolase in complex with captopril and two competitive tight-binding inhibitors."FASEB J. 16:1648-1650(2002) [PubMed] [Europe PMC] [Abstract]
  24. "Leukotriene A4 hydrolase/aminopeptidase. Glutamate 271 is a catalytic residue with specific roles in two distinct enzyme mechanisms."J. Biol. Chem. 277:1398-1404(2002) [PubMed] [Europe PMC] [Abstract]
  25. "Leukotriene A4 hydrolase: selective abrogation of leukotriene B4 formation by mutation of aspartic acid 375."Proc. Natl. Acad. Sci. U.S.A. 99:4215-4220(2002) [PubMed] [Europe PMC] [Abstract]
  26. "Leukotriene A4 hydrolase: identification of a common carboxylate recognition site for the epoxide hydrolase and aminopeptidase substrates."J. Biol. Chem. 279:27376-27382(2004) [PubMed] [Europe PMC] [Abstract]
  27. "Structure-based dissection of the active site chemistry of leukotriene A4 hydrolase: implications for M1 aminopeptidases and inhibitor design."Chem. Biol. 15:920-929(2008) [PubMed] [Europe PMC] [Abstract]
  28. "Discovery of leukotriene A4 hydrolase inhibitors using metabolomics biased fragment crystallography."J. Med. Chem. 52:4694-4715(2009) [PubMed] [Europe PMC] [Abstract]